If you are only doing one phase encode direction, I would strongly recommend 
doing p>a. It has a large number of advantages particularly in the frontal 
lobes.

hth
D

Sent from my Phone

> On Dec 8, 2016, at 16:56, neuroimage analyst <[email protected]> 
> wrote:
> 
> A>>P
> 
> Thanks
> 
> Regards
> 
> --VM
> 
>> On Thu, Dec 8, 2016 at 1:40 PM, Glasser, Matthew <[email protected]> wrote:
>> What phase encoding directions are you using?
>> 
>> Peace,
>> 
>> Matt.
>> 
>> From: neuroimage analyst <[email protected]>
>> Date: Thursday, December 8, 2016 at 1:37 PM
>> To: "Harms, Michael" <[email protected]>
>> Cc: Matt Glasser <[email protected]>, "[email protected]" 
>> <[email protected]>
>> Subject: Re: [HCP-Users] FW: Skyra - BOLD and diffusion MRI protocols
>> 
>> Hi, 
>> 
>> Just an update:
>> 
>> 1) BOLD: I was able to run the sequence with all the parameters as mentioned 
>> but with the resolution of 2.5mm3 and Partial fourier 7/8. I was unable to 
>> decrease the resolution with that Partial Fourier factor of anything or 
>> resolution of 2.5mm3 with no partial fourier. I will scan a subject today 
>> and see how the data looks.
>> 
>> 2) dMRI: MB factor = 3. RF pulse clipping issue was resolved. TR is 5600 and 
>> TE is 100 ms , 2 b-values and 71 diffusion encoding directions running under 
>> free mode. Resolution of 1.5mm3; scan time of 15 mins. I added a PA phase 
>> encoding with 2 averages of b0. Again, I will test the sequence with a 
>> subject and different resolutions to see if the data is acceptable.
>> 
>> Thank you for all your help and advice/suggestions.
>> 
>> Regards
>> 
>> --VM
>> 
>>> On Wed, Dec 7, 2016 at 12:31 PM, Harms, Michael <[email protected]> wrote:
>>> 
>>> Oh.  I read too quickly and conflated the problem you were having for the 
>>> BOLD with the dMRI.  So, you can set up a BOLD scan with those parameters, 
>>> and it only fails at run-time?  Can you achieve a higher MB factor if you 
>>> relax the spatial resolution to say 2.4 mm.  (Although that shouldn’t be 
>>> necessary, because it is possible to do 2.0 mm, MB=8 BOLD on a Trio).  You 
>>> might have to ask the CMRR folks via their github issues page:
>>> https://github.com/CMRR-C2P/MB/issues
>>> 
>>> Regarding the dMRI:  What MB factor were you using there?   And did you 
>>> resolve the issue with the RF pulse clipping?   (Either by lengthening the 
>>> pulses further (within reason), or you could try using the “MB RF phase 
>>> scramble” option; and if that isn’t sufficient, try the “Time-shifted MB 
>>> RF” option as well).
>>> 
>>> cheers,
>>> -MH
>>> 
>>> -- 
>>> Michael Harms, Ph.D.
>>> -----------------------------------------------------------
>>> Conte Center for the Neuroscience of Mental Disorders
>>> Washington University School of Medicine
>>> Department of Psychiatry, Box 8134
>>> 660 South Euclid Ave.Tel: 314-747-6173
>>> St. Louis, MO  63110Email: [email protected]
>>> 
>>> From: neuroimage analyst <[email protected]>
>>> Date: Wednesday, December 7, 2016 at 1:54 PM
>>> To: Michael Harms <[email protected]>
>>> Cc: "Glasser, Matthew" <[email protected]>, 
>>> "[email protected]" <[email protected]>
>>> Subject: Re: [HCP-Users] FW: Skyra - BOLD and diffusion MRI protocols
>>> 
>>> I apologize for not making the question very clear. I think I have sort of 
>>> optimized the diffusion protocol the way I wanted it to be. I just need to 
>>> do a human scan and compare SNR between different resolutions to see the 
>>> limit where i can get acceptable data.
>>> 
>>> The issue that I am unable to solve is this:
>>> BOLD: We were hoping to get a TR in the range of 750-900ms, res = 2mm3, 
>>> echo spacing (ES) approx 0.65. We were able to get to echo spacing of 0.69 
>>> and all the other parameters with MB = 8. However, the sequence doesn't run 
>>> and it gives us "Max amplitude overflow on gradient z axis" after MB factor 
>>> exceeds 2.  It appears to me that then we have to sacrifice TR and only run 
>>> with MB = 2. We are using 32 channel head coil.
