Hi,
This is really 2 questions, one related to the other coming from someone who is just starting to learn/use CCP4/COOT so this question might sound rather naive. Q1) I have a MTZ file and I want to generate a another MTZ from it but limited to the backbone atoms only. (Similar to FFT with all atoms in PDB file) but I need a MTZ file since I want to use the map sharpening tool (B factor attenuation) in COOT. I tried using REFMAC with the input PDB only having the backbone atoms but it fails due to the inconsistency between the input MTZ and input PDB. Any other way I can generate a truncated MTZ? Q2) Once I use the map sharpen tool, could I save (as MTZ ) the new sharpened map? Thank you. George
