Really good discussion i learnt a lot about SEM TEM On Wed, Jan 11, 2012 at 2:06 PM, Gurcharan Singh <[email protected]> wrote:
> Thanks a lot Ushadi > > > -- > Dr. Gurcharan Singh > Retired Associate Professor > SGTB Khalsa College, University of Delhi, Delhi-110007 > Res: 932 Anand Kunj, Vikas Puri, New Delhi-110018. > Phone: 011-25518297 Mob: 9810359089 > http://people.du.ac.in/~singhg45/ > > On Wed, Jan 11, 2012 at 1:25 PM, ushadi Micromini < > [email protected]> wrote: > >> Gurchranji: >> Micromorphology is fine... or Microtopography may be apt... >> just because the new SEM machines can look even closer, like at 300,000 >> magnification, still does not make it look at thin sections and the inside >> cytoplasm etc... Whatever is on the surface, including surface of >> whatever is inside cells or sacks etc that can be fractured open is looked >> at... >> >> But there is one caveat... if one purifies actin etc of cytoskeletal >> structures of a cell and then plates them, dries them, coats etc processes >> them, one can look at its structure, but that still is the surface >> morphology... of protein or whatever... >> >> Japanese market now has an open chamber SEM where wet tissues without >> processing or drying can be examined... I personally have not seen it, only >> a small brochure... so cant comment on it... >> >> ==== >> usha di >> =========== >> >> >> >> >> >> On Wed, Jan 11, 2012 at 9:54 AM, Gurcharan Singh <[email protected]>wrote: >> >>> Thanks Vijayasankar ji and Pankaj ji for these papers. >>> I have just given final approval to 3rd Indian edition "Plant >>> Systematics, Theory and Practice" due in January 2012 in which I have >>> managed to incorporate three major changes of 18th IBC (changing name from >>> ICBN to ICN, allowing English description or diagnosis for Valid >>> publication and allowing online publication for Effective publication; in >>> addition to single name for each Fungus (no separate form genera) and >>> fossils), as also incorporating Takhtajan (2009) and APG III >>> classifications (which I could not do in 3rd International Edition "Plant >>> Systematics, An Integrated Approach, which had gone out from me in >>> September 2009, and published in January 2010). Wish I had this information >>> (courtesy Ushadi, Pankaj ji and Vijayasankar ji) earlier to incorporate in >>> my chapter on Ultrastructure and micromorphology. >>> Just seeking a suggestion. For last at least three decades or so TEM >>> structures were characterized as ultrastructure and SEM structures as >>> Micromorphology in most books on Plant Systematics. Now that SEM can also >>> explore anatomical features, what could be an appropriate term to cover >>> these studies?. I was thinking about appropriate title for my chapter which >>> is now "Utrastructure and Micromorphology". >>> >>> >>> -- >>> Dr. Gurcharan Singh >>> Retired Associate Professor >>> SGTB Khalsa College, University of Delhi, Delhi-110007 >>> Res: 932 Anand Kunj, Vikas Puri, New Delhi-110018. >>> Phone: 011-25518297 Mob: 9810359089 >>> http://people.du.ac.in/~singhg45/ >>> >>> >>> >>> On Wed, Jan 11, 2012 at 6:16 AM, Dr Pankaj Kumar <[email protected] >>> > wrote: >>> >>>> http://epress.anu.edu.au/wp-content/uploads/2011/05/ch0811.pdf >>>> >>>> Sir you may check this. >>>> Pankaj >>>> >>>> >>>> >>>> On Jan 10, 6:18 pm, Prashant Awale <[email protected]> wrote: >>>> > Dear Vijayasankar ji, >>>> > >>>> > I really liked this post. Its really a "divine feeling." Thanks for >>>> this >>>> > unique post. >>>> > Regards >>>> > Prashant >>>> > On Tue, Jan 10, 2012 at 3:08 PM, Ushadi micromini < >>>> [email protected] >>>> >>>> > >>>> > >>>> > >>>> > >>>> > >>>> > >>>> > >>>> > > wrote: >>>> > > Gurcharanji: >>>> > > I dont mean to correct you... >>>> > > but all are SEM..... >>>> > >>>> > > not a single one of these is a TEM.... >>>> > >>>> > > Usha di >>>> > > ========== >>>> > >>>> > > On Jan 10, 1:52 pm, Gurcharan Singh <[email protected]> wrote: >>>> > > > Thanks Pankaj ji and Ushadi >>>> > > > I read second mail of Vijay ji and somehow missed looking at >>>> attachments. >>>> > > > Thanks for bringing it to my notice. >>>> > >>>> > > > Should