Hello, have you tried the 'pointless' option to use one of the structures/datasets as a reference, then there should not be a need to do MR on the others, since they will all be indexed consistently. Sorry if that is what you meant below. Cheers, Jon Cooper.
Sent from ProtonMail mobile -------- Original Message -------- On 11 Dec 2020, 12:01, Suraj Kumar mandal wrote: > Dear Sir, > > Yes, we have checked handedness also. We are using the same MR solution with > other data. > > With best regards > Suraj > > On Fri, Dec 11, 2020 at 4:56 PM srajan kapoor <[email protected]> wrote: > >> Have you checked for handedness to solve this problem?. >> You can also try to use the structure that you have solved for molecular >> Replacement of the other crystals. >> I have only two things in mind currently. I hope it will work for you. >> >> On Fri, Dec 11, 2020, 4:48 PM Suraj Kumar mandal <[email protected]> >> wrote: >> >>> Dear All, >>> >>> We are trying to solve a structure of a protein, for which we have >>> collected five different home source data at 3.2-3.5 Ang resolution. We are >>> processing the data using iMOSFLM and the program suggests P3 (and related) >>> space groups for all the data. We are able to get a solution with one data >>> (twinned) in C2 space group with Rw/Rf of 26/28%. However, the same >>> solution can not be obtained using other four data. Interestingly, one of >>> these four data is not twinned. The only common thing in these four data, I >>> find, is that they are crystallized in the same crystallization condition, >>> whereas, the data which gives solution is crystallized in another condition. >>> >>> The template has 69% sequence identity with the target protein. >>> >>> Any suggestion to troubleshoot this would be appreciated. >>> >>> With best regards, >>> Suraj >>> >>> -- >>> >>> Suraj Kr. Mandal >>> Research Scholar >>> Structural and Computational Biology Lab >>> Department of Biosciences and Bioengineering >>> Indian Institute of Technology (IIT), Guwahati >>> Guwahati, Assam 781039 >>> Ph.: 09678244566 >>> E.mail: [email protected] [email protected] >>> >>> --------------------------------------------------------------- >>> >>> To unsubscribe from the CCP4BB list, click the following link: >>> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > -- > > Suraj Kr. Mandal > Research Scholar > Structural and Computational Biology Lab > Department of Biosciences and Bioengineering > Indian Institute of Technology (IIT), Guwahati > Guwahati, Assam 781039 > Ph.: 09678244566 > E.mail: [email protected] [email protected] > > --------------------------------------------------------------- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
