I have never tried varying the order of mixing, but it seems the correct ADP-BeF3 complex was formed when mixing all three solutions just before use.
Guillaume > On 22 Jun 2022, at 02:12, Firdous Tarique <kahkashantari...@gmail.com> wrote: > > Thanks for your input. To avoid precipitation what about doing something like > this? For example, as we know: AlCl3 + 3NaF = AlF3 + 3NaCl > > Therefore, adding 100mM AlCl3 plus 300mM NaF to get 100mM of AlF3 solution, > then directly using this 100 mM stock of AlF3 for the final dilution, say 1 > or 2 mM in the reaction mix ? Or it is recommended to use AlCl3 and NaF > hoping to form AlF3 in the reaction mix. > > Best > > Faisal > > > On Tue, Jun 21, 2022 at 2:08 PM Guillaume Gaullier > <guillaume.gaull...@univ-tlse3.fr <mailto:guillaume.gaull...@univ-tlse3.fr>> > wrote: > Hi Firdous, > > I have read a fair amount of papers about ATP-dependent chromatin remodelers > in which ADP-BeF3 is often cited. It is made as follows (quoting from one of > these papers): "ADP-BeF3 was prepared as a 10x stock (10 mM ADP, 30 mM BeSO4, > 150 mM NaF, 10 mM MgCl2), freshly before use." > > So the trick is to get all you need from a mixture of soluble compounds, > instead of from only one poorly soluble salt. Not sure this will work the > same with Al instead of Be, but it’s easy enough to try: use any > known-soluble salt of Al instead of this BeSO4, and see if it works. NaF is > extremely toxic, so use adequate protective measures (PPE, hood, etc.). > > Good luck, > > Guillaume > > >> On 21 Jun 2022, at 19:38, Firdous Tarique <kahkashantari...@gmail.com >> <mailto:kahkashantari...@gmail.com>> wrote: >> >> Hi >> >> Sorry for the off topic question but can anybody please suggest how to >> dissolve Aluminium Fluoride in water ? I know it has little solubility in >> water but have seen people using AF3 for their structural studies (ADP + AF3 >> as ATP analogue). Is there any other solvent where they have made AF3 >> solution or is there some trick to dissolve it in water?. >> >> Thanks >> >> Firdous >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >> <https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
