> I have shared my hisotry with you yesterday, can you help me to take a look to
> see what is the problem for my tophat analysis? Thank you very much!
Unfortunately, your job is too large for our cluster. Our cluster has a limit
of 250GB of disk space, and Tophat is exceeding this limit while running your
job (Tophat generates many intermediary files when running, including a very
large SAM file).
Your best bet is to set up and use our cloud instance (
https://bitbucket.org/galaxy/galaxy-central/wiki/cloud ); otherwise, if you
have enough resources locally, you can set up a local instance (
https://bitbucket.org/galaxy/galaxy-central/wiki/GetGalaxy ) .
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