I am having the same problem as Helder in a thread from last spring. I  
have a Centos 5.2 box with 4GB of ram, but when I try to BLAT a small  
sequence:

        CATGCCATCCTGCGTCTGGACCTGGCTGGCZGGGACCTGACTGACTACCTCATGAAGATCC   (Z is  
the muation target)

I get the out of memory error.

        needLargeMem: Out of memory - request size 154913755 bytes

Shouldn't this work on a 4GB memory machine? Is the only solution to  
write a loop in the bash script to check one chromosome at a time?

One other thing. How do I get a 120mer output? I am inputting 60mer  
sequences and need to extend them to 120.

-------------
Doug Wolfgram
[email protected]



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