On Thu, May 29, 2014 at 3:17 PM, George Devaniranjan <[email protected] > wrote:
> Thank you for your reply, Dale. > Let me rephrase the question: > Essentially I want to use COOT's Calculate-->map sharpening > but only use the backbone atoms in the process. > Perhaps you want an omit map? In that case, your attempt to generate a map using Refmac and a backbone-only model is the correct approach, so you should figure out why that isn't working. (I suspect the problem is that the PDB file is missing the symmetry information, i.e. the CRYST1 record.) But that won't magically get rid of the sidechain density - they just won't be as clear because the phases will be much worse. The experimental amplitudes are still the same. -Nat
