Hi Shubhashish, How many molecules do you assume are in the asymmetric unit? You may have a very high solvent content and by trying to find multiple molecules, you spoil your solution. Also, did you do a thermal shift assay to make sure your protein is properly folded?
Best, Herman Von: CCP4 bulletin board <[email protected]> Im Auftrag von Shubhashish Chakraborty Gesendet: Donnerstag, 3. März 2022 05:44 An: [email protected] Betreff: [ccp4bb] MR solution not working Hello, I am trying to solve a dataset using molecular replacement. However, neither Phaser MR nor Molrep can give any solution. In Phaser, I have received an advisory that Top FTF has not packed. I have tried molecular replacement using the wild-type protein at different resolutions (I am working on a mutant). Also, I have truncated the loops from the input structure. However, none have worked. So, what can be the possible way to solve this data set? Thank you Shubhashish Chakraborty PhD JRF 2018 Structural and Molecular Biology Lab (Varma Lab) Advanced Centre for Treatment, Research and Education in Cancer (ACTREC) Khargar, Navi Mumbai E-mail: [email protected]<mailto:[email protected]> ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1<https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
