Thanks everyone for their wonderful suggestions. Best
F On Wed, Jun 22, 2022 at 11:42 AM Patrick Loll <[email protected]> wrote: > Kevin and Guillaume said it all quite well. I just want to stress one > thing, namely that these solutions are metastable, and will eventually > precipitate (hours to days). > > In the case of the beryllium salts, the precipitation is due to slow > formation of metal hydroxide species. I have no direct experience with the > aluminum variants, but since aluminum is notorious for forming goopy > hydroxides, I would guess that the same issue pertains. So make the > solution right before using, try not to use a pH too far above neutrality, > and be very cautious about (for example) putting any of these solutions > into an FPLC. > > Pat > > > On 22 Jun 2022, at 12:15 PM, Dr. Kevin M Jude <[email protected]> > wrote: > > > > Sorry, I should have said ADPBeF3 (not ADPBeF4), and increase the NaF > proportion for ADPAlF4. Which in practice means reducing the concentrations > of everything else due to limited solubility of NaF. > > > > From: CCP4 bulletin board <[email protected]> on behalf of Dr. > Kevin M Jude <[email protected]> > > Date: Wednesday, June 22, 2022 at 9:10 AM > > To: [email protected] <[email protected]> > > Subject: Re: [ccp4bb] Solubility of Aluminum Fluoride > > > > I posted the following to ccp4bb in 2017 in response to a similar > question about ADPBeF4. For AlF4 you’ll increase the NaF stoichiometrically: > > > > reagent [stock] (mM) final concentration (mM) volume (µl) > > > BeCl2 1000 90 9 > > NaF 750 450 60 > > ADP, pH 8 100 30 30 > > H2O 1 > > total volume 100 > > > > To prepare ADP:Be:F (1:3:15) > > Prepare BeCl2 stock in the fume hood due to release of Cl2 gas > > > thoroughly mix BeCl2 and NaF > > Add ADP > > dilute with H2O to final volume > > > > This is done at room temperature and the reactions are fast. Careful > mixing (and adjusting the pH of the ADP beforehand) are important because > the BeCl2 stock will precipitate at neutral or elevated pH, and the BeFx > solution is quite acidic. > > > > > > > > --------------------------------------------------------------------------------------- > Patrick J. Loll, Ph. D. (he, him, his) > Professor of Biochemistry & Molecular Biology > Drexel University College of Medicine > Room 10-102 New College Building > 245 N. 15th St., Mailstop 497 > Philadelphia, PA 19102 USA > > (215) 762-7706 > [email protected] > [email protected] > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a > mailing list hosted by www.jiscmail.ac.uk, terms & conditions are > available at https://www.jiscmail.ac.uk/policyandsecurity/ > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
