Hi Tapas, Whether you obtain the DNA sequence through our downloads page or the "DNA" button in the blue bar when you are viewing an assembly, it always defaults to the positive strand. The easiest way to get DNA for a short sequence, such as the one you mentioned in your email, is to use the "get DNA" feature (http://genome.ucsc.edu/cgi-bin/hgc?g=getDna). Simply type in the coordinates and click the "get DNA" button. Note that you can also get DNA from the negative strand by clicking the "reverse complement" check box.
I hope this answers this question and both of your questions that were emailed to our team yesterday. Please feel free to contact the mail list again if you require further assistance. Best, Mary ------------------ Mary Goldman UCSC Bioinformatics Group On 6/24/10 4:33 AM, Tapas Bhadra wrote: > Dear Sir, > I am a research fellow at the Indian Statistical Institute, Kolkata, > India. > > I have downloaded the sequences of all the chromosomes. Can you please > inform me that if I need sequences from 1000bp to 1500bp of chr 6 from +ve > strand and also from 1000bp to 1500bp of chr 6 from -ve strand, then how > will I obtain those information. Will they be same? > > Is your database strand specific? If yes then which strand it annotates > for the sequence and for this strand specific database how will we obtain > sequential information from the reverse strand? > > Pleaes reply at your earliest convenience. > > Thanking you, > Tapas Bhadra, > Senior Research Fellow, > Indian Statistical Institute, Kolkata, > India > This mail is scanned by Ironport > > > _______________________________________________ > Genome maillist - [email protected] > https://lists.soe.ucsc.edu/mailman/listinfo/genome > _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