>>> 
>>> Thank you for any suggestions.
>>> 
>>> Regards
>>> 
>>> Virendra
>>> 
>>>> On Wed, Dec 7, 2016 at 11:22 AM, Harms, Michael <[email protected]> wrote:
>>>> 
>>>> I wouldn’t think that increasing the MB factor would increase the gradient 
>>>> requirements that much, so I’m wondering if trying to run with b_max = 
>>>> 2500 isn’t at the very edge of what can be achieved on the Skyra for that 
>>>> particular gradient direction set.  What happens if you lower the b_max to 
>>>> say 1500?  Can you then increase the MB factor without getting that error?
>>>> 
>>>> -- 
>>>> Michael Harms, Ph.D.
>>>> -----------------------------------------------------------
>>>> Conte Center for the Neuroscience of Mental Disorders
>>>> Washington University School of Medicine
>>>> Department of Psychiatry, Box 8134
>>>> 660 South Euclid Ave.Tel: 314-747-6173
>>>> St. Louis, MO  63110Email: [email protected]
>>>> 
>>>> From: neuroimage analyst <[email protected]>
>>>> Date: Wednesday, December 7, 2016 at 1:03 PM
>>>> To: Michael Harms <[email protected]>
>>>> Cc: "Glasser, Matthew" <[email protected]>, 
>>>> "[email protected]" <[email protected]>
>>>> Subject: Re: [HCP-Users] FW: Skyra - BOLD and diffusion MRI protocols
>>>> 
>>>> Thanks for that reply, Micahel. Exactly, what I plan to do for diffusion 
>>>> protocol.
>>>> 
>>>> Just wondering though on your or anyone else's suggestion on running BOLD 
>>>> with MB>2.
>>>> 
>>>> Thanks
>>>> 
>>>> Regards
>>>> 
>>>> --VM
>>>> 
>>>> 
>>>>> On Wed, Dec 7, 2016 at 10:50 AM, Harms, Michael <[email protected]> wrote:
>>>>> 
>>>>> Hi,
>>>>> It appears that you are trying to replicate the basics of one of our 
>>>>> Lifespan pilot protocols on a Skyra — i.e., 1.5 mm voxels with 20 total 
>>>>> min of dMRI scanning.  But due to the longer TE and longer TR (resulting 
>>>>> in fewer total directions), you are definitely going to have worse 
>>>>> overall SNR than what we achieved.  We highly suggest that you collect 
>>>>> some pilot data to assess the quality of your proposed dMRI protocol with 
>>>>> your end-measures of interest, including possibly a comparison against a 
>>>>> protocol with larger voxels (e.g., 1.7-1.8 mm voxels) that would recover 
>>>>> some SNR.
>>>>> 
>>>>> cheers,
>>>>> -MH
>>>>> 
>>>>> -- 
>>>>> Michael Harms, Ph.D.
>>>>> -----------------------------------------------------------
>>>>> Conte Center for the Neuroscience of Mental Disorders
>>>>> Washington University School of Medicine
>>>>> Department of Psychiatry, Box 8134
>>>>> 660 South Euclid Ave.Tel: 314-747-6173
>>>>> St. Louis, MO  63110Email: [email protected]
>>>>> 
>>>>> From: <[email protected]> on behalf of "Glasser, 
>>>>> Matthew" <[email protected]>
>>>>> Date: Wednesday, December 7, 2016 at 11:49 AM
>>>>> To: "[email protected]" <[email protected]>
>>>>> Subject: [HCP-Users] FW: Skyra - BOLD and diffusion MRI protocols
>>>>> 
>>>>> 
>>>>> 
>>>>> From: Matt Glasser <[email protected]>
>>>>> Date: Wednesday, December 7, 2016 at 10:14 AM
>>>>> To: neuroimage analyst <[email protected]>
>>>>> Subject: Re: [HCP-Users] Skyra - BOLD and diffusion MRI protocols
>>>>> 
>>>>> I wouldn’t acquire multiple repetitions of the same directions, but 
>>>>> rather more unique directions.  If necessary you can acquire the DWIs 
>>>>> with only a single phase encoding direction in an effort to get more 
>>>>> unique diffusion directions, but it is important that you get phase 
>>>>> reversed b0 images.  
