Hi Tapas,

My apologies - the browser is looking for default values to enter into 
the position text box. The link below should work for hg18, chr6:1000-1500:

http://genome.ucsc.edu/cgi-bin/hgc?g=getDna&i=mixed&c=chr6&l=999&r=1500&db=hg18

Best,
Mary
---------------------
Mary Goldman
UCSC Bioinformatics Group

On 6/24/10 4:57 PM, Tapas Bhadra wrote:
> Sir,
>      Thanks for your reply. But when I am trying to go into the site it
> shows that "hashMustFindVal: 'c' not found " in that page.
>
>     Pleaes reply at your earliest convenience.
>
>     Thanking you,
>                                         Tapas Bhadra,
>                                         Senior Research Fellow,
>                                         Indian Statistical Institute,
>                                         Kolkata,
>                                         India
>
>
>    
>> Hi Tapas,
>>
>> Whether you obtain the DNA sequence through our downloads page or the
>> "DNA" button in the blue bar when you are viewing an assembly, it always
>> defaults to the positive strand. The easiest way to get DNA for a short
>> sequence, such as the one you mentioned in your email, is to use the
>> "get DNA" feature (http://genome.ucsc.edu/cgi-bin/hgc?g=getDna). Simply
>> type in the coordinates and click the "get DNA" button. Note that you
>> can also get DNA from the negative strand by clicking the "reverse
>> complement" check box.
>>
>> I hope this answers this question and both of your questions that were
>> emailed to our team yesterday. Please feel free to contact the mail list
>> again if you require further assistance.
>>
>> Best,
>> Mary
>> ------------------
>> Mary Goldman
>> UCSC Bioinformatics Group
>>
>> On 6/24/10 4:33 AM, Tapas Bhadra wrote:
>>      
>>> Dear Sir,
>>>      I am a research fellow at the Indian Statistical Institute, Kolkata,
>>> India.
>>>
>>> I have downloaded the sequences of all the chromosomes. Can you please
>>> inform me that if I need sequences from 1000bp to 1500bp of chr 6 from
>>> +ve
>>> strand and also from 1000bp to 1500bp of chr 6 from -ve strand, then how
>>> will I obtain those information. Will they be same?
>>>
>>> Is your database strand specific? If yes then which strand it annotates
>>> for the sequence and for this strand specific database how will we
>>> obtain
>>> sequential information from the reverse strand?
>>>
>>> Pleaes reply at your earliest convenience.
>>>
>>>     Thanking you,
>>>                                        Tapas Bhadra,
>>>                                        Senior Research Fellow,
>>>                                        Indian Statistical Institute,
>>> Kolkata,
>>>                                        India
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>>>
>>>
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>>
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