Hi Hiram,

I am sorry I am still not clear. What I meant was would the 
KNETFILEHOOKS-recompiled samtool work with the old local mirror, or we 
have to recompile the local mirror as well? The reason is recompiling 
the mirror will take quite a bit of time, and we need to contact our 
local admin to do that.

Thanks,

D.

On 11/22/10 4:47 PM, Hiram Clawson wrote:
> You can use the scripts in the source tree: src/product/scripts/
> to build your kent source tree.
>
>
> ----- Original Message -----
> From: "Duke"<[email protected]>
> To: "Angie Hinrichs"<[email protected]>
> Cc: [email protected], "Hiram Clawson"<[email protected]>
> Sent: Monday, November 22, 2010 1:35:23 PM GMT -08:00 US/Canada Pacific
> Subject: Re: [Genome] empty data for BAM tracks
>
> Hi Angie,
>
> No, I dont use KNETFILE_HOOKS and I believe our local mirror was not
> built with that option as well. But our samtools and mirror have been
> working just fine until I encouter that issue. I am gonna apply that
> patch and rebuild our samtools, but does that mean we have to ask our
> local admin to recompile (or apply batch) to the genome code as well?
>
> Thanks,
>
> D.
>
>
> On 11/22/10 11:43 AM, Angie Hinrichs wrote:
>> Hi Duke,
>>
>> Did you compile your samtools and kent/src with KNETFILE_HOOKS=1 ?  (That 
>> requires downloading and applying a patch to the samtools source, see 
>> http://genomewiki.ucsc.edu/index.php/KNETFILE_HOOKS .)  If so, is it 
>> possible to make a small test file that has the same problem, and put it on 
>> a public site or email to me for debugging?
>>
>> If you didn't use KNETFILE_HOOKS, then empty bam track can be caused by 
>> stale index files (.bam.bai) that the samtools C library saves in a local 
>> directory.  To clear all index files, "rm ${TRASHDIR}/samtools/*.bai" .  
>> Depending on your installation, TRASHDIR is probably something like 
>> /usr/local/apache/trash .  If the track is still blank after clearing the 
>> index files, please send a test case, or try "samtools view my.bam chr1:2-3" 
>> where chr1:2-3 is the region you are viewing in the browser.
>>
>> Hope that helps,
>> Angie
>>
>> ----- "Duke"<[email protected]>   wrote:
>>
>>> From: "Duke"<[email protected]>
>>> To: "Hiram Clawson"<[email protected]>
>>> Cc: [email protected]
>>> Sent: Saturday, November 20, 2010 6:08:56 AM GMT -08:00 US/Canada Pacific
>>> Subject: Re: [Genome] empty data for BAM tracks
>>>
>>> On 11/19/10 8:33 PM, Hiram Clawson wrote:
>>>> Take a look in your Apache error log files to see if there
>>>> are any error messages about your track from the browser CGI
>>>> binaries.  The messages should have appeared when you loaded
>>>> the tracks, but maybe something else is going wrong.
>>> Thanks Hiram for your suggestion. I have contacted our local admin about
>>> that. Aside from the admin side, based on your experience, is there any
>>> other reason from the user side that can cause the issue?
>>>
>>> Thanks,
>>>
>>> D.
>>>
>

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