> I have shared my hisotry with you yesterday, can you help me to take a look to
> see what is the problem for my tophat analysis? Thank you very much!


Unfortunately, your job is too large for our cluster. Our cluster has a limit 
of 250GB of disk space, and Tophat is exceeding this limit while running your 
job (Tophat generates many intermediary files when running, including a very 
large SAM file). 

Your best bet is to set up and use our cloud instance ( 
https://bitbucket.org/galaxy/galaxy-central/wiki/cloud ); otherwise, if you 
have enough resources locally, you can set up a local instance ( 
https://bitbucket.org/galaxy/galaxy-central/wiki/GetGalaxy ) .

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