Yes, these two volume are aligned. But they are sliced differently. When you display a single volume alone in freeview, it is by default displayed in its native slice orientation.

Ruopeng

On 9/16/19 3:39 PM, Alexopoulos, Dimitrios wrote:

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I’m still a little confused. When I open up the brainmask.mgz in freeview I see the following. It is rotated compared to the native 001.mgz below.

I then open the 001.mgz with freeview in a NEW terminal (seperate session),  I get the following.  Should thy not be the same orientation?

*From:*freesurfer-boun...@nmr.mgh.harvard.edu <freesurfer-boun...@nmr.mgh.harvard.edu> *On Behalf Of *Ruopeng Wang
*Sent:* Monday, September 16, 2019 1:17 PM
*To:* freesurfer@nmr.mgh.harvard.edu
*Subject:* Re: [Freesurfer] 001 vs brainmask views in freeview

The first volume is always displayed in its original orientation where pixel data are stored. So that the pixel data don't get resampled (unless you force it with '-r' flag to resample it in standard RAS).

Ruopeng

On 9/16/19 1:06 PM, Alexopoulos, Dimitrios wrote:

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    Thanks for info.

    If I open only the brainmsk.mgz only from a new terminal it is
    rotated and not in its native orientation (which is 001’s
    orientation).

    Why does it not appear to be in native space in freeview?

    Jim

    *From:*freesurfer-boun...@nmr.mgh.harvard.edu
    <mailto:freesurfer-boun...@nmr.mgh.harvard.edu>
    <freesurfer-boun...@nmr.mgh.harvard.edu>
    <mailto:freesurfer-boun...@nmr.mgh.harvard.edu> *On Behalf Of
    *Ruopeng Wang
    *Sent:* Monday, September 16, 2019 11:51 AM
    *To:* freesurfer@nmr.mgh.harvard.edu
    <mailto:freesurfer@nmr.mgh.harvard.edu>
    *Subject:* Re: [Freesurfer] 001 vs brainmask views in freeview

    Hi,

    When you load brainmask.mgz as the second volume, it will be
    realigned to 001.mgz. The default resample method is
    nearest-neighbor. Thus you get the blocky look. You can try
    loading it with trilinear or cubic resampling like this from the
    command-line:

    freeview -v 001.mgz brainmask.mgz -trilinear

    Ruopeng

    On 9/16/19 12:41 PM, Alexopoulos, Dimitrios wrote:

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        Hi all,

        I ran a 2yo subject through FS6.0 using ‘recon-all -i
        /firstdicom.dcm -all’ and the output segmentations and
        surfaces looks good.

        Can someone explain why I see different alignments when I view
        the 001.mgz and brainmask.mgz files in freeview, depending on
        the order files are opened.

        001.mgz AND brainmask.mgz -- 001 opened first in freeview
        followed by brainmask.mgz. Alignemnts OK but brainmask looks
        grainy.

        Brainmask.mgz opened only in a new terminal. Rotated and looks
        smoother than brainmask above.

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