Amen, lets end it here till Vijaya or may be Tanay shows us some new stuff...
AND calling all those bosses in Botany dept in Indian Universities where TEM AND SEM MACHINES ARE COLLECTING DUST....LET YOUR JUNIORS .... AND GRAD STUDENTS USE THEM.... OTHERWISE SHAME ON YOU..... i WONDER WHAT DOES THE UGC GRANT COMMISSION DOES ABOUT THIS AND SUCH OTHER ISSUES !!! ???? uSHA DI ===== On Wed, Jan 11, 2012 at 9:03 PM, Vijayasankar <[email protected]>wrote: > I thank you all for the lively interactions. Thanks to Gurcharan ji, > Pankaj and Usha di for expert inputs on SEM/TEM. The paper on crystals is > really a good one, thanks Pankaj for sharing. > I thank Aarti ji, Balkar ji, Bhagyashri ji, Nidhan ji, Garg ji, Neha ji, > Betab Singh ji and Prashant ji for their appreciations and interactions. It > was a great experience :) > > > Regards > > Vijayasankar Raman > National Center for Natural Products Research > University of Mississippi > > > On Wed, Jan 11, 2012 at 8:30 AM, Balkar Singh <[email protected]>wrote: > >> Really good discussion i learnt a lot about SEM TEM >> >> >> On Wed, Jan 11, 2012 at 2:06 PM, Gurcharan Singh <[email protected]>wrote: >> >>> Thanks a lot Ushadi >>> >>> >>> -- >>> Dr. Gurcharan Singh >>> Retired Associate Professor >>> SGTB Khalsa College, University of Delhi, Delhi-110007 >>> Res: 932 Anand Kunj, Vikas Puri, New Delhi-110018. >>> Phone: 011-25518297 Mob: 9810359089 >>> http://people.du.ac.in/~singhg45/ >>> >>> On Wed, Jan 11, 2012 at 1:25 PM, ushadi Micromini < >>> [email protected]> wrote: >>> >>>> Gurchranji: >>>> Micromorphology is fine... or Microtopography may be apt... >>>> just because the new SEM machines can look even closer, like at 300,000 >>>> magnification, still does not make it look at thin sections and the inside >>>> cytoplasm etc... Whatever is on the surface, including surface of >>>> whatever is inside cells or sacks etc that can be fractured open is looked >>>> at... >>>> >>>> But there is one caveat... if one purifies actin etc of cytoskeletal >>>> structures of a cell and then plates them, dries them, coats etc processes >>>> them, one can look at its structure, but that still is the surface >>>> morphology... of protein or whatever... >>>> >>>> Japanese market now has an open chamber SEM where wet tissues without >>>> processing or drying can be examined... I personally have not seen it, only >>>> a small brochure... so cant comment on it... >>>> >>>> ==== >>>> usha di >>>> =========== >>>> >>>> >>>> >>>> >>>> >>>> On Wed, Jan 11, 2012 at 9:54 AM, Gurcharan Singh <[email protected]>wrote: >>>> >>>>> Thanks Vijayasankar ji and Pankaj ji for these papers. >>>>> I have just given final approval to 3rd Indian edition "Plant >>>>> Systematics, Theory and Practice" due in January 2012 in which I have >>>>> managed to incorporate three major changes of 18th IBC (changing name from >>>>> ICBN to ICN, allowing English description or diagnosis for Valid >>>>> publication and allowing online publication for Effective publication; in >>>>> addition to single name for each Fungus (no separate form genera) and >>>>> fossils), as also incorporating Takhtajan (2009) and APG III >>>>> classifications (which I could not do in 3rd International Edition "Plant >>>>> Systematics, An Integrated Approach, which had gone out from me in >>>>> September 2009, and published in January 2010). Wish I had this >>>>> information >>>>> (courtesy Ushadi, Pankaj ji and Vijayasankar ji) earlier to incorporate in >>>>> my chapter on Ultrastructure and micromorphology. >>>>> Just seeking a suggestion. For last at least three decades or so >>>>> TEM structures were characterized as ultrastructure and SEM structures as >>>>> Micromorphology in most books on Plant Systematics. Now that SEM can also >>>>> explore anatomical features, what could be an appropriate term to cover >>>>> these studies?. I was thinking about appropriate title for my chapter >>>>> which >>>>> is now "Utrastructure and Micromorphology". >>>>> >>>>> >>>>> -- >>>>> Dr. Gurcharan Singh >>>>> Retired Associate Professor >>>>> SGTB Khalsa College, University of Delhi, Delhi-110007 >>>>> Res: 932 Anand Kunj, Vikas Puri, New Delhi-110018. >>>>> Phone: 011-25518297 Mob: 9810359089 >>>>> http://people.du.ac.in/~singhg45/ >>>>> >>>>> >>>>> >>>>> On Wed, Jan 11, 2012 at 6:16 AM, Dr Pankaj Kumar < >>>>> [email protected]> wrote: >>>>> >>>>>> http://epress.anu.edu.au/wp-content/uploads/2011/05/ch0811.pdf >>>>>> >>>>>> Sir you may check this. >>>>>> Pankaj >>>>>> >>>>>> >>>>>> >>>>>> On Jan 10, 6:18 pm, Prashant Awale <[email protected]> wrote: >>>>>> > Dear Vijayasankar ji, >>>>>> > >>>>>> > I really liked this post. Its really a "divine feeling." Thanks >>>>>> for this >>>>>> > unique post. >>>>>> > Regards >>>>>> > Prashant >>>>>> > On Tue, Jan 10, 2012 at 3:08 PM, Ushadi micromini < >>>>>> [email protected] >>>>>> >>>>>> > >>>>>> > >>>>>> > >>>>>> > >>>>>> > >>>>>> > >>>>>> > >>>>>> > > wrote: >>>>>> > > Gurcharanji: >>>>>> > > I dont mean to correct you... >>>>>> > > but all are SEM..... >>>>>> > >>>>>> > > not a single one of these is a TEM.... >>>>>> > >>>>>> > > Usha di >>>>>> > > ========== >>>>>> > >>>>>> > > On Jan 10, 1:52 pm, Gurcharan Singh <[email protected]> wrote: >>>>>> > > > Thanks Pankaj ji and Ushadi >>>>>> > > > I read second mail of Vijay ji and somehow missed looking at >>>>>> attachments. >>>>>> > > > Thanks for bringing it to my notice. >>>>>> > >>>>>> > > > Should we say >>>>>> > > > First picture is SEM of leaf showing trichomes >>>>>> > > > Second and third picture are TEM of cells showing crystals >>>>>> > > > 4th again seems to be SEM >>>>>> > >>>>>> > > > -- >>>>>> > > > Dr. Gurcharan Singh >>>>>> > > > Retired Associate Professor >>>>>> > > > SGTB Khalsa College, University of Delhi, Delhi-110007 >>>>>> > > > Res: 932 Anand Kunj, Vikas Puri, New Delhi-110018. >>>>>> > > > Phone: 011-25518297 Mob: 9810359089 >>>>>> http://people.du.ac.in/~singhg45/ >>>>>> > >>>>>> > > > On Tue, Jan 10, 2012 at 2:11 PM, Ushadi micromini < >>>>>> > > [email protected] >>>>>> > >>>>>> > > > > wrote: >>>>>> > >>>>>> > > > > Gurcharanji: >>>>>> > > > > if you go to abridged summary that came this morning and go >>>>>> to the >>>>>> > > > > first email... its from Raman... and its title is :" My >>>>>> Flora >>>>>> > > > > Picture of the Year 2011: Vijayasankar Raman " >>>>>> > >>>>>> > > > > there you scroll down ... and just above my first comment ... >>>>>> are the >>>>>> > > > > SEM pics ... i was asking about... >>>>>> > >>>>>> > > > > Is n't it wonderful.... >>>>>> > > > > ??? >>>>>> > >>>>>> > > > > love it >>>>>> > > > > Usha di >>>>>> > > > > ============ >>>>>> > >>>>>> > > > > On Jan 10, 1:37 pm, ushadi Micromini < >>>>>> [email protected]> >>>>>> > > > > wrote: >>>>>> > > > > > Thanks Pankaj... >>>>>> > > > > > crystals I could tell... >>>>>> > >>>>>> > > > > > My q to Vijaya was / is : is it cell wall... that's so >>>>>> rigid and >>>>>> > > forming >>>>>> > > > > > the rhomboid spongy looking mesh... >>>>>> > >>>>>> > > > > > usha di >>>>>> > > > > > ======== >>>>>> > >>>>>> > > > > > On Tue, Jan 10, 2012 at 1:55 PM, Pankaj Kumar < >>>>>> > > [email protected] >>>>>> > > > > >wrote: >>>>>> > >>>>>> > > > > > > Mam, 2nd and third pics show crystals (raphides) inside >>>>>> the cell. >>>>>> > > > > > > These crystals as well as their structure sometimes form >>>>>> a good >>>>>> > > > > > > taxonomic character of groups. >>>>>> > > > > > > Pankaj >>>>>> > >>>>>> > > > > > > On Tue, Jan 10, 2012 at 4:19 PM, Ushadi