Hi Kahkashan, This paper shows a nice example with specific crosslinks in the nucleosome: https://doi.org/10.1016/j.jmb.2017.10.029 <https://doi.org/10.1016/j.jmb.2017.10.029> Of course, having a high-resolution structure helps a lot to design specific crosslinks like they did… You may not have one if you work on determining a novel structure. But I hope this paper and references within will be helpful to you.
Cheers, Guillaume > On 16 Mar 2020, at 08:58, Firdous Tarique <[email protected]> wrote: > > Hi > > I have a weird question. Is it possible to incorporate or mutate amino acid > residues at the interface of two interacting surfaces in a multi subunit > protein complexes hoping it to form a disulfide bond making the over all > complex more stable for downstream structural studies ? > > Is there any sort of literature available where creating these types of > modification or kind of protein engineering helped in making a more stable > complex, eventually helping in further structural studies by x-ray or cryoem ? > > Best > > kahkashan > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
