Kevin and Guillaume said it all quite well. I just want to stress one thing, namely that these solutions are metastable, and will eventually precipitate (hours to days).
In the case of the beryllium salts, the precipitation is due to slow formation of metal hydroxide species. I have no direct experience with the aluminum variants, but since aluminum is notorious for forming goopy hydroxides, I would guess that the same issue pertains. So make the solution right before using, try not to use a pH too far above neutrality, and be very cautious about (for example) putting any of these solutions into an FPLC. Pat > On 22 Jun 2022, at 12:15 PM, Dr. Kevin M Jude <kj...@stanford.edu> wrote: > > Sorry, I should have said ADPBeF3 (not ADPBeF4), and increase the NaF > proportion for ADPAlF4. Which in practice means reducing the concentrations > of everything else due to limited solubility of NaF. > > From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Dr. Kevin M > Jude <kj...@stanford.edu> > Date: Wednesday, June 22, 2022 at 9:10 AM > To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> > Subject: Re: [ccp4bb] Solubility of Aluminum Fluoride > > I posted the following to ccp4bb in 2017 in response to a similar question > about ADPBeF4. For AlF4 you’ll increase the NaF stoichiometrically: > > reagent [stock] (mM) final concentration (mM) volume (µl) > BeCl2 1000 90 9 > NaF 750 450 60 > ADP, pH 8 100 30 30 > H2O 1 > total volume 100 > > To prepare ADP:Be:F (1:3:15) > Prepare BeCl2 stock in the fume hood due to release of Cl2 gas > > thoroughly mix BeCl2 and NaF > Add ADP > dilute with H2O to final volume > > This is done at room temperature and the reactions are fast. Careful mixing > (and adjusting the pH of the ADP beforehand) are important because the BeCl2 > stock will precipitate at neutral or elevated pH, and the BeFx solution is > quite acidic. > > > --------------------------------------------------------------------------------------- Patrick J. Loll, Ph. D. (he, him, his) Professor of Biochemistry & Molecular Biology Drexel University College of Medicine Room 10-102 New College Building 245 N. 15th St., Mailstop 497 Philadelphia, PA 19102 USA (215) 762-7706 pjl...@gmail.com pj...@drexel.edu ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
