Barbie Heid wrote:
Reptile CBCs are also done using the unopet manual method - requires 0.25 cc total volume.That means that all one may take is approximately 0.37 cc of blood.
Tough to do much with that - but you might get a cbc and a chem or two.
I am not saying it cannot be done, but even acquiring the sample will
be, lets say, challenging.
From the obsessive/compulsive lab person :)
Oooof, that'd be pushing it. We've pulled off cbc's from even the worst fingersticks, but chem's, by the time you spin it down and pull the serum off ... :(
Yep - there are some labs that dulirte the sample and back calculate - but the error induced generally renders the results clinically irrelevant.Unfortunately, almost all automated analyzers have a minimum amount of serum required.
It is generally pretty darn low - most herps are.Depending on how high a gecko's hemoglobin (red cells) is, you may wind up with just a clot and a few drops of serum.
Yep - and one would likey use an insulin syringe.Plus, the finer the needle, the higher the chances you're going to get a hemolyzed sample;
the red cells actually break going through the needle,I hate that. They break coming back out too - when shot into a blood collection tube. I hate that too.
which will give you false critical values, especially in glucose andSo far the only herp looked at was a bit different than humans with respect to glucose and some other cytosolic constituents. But the principle is the same. Stuff found in greater quiantity inside a RBC will increase in a hemolyzed sample if th said red cells are broken. For details see:
potassium.
http://www.anapsid.org/hemolysis.html
Keith
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