Dear Sir, Yes, we have checked handedness also. We are using the same MR solution with other data.
With best regards Suraj On Fri, Dec 11, 2020 at 4:56 PM srajan kapoor <[email protected]> wrote: > Have you checked for handedness to solve this problem?. > You can also try to use the structure that you have solved for molecular > Replacement of the other crystals. > I have only two things in mind currently. I hope it will work for you. > > On Fri, Dec 11, 2020, 4:48 PM Suraj Kumar mandal <[email protected]> > wrote: > >> Dear All, >> >> We are trying to solve a structure of a protein, for which we have >> collected five different home source data at 3.2-3.5 Ang resolution. We are >> processing the data using iMOSFLM and the program suggests P3 (and related) >> space groups for all the data. We are able to get a solution with one data >> (twinned) in C2 space group with Rw/Rf of 26/28%. However, the same >> solution can not be obtained using other four data. Interestingly, one of >> these four data is not twinned. The only common thing in these four data, I >> find, is that they are crystallized in the same crystallization condition, >> whereas, the data which gives solution is crystallized in another condition. >> >> The template has 69% sequence identity with the target protein. >> >> Any suggestion to troubleshoot this would be appreciated. >> >> With best regards, >> Suraj >> >> -- >> *Suraj Kr. Mandal* >> Research Scholar >> Structural and Computational Biology Lab >> Department of Biosciences and Bioengineering >> Indian Institute of Technology (IIT), Guwahati >> Guwahati, Assam 781039 >> Ph.: 09678244566 >> E.mail: [email protected] >> [email protected] >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >> > -- *Suraj Kr. Mandal* Research Scholar Structural and Computational Biology Lab Department of Biosciences and Bioengineering Indian Institute of Technology (IIT), Guwahati Guwahati, Assam 781039 Ph.: 09678244566 E.mail: [email protected] [email protected] ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