>>>>> 
>>>>> As for the MB error, I don’t know how to fix it, but you should 
>>>>> definitely be able to do better than MB=2 with a 32 channel head coil.  
>>>>> Hopefully someone else on the list knows how to fix this.  
>>>>> 
>>>>> Peace,
>>>>> 
>>>>> Matt.
>>>>> 
>>>>> From: neuroimage analyst <[email protected]>
>>>>> Date: Tuesday, December 6, 2016 at 8:54 PM
>>>>> To: Matt Glasser <[email protected]>
>>>>> Subject: Re: [HCP-Users] Skyra - BOLD and diffusion MRI protocols
>>>>> 
>>>>> Hi Matt,
>>>>> 
>>>>> 32 channel head coil.
>>>>> 
>>>>> I was able to run the diffusion sequence on a phantom when TR/TE was 
>>>>> 7000/106 ms. Since the scan time with this tr is already 20 minutes, I 
>>>>> was thinking to acquire just b0 with PA phase encoding and the other 64 
>>>>> directions 2b data+b0  with AP. will that be okay? my concern is snr at 
>>>>> te will not be very good and data may not be too great and may be atleast 
>>>>> 2 reps are desired?
>>>>> 
>>>>> Thanks
>>>>> 
>>>>> Regards 
>>>>> 
>>>>> -VM
>>>>> 
>>>>>> On Dec 6, 2016 6:46 PM, "Glasser, Matthew" <[email protected]> wrote:
>>>>>> What coil are you using?
>>>>>> 
>>>>>> Peace,
>>>>>> 
>>>>>> Matt.
>>>>>> 
>>>>>> From: <[email protected]> on behalf of neuroimage 
>>>>>> analyst <[email protected]>
>>>>>> Date: Tuesday, December 6, 2016 at 11:56 AM
>>>>>> To: "[email protected]" <[email protected]>
>>>>>> Subject: [HCP-Users] Skyra - BOLD and diffusion MRI protocols
>>>>>> 
>>>>>> Hi, HCP Users and Developers.
>>>>>> 
>>>>>> We have been trying to build a protocol for BOLD and DWI on our Skyra 
>>>>>> (VE11C) using the latest release of HCP pulse sequences. But we haven't 
>>>>>> been completely successful with what we wanted to achieve.
>>>>>> 
>>>>>> A) BOLD: We were hoping to get a TR in the range of 750ms, res = 2mm3, 
>>>>>> echo spacing (ES) approx 0.65. We were able to get to echo spacing of 
>>>>>> 0.69 and all the other parameters with MB = 8. However, the sequence 
>>>>>> doesn't run and it gives us "Max amplitude overflow on gradient z axis" 
>>>>>> after MB factor exceeds 2.  It appears to me that then we have to 
>>>>>> sacrifice TR and only run with MB = 2. Is there anybody with a Skyra who 
>>>>>> has able to achieve what we are hoping for and willing to share the 
>>>>>> protocol with us? OR if somebody could guide us to resolve the error of 
>>>>>> amplitude overflow.
>>>>>> 
>>>>>> B) DWI: The idea was to have res = 1.5mm3, 2b-values at 1000 and 2500, 
>>>>>> 64 directions with the best BW and ES achievable. The sequence runs for 
>>>>>> 7 minutes out of total 13 minutes and gives gradient power amplifier 
>>>>>> error. On Michael Harms's suggestion, I adjusted flip angle to 78/160 at 
>>>>>> a TR of 5500 ms with excite/refocus pulse duration of 3840 and 7680. 
>>>>>> there was a pop up warning that RF is clipped and the maximum refocusing 
>>>>>> angle was 142 instead of 160. I placed a 64 directions diffusion vector 
>>>>>> and ran the sequence under "free" mode. Again, I will appreciate if  
>>>>>> there is anybody with a Skyra who has able to achieve what we are hoping 
>>>>>> for and willing to share the protocol with us, along with the diffusion 
>>>>>> vector sets? OR if somebody could guide us to resolve the error.
>>>>>> 
>>>>>> Thank you.
>>>>>> 
>>>>>> Regards 
>>>>>> 
>>>>>> --VM
>>>>>> 
>>>>>> _______________________________________________
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>>>>>> 
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>>>>> 
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>>>>> 
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>>>> 
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>> in error, please immediately notify the sender via telephone or return mail.
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