we say >>>> > > > First picture is SEM of leaf showing trichomes >>>> > > > Second and third picture are TEM of cells showing crystals >>>> > > > 4th again seems to be SEM >>>> > >>>> > > > -- >>>> > > > Dr. Gurcharan Singh >>>> > > > Retired Associate Professor >>>> > > > SGTB Khalsa College, University of Delhi, Delhi-110007 >>>> > > > Res: 932 Anand Kunj, Vikas Puri, New Delhi-110018. >>>> > > > Phone: 011-25518297 Mob: 9810359089 >>>> http://people.du.ac.in/~singhg45/ >>>> > >>>> > > > On Tue, Jan 10, 2012 at 2:11 PM, Ushadi micromini < >>>> > > [email protected] >>>> > >>>> > > > > wrote: >>>> > >>>> > > > > Gurcharanji: >>>> > > > > if you go to abridged summary that came this morning and go to >>>> the >>>> > > > > first email... its from Raman... and its title is :" My Flora >>>> > > > > Picture of the Year 2011: Vijayasankar Raman " >>>> > >>>> > > > > there you scroll down ... and just above my first comment ... >>>> are the >>>> > > > > SEM pics ... i was asking about... >>>> > >>>> > > > > Is n't it wonderful.... >>>> > > > > ??? >>>> > >>>> > > > > love it >>>> > > > > Usha di >>>> > > > > ============ >>>> > >>>> > > > > On Jan 10, 1:37 pm, ushadi Micromini <[email protected] >>>> > >>>> > > > > wrote: >>>> > > > > > Thanks Pankaj... >>>> > > > > > crystals I could tell... >>>> > >>>> > > > > > My q to Vijaya was / is : is it cell wall... that's so rigid >>>> and >>>> > > forming >>>> > > > > > the rhomboid spongy looking mesh... >>>> > >>>> > > > > > usha di >>>> > > > > > ======== >>>> > >>>> > > > > > On Tue, Jan 10, 2012 at 1:55 PM, Pankaj Kumar < >>>> > > [email protected] >>>> > > > > >wrote: >>>> > >>>> > > > > > > Mam, 2nd and third pics show crystals (raphides) inside the >>>> cell. >>>> > > > > > > These crystals as well as their structure sometimes form a >>>> good >>>> > > > > > > taxonomic character of groups. >>>> > > > > > > Pankaj >>>> > >>>> > > > > > > On Tue, Jan 10, 2012 at 4:19 PM, Ushadi micromini >>>> > > > > > > <[email protected]> wrote: >>>> > > > > > > > Dear Vijayashankar: >>>> > >>>> > > > > > > > A I am so excited ... >>>> > >>>> > > > > > > > 1: first for you, this will add some new or deeper >>>> insights >>>> > > to >>>> > > > > > > > your ongoing studies.... but of course you knew that...! >>>> > > > > > > > 2: I am excited for myself and the whole of eflora >>>> > > family... >>>> > > > > > > > we will get to see the botanical specimen in further >>>> details... >>>> > > as >>>> > > > > and >>>> > > > > > > > when you share your finds... >>>> > >>>> > > > > > > > ================== >>>> > >>>> > > > > > > > B: >>>> > >>>> > > > > > > > Now, you will be Vijyashankar micromini .... >>>> > > > > > > > instead of / in addition to me... >>>> > >>>> > > > > > > > the micromini epithet was attached to my name by a few >>>> friends >>>> > > and >>>> > > > > > > > colleagues a couple or three decades ago because I was >>>> fond of >>>> > > doing >>>> > > > > > > > TEM and SEM of tumors etc....and ribosome...and >>>> mitochondria... >>>> > >>>> > > > > > > > ======== >>>> > >>>> > > > > > > > C: and this the question section :-) >>>> > >>>> > > > > > > > the first pic is surface of leaf..... i imagine.... >>>> > >>>> > > > > > > > but what are 2 and 3rd pictures of ... a fracture of >>>> what? >>>> > >>>> > > > > > > > is it of a leaf and the rhoboid? structures cell walls... >>>> with >>>> > > > > > > > crystals etc inside? >>>> > >>>> > > > > > > > ============== >>>> > >>>> > > > > > > > just cant stop smiling.... what a great new dimension >>>> to our >>>> > > > > > > > interaction will this make.... >>>> > > > > > > > wonderful for all of us... >>>> > >>>> > > > > > > > and congratulations for learning the techniques... >>>> > > > > > > > ushadi >>>> > >>>> > > > > > > > ========== >>>> > > > > > > > ps does this grow in mississipi??? >>>> > >>>> > > > > > > > ================= >>>> > >>>> > > > > > > > On Jan 10, 12:44 pm, "J.M. Garg" <[email protected]> >>>> wrote: >>>> > > > > > > >> Thanks, Vijayasankar ji, >>>> > > > > > > >> Wonderly different idea & picture. >>>> > >>>> > > > > > > >> For others: >>>> > > > > > > >> Scanning Electron