Hi Firdous, I have read a fair amount of papers about ATP-dependent chromatin remodelers in which ADP-BeF3 is often cited. It is made as follows (quoting from one of these papers): "ADP-BeF3 was prepared as a 10x stock (10 mM ADP, 30 mM BeSO4, 150 mM NaF, 10 mM MgCl2), freshly before use."
So the trick is to get all you need from a mixture of soluble compounds, instead of from only one poorly soluble salt. Not sure this will work the same with Al instead of Be, but it’s easy enough to try: use any known-soluble salt of Al instead of this BeSO4, and see if it works. NaF is extremely toxic, so use adequate protective measures (PPE, hood, etc.). Good luck, Guillaume > On 21 Jun 2022, at 19:38, Firdous Tarique <[email protected]> wrote: > > Hi > > Sorry for the off topic question but can anybody please suggest how to > dissolve Aluminium Fluoride in water ? I know it has little solubility in > water but have seen people using AF3 for their structural studies (ADP + AF3 > as ATP analogue). Is there any other solvent where they have made AF3 > solution or is there some trick to dissolve it in water?. > > Thanks > > Firdous > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > <https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
