Well - C2 is a sub cell for P3 so that isnt surprising, but a cell difference of 202 to 212 means it isnt isomorphous.. But an Rw Rf/ of 26/31 isnt bad for such low resolution data? Eleanor
On Fri, 11 Dec 2020 at 12:01, Suraj Kumar mandal <[email protected]> wrote: > Dear Sir, > > Yes, we have checked handedness also. We are using the same MR solution > with other data. > > > With best regards > Suraj > > > > On Fri, Dec 11, 2020 at 4:56 PM srajan kapoor <[email protected]> > wrote: > >> Have you checked for handedness to solve this problem?. >> You can also try to use the structure that you have solved for molecular >> Replacement of the other crystals. >> I have only two things in mind currently. I hope it will work for you. >> >> On Fri, Dec 11, 2020, 4:48 PM Suraj Kumar mandal <[email protected]> >> wrote: >> >>> Dear All, >>> >>> We are trying to solve a structure of a protein, for which we have >>> collected five different home source data at 3.2-3.5 Ang resolution. We are >>> processing the data using iMOSFLM and the program suggests P3 (and related) >>> space groups for all the data. We are able to get a solution with one data >>> (twinned) in C2 space group with Rw/Rf of 26/28%. However, the same >>> solution can not be obtained using other four data. Interestingly, one of >>> these four data is not twinned. The only common thing in these four data, I >>> find, is that they are crystallized in the same crystallization condition, >>> whereas, the data which gives solution is crystallized in another condition. >>> >>> The template has 69% sequence identity with the target protein. >>> >>> Any suggestion to troubleshoot this would be appreciated. >>> >>> With best regards, >>> Suraj >>> >>> -- >>> *Suraj Kr. Mandal* >>> Research Scholar >>> Structural and Computational Biology Lab >>> Department of Biosciences and Bioengineering >>> Indian Institute of Technology (IIT), Guwahati >>> Guwahati, Assam 781039 >>> Ph.: 09678244566 >>> E.mail: [email protected] >>> [email protected] >>> >>> ------------------------------ >>> >>> To unsubscribe from the CCP4BB list, click the following link: >>> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >>> >> > > -- > *Suraj Kr. Mandal* > Research Scholar > Structural and Computational Biology Lab > Department of Biosciences and Bioengineering > Indian Institute of Technology (IIT), Guwahati > Guwahati, Assam 781039 > Ph.: 09678244566 > E.mail: [email protected] > [email protected] > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
