Hi folks
I am working on a CryoEM map of a ribosome (specifically a 70S .mrc from Relion, looking at both the 3Drefined map focus-refined with a mask on the 30S and the corresponding Relion postprocessed map with the same mask). My aim is evaluating which parts of a ligand´s density appear at lower/higher threshold. In the field, the sigma is used (The ligand is visible at X sigma etc). Coot shows RMSD while scrolling. I did some homework and it seems to me that a certain value Y rmsd in Coot is basically equivalent to the corresponding X sigma. However, before publishing this and writing it down, I wanted your opinion if effectively X and Y are the same, and if not how to understand their correlation. I am asking because I am not sure whether the way I processed these maps may affect the X/Y correlation. Thank you so much - M. ######################################################################## To unsubscribe from the COOT list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=COOT&A=1 This message was issued to members of www.jiscmail.ac.uk/COOT, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
