On Tue, May 24, 2011 at 12:40 AM, Aaron Jex <a...@unimelb.edu.au> wrote: > Hi, > > Can’t seem to find an answer to this on your wiki site and it’s not in the > tutorial. I would like to filter my 454 reads for high quality regions, > rename the resulting sequence fragments AND relink the new reads (fragments) > to the original quality data so that I can take these filtered reads and > assembly them using MIRA. Is there a way to do this with Galaxy?
See Alex's answer. > So > basically all I want to do is take the new read fragments I get from > converting the tabular file to the fasta file as shown in your metagenomics > tutorial, and generate a corresponding qual file for these ‘new’ reads. When working in Galaxy, I use the SFF converter tool to make FASTQ rather than FASTA and QUAL. MIRA will also read in FASTQ, and I find that is easier to work with for filtering and trimming than a matched FASTA and QUAL file. Peter ___________________________________________________________ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using "reply all" in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/