I am trying to use BLAT to blast short reads(FASTQ) from the NCBI, SRA with my 
custom reference sequence(fasta format) to check the hits and then design 
primers. I am bit confused how to use the program efficiently. I am planning to 
read the help to find solutiosn. I would really appreciate, if you can help me 
with the above situation.

Thank you,
Shakuntala Fathepure
Postdoc
Dept. of Botany
Oklahoma State University
Stillwater, OK 74078
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