i've uploaded myTest.bam, myTest.bam.bai and myTest.bw to an other external web server and everything is fine, thanks a lot for that Galt ! i did not found any configuration line type "RequestHeader unset Range" but i guess it can be also a problem due to the firewall of the institute. I'm going to try to solve this today. Thanks again. kevin.
2011-02-10 Kevin LEBRIGAND De : Galt Barber Date/heure : 2011-02-10 11:04:10 A : Kevin LEBRIGAND Cc : genome Sujet : Re: [Genome] BAM file visualisation By the way, we have added code to detect this problem earlier and have improved the error message to explain the problem better. -Galt 2/10/2011 12:04 AM, Galt Barber: > > Hi, Kevin! > > It looks like your apache says it will accept byte-range requests, > i.e. it includes this in the response header when you query the server: > Accept-Ranges: bytes > > But when we actually do send the range request, your server ignores it > and just sends back the entire file, and 200 instead of 206 partial content. > > My guess is that you have a line like this somewhere in your apache > configuration that should be considered for removal: > > RequestHeader unset Range > > If that line exists in your configuration, it will strip out > all incoming range requests. > > Looking at apache history, between version 2.0 and 2.0.54, > there was a denial of service bug that affected reverse-proxy > which could allow attackers to cause the server to waste ram. > > Since you now have version 2.2, that apache bug is irrelevant > and you should be safe to allow range requests. > > bigDataUrl in custom tracks will not work without range requests. > > -Galt > > 2/9/2011 9:43 AM, Kevin LEBRIGAND: >> Sorry for that. >> I've restored the file >> here is the custom track i paste in UCSC custom tracks: >> track type=bam name="myTest" description="miRNAs hg19" db=hg19 >> visibility=pack >> bigDataUrl=http://www.microarray.fr/data/public/tmp/myTest.bam >> browser position chr17:57,918,627-57,918,698 >> >> Samtools view at this position returns 142322 reads but i see nothing in >> UCSC web browser. >> >> I've a second question maybe from the same issue: >> i've tryed to visualize .bw file generated from my .bam file as follow: >> $> genomeCoverageBed -bg -ibam myTest.bam -g hg19.chrsize> >> myTest.bedGraph >> $> bedGraphToBigWig myTest.bedGraph hg19.chrsize myTest.bw >> >> UCSC custom tracks: >> track name="Wiggle Track" type=bigWig >> bigDataUrl=http://myserver.edu/sample.bw >> >> I got an error: >> http://www.microarray.fr/data/public/tmp/myTest.bw is not a chromosome id >> r-tree index file >> >> thanks angie for your help. >> best >> kevin. >> >> >> >> >> >> Kevin LEBRIGAND >> 2011-02-09 >> _______________________________________________ >> Genome maillist - [email protected] >> https://lists.soe.ucsc.edu/mailman/listinfo/genome > > _______________________________________________ > Genome maillist - [email protected] > https://lists.soe.ucsc.edu/mailman/listinfo/genome _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
