Dear All, We are trying to solve a structure of a protein, for which we have collected five different home source data at 3.2-3.5 Ang resolution. We are processing the data using iMOSFLM and the program suggests P3 (and related) space groups for all the data. We are able to get a solution with one data (twinned) in C2 space group with Rw/Rf of 26/28%. However, the same solution can not be obtained using other four data. Interestingly, one of these four data is not twinned. The only common thing in these four data, I find, is that they are crystallized in the same crystallization condition, whereas, the data which gives solution is crystallized in another condition.
The template has 69% sequence identity with the target protein. Any suggestion to troubleshoot this would be appreciated. With best regards, Suraj -- *Suraj Kr. Mandal* Research Scholar Structural and Computational Biology Lab Department of Biosciences and Bioengineering Indian Institute of Technology (IIT), Guwahati Guwahati, Assam 781039 Ph.: 09678244566 E.mail: [email protected] [email protected] ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
