> On 3 Jul 2026, at 16:43, Kikuti Carlos 
> <[email protected]> wrote:
> 
> And I am aware that most of the time, analysis and molecular dynamics 
> programs do not take B-factors in account.

At the risk of diverging from the original topic of discussion - but it’s too 
good to resist.

Many years ago I was tangentially involved with a computational biologist that 
was (as far as I can remember)  doing normal mode analysis based on some of our 
structures including one I’d built and refined. On several occasions he was a 
bit rude about the quality of the model building and when eventually pressed 
said “and they even built some atoms *on top of* other atoms”. It turned out 
the scripts that were used to prepare the input coordinates ignored the altLoc 
column and I had built a few sidechains in the structure with alternative 
conformations…


Huw 
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