Microscopy (SEM): >>>> > >>>> > > >>>> http://serc.carleton.edu/research_education/geochemsheets/techniques/. >>>> > > > > .. >>>> > >>>> > > > > > > >> A link for Scanning electron microscope: >>>> > > > > > >http://en.wikipedia.org/wiki/Scanning_electron_microscope >>>> > >>>> > > > > > > >> On 10 January 2012 05:15, Vijayasankar < >>>> > > [email protected]> >>>> > > > > > > wrote: >>>> > >>>> > > > > > > >> > Dear all >>>> > >>>> > > > > > > >> > Here is yet another b&w picture as 'my flora picture >>>> of the >>>> > > year >>>> > > > > > > 2011'. >>>> > > > > > > >> > I am not sure if this is suitable for this title, >>>> though. >>>> > >>>> > > > > > > >> > But this is my first ever SEM picture :) It was my >>>> > > > > dream....finally >>>> > > > > > > came >>>> > > > > > > >> > true! It was a great experience to learn the techniques >>>> > > without a >>>> > > > > > > tutor >>>> > > > > > > >> > (but now I am helping many...). >>>> > > > > > > >> > The picture shows glandular and non-glandular >>>> trichomes from >>>> > > leaf >>>> > > > > of >>>> > > > > > > >> > Salvia divinorum, a psychoactive plant endemic to >>>> Mexico. >>>> > > > > > > >> > I recorded 5 types and 33 sub-types of trichomes (some >>>> of them >>>> > > > > are new >>>> > > > > > > >> > reports), from a single leaf, thanks to SEM... >>>> > >>>> > > > > > > >> > Regards >>>> > >>>> > > > > > > >> > Vijayasankar Raman >>>> > > > > > > >> > National Center for Natural Products Research >>>> > > > > > > >> > University of Mississippi >>>> > >>>> > > > > > > >> -- >>>> > > > > > > >> With regards, >>>> > > > > > > >> J.M.Garg ([email protected]) >>>> > > > >http://en.wikipedia.org/wiki/User:Jmgarg1 >>>> > > > > > > >> 'Creating awareness of Indian Flora & Fauna' >>>> > > > > > > >> The whole world uses my Image Resource of more than a >>>> *thousand >>>> > > > > > > species* & >>>> > > > > > > >> eight thousand images of Birds, Butterflies, Plants etc. >>>> > > (arranged >>>> > > > > > > >> alphabetically & place-wise): >>>> > > > > > >http://commons.wikimedia.org/wiki/Category:J.M.Garg. You >>>> can also >>>> > > use >>>> > > > > them >>>> > > > > > > >> for free as per Creative Commons license attached with >>>> each >>>> > > image. >>>> > > > > > > >> For identification, learning, discussion & documentation >>>> of >>>> > > Indian >>>> > > > > > > Flora, >>>> > > > > > > >> please visit/ join our Efloraofindia Google e-group: >>>> > > > > > > >>>> http://groups.google.co.in/group/indiantreepix(morethan1760members& >>>> > > > > > > >> 1,00,000 messages on 21/12/11) or Efloraofindia website: >>>> > > > > > > >>>> https://sites.google.com/site/efloraofindia/(withaspeciesdatabase >>>> > > > > > > >> of more than 6000 species). >>>> > > > > > > >> Also author of 'A Photoguide to the Birds of Kolkata & >>>> Common >>>> > > Birds >>>> > > > > of >>>> > > > > > > >> India'. >>>> > >>>> > > > > > > -- >>>> > >>>> > > >>>> ********************************************************************** >>>> > > > > > > "Taxonomists getting Extinct and Species Data Deficient !!" >>>> > >>>> > > > > > > Pankaj Kumar Ph.D. (Orchidaceae) >>>> > > > > > > Conservation Officer >>>> > >>>> > > > > > > Office: >>>> > > > > > > Orchid Conservation Section >>>> > > > > > > Flora Conservation Department >>>> > > > > > > Kadoorie Farm and Botanic Garden (KFBG) Corporation >>>> > > > > > > Lam Kam Road, Tai Po, New Territories, Hong Kong. >>>> > >>>> > > > > > > Residence: >>>> > > > > > > 36c, Ng Tung Chai, Lam Tseun >>>> > > > > > > Tai Po, New Territories, Hong Kong. >>>> > >>>> > > > > > > email: [email protected] >>>> > > > > > > [email protected] >>>> > > > > > > [email protected] >>>> > > > > > > Phone:+852 2483 >>>> 7128begin_of_the_skype_highlighting +852 2483 7128 (office >>>> - 8:30am to 5:30pm) >>>> > > > > > > +852 9436 >>>> 6251begin_of_the_skype_highlighting +852 9436 6251 (mobile) >>>> > >>>> > > > > > -- >>>> > > > > > Usha di >>>> > > > > > =========== >>> >>> >>> >>> >>> >> >> >> -- >> Usha di >> =========== >> >> > > > -- Regards Dr Balkar Singh Head, Deptt. of Botany and Biotechnology Arya P G College, Panipat Haryana-132103 09416262964