micromini >>>>>> > > > > > > <[email protected]> wrote: >>>>>> > > > > > > > Dear Vijayashankar: >>>>>> > >>>>>> > > > > > > > A I am so excited ... >>>>>> > >>>>>> > > > > > > > 1: first for you, this will add some new or deeper >>>>>> insights >>>>>> > > to >>>>>> > > > > > > > your ongoing studies.... but of course you knew that...! >>>>>> > > > > > > > 2: I am excited for myself and the whole of >>>>>> eflora >>>>>> > > family... >>>>>> > > > > > > > we will get to see the botanical specimen in further >>>>>> details... >>>>>> > > as >>>>>> > > > > and >>>>>> > > > > > > > when you share your finds... >>>>>> > >>>>>> > > > > > > > ================== >>>>>> > >>>>>> > > > > > > > B: >>>>>> > >>>>>> > > > > > > > Now, you will be Vijyashankar micromini .... >>>>>> > > > > > > > instead of / in addition to me... >>>>>> > >>>>>> > > > > > > > the micromini epithet was attached to my name by a few >>>>>> friends >>>>>> > > and >>>>>> > > > > > > > colleagues a couple or three decades ago because I was >>>>>> fond of >>>>>> > > doing >>>>>> > > > > > > > TEM and SEM of tumors etc....and ribosome...and >>>>>> mitochondria... >>>>>> > >>>>>> > > > > > > > ======== >>>>>> > >>>>>> > > > > > > > C: and this the question section :-) >>>>>> > >>>>>> > > > > > > > the first pic is surface of leaf..... i imagine.... >>>>>> > >>>>>> > > > > > > > but what are 2 and 3rd pictures of ... a fracture of >>>>>> what? >>>>>> > >>>>>> > > > > > > > is it of a leaf and the rhoboid? structures cell >>>>>> walls... with >>>>>> > > > > > > > crystals etc inside? >>>>>> > >>>>>> > > > > > > > ============== >>>>>> > >>>>>> > > > > > > > just cant stop smiling.... what a great new dimension >>>>>> to our >>>>>> > > > > > > > interaction will this make.... >>>>>> > > > > > > > wonderful for all of us... >>>>>> > >>>>>> > > > > > > > and congratulations for learning the techniques... >>>>>> > > > > > > > ushadi >>>>>> > >>>>>> > > > > > > > ========== >>>>>> > > > > > > > ps does this grow in mississipi??? >>>>>> > >>>>>> > > > > > > > ================= >>>>>> > >>>>>> > > > > > > > On Jan 10, 12:44 pm, "J.M. Garg" <[email protected]> >>>>>> wrote: >>>>>> > > > > > > >> Thanks, Vijayasankar ji, >>>>>> > > > > > > >> Wonderly different idea & picture. >>>>>> > >>>>>> > > > > > > >> For others: >>>>>> > > > > > > >> Scanning Electron Microscopy (SEM): >>>>>> > >>>>>> > > >>>>>> http://serc.carleton.edu/research_education/geochemsheets/techniques/ >>>>>> . >>>>>> > > > > .. >>>>>> > >>>>>> > > > > > > >> A link for Scanning electron microscope: >>>>>> > > > > > >http://en.wikipedia.org/wiki/Scanning_electron_microscope >>>>>> > >>>>>> > > > > > > >> On 10 January 2012 05:15, Vijayasankar < >>>>>> > > [email protected]> >>>>>> > > > > > > wrote: >>>>>> > >>>>>> > > > > > > >> > Dear all >>>>>> > >>>>>> > > > > > > >> > Here is yet another b&w picture as 'my flora picture >>>>>> of the >>>>>> > > year >>>>>> > > > > > > 2011'. >>>>>> > > > > > > >> > I am not sure if this is suitable for this title, >>>>>> though. >>>>>> > >>>>>> > > > > > > >> > But this is my first ever SEM picture :) It was my >>>>>> > > > > dream....finally >>>>>> > > > > > > came >>>>>> > > > > > > >> > true! It was a great experience to learn the >>>>>> techniques >>>>>> > > without a >>>>>> > > > > > > tutor >>>>>> > > > > > > >> > (but now I am helping many...). >>>>>> > > > > > > >> > The picture shows glandular and non-glandular >>>>>> trichomes from >>>>>> > > leaf >>>>>> > > > > of >>>>>> > > > > > > >> > Salvia divinorum, a psychoactive plant endemic to >>>>>> Mexico. >>>>>> > > > > > > >> > I recorded 5 types and 33 sub-types of trichomes >>>>>> (some of them >>>>>> > > > > are new >>>>>> > > > > > > >> > reports), from a single leaf, thanks to SEM... >>>>>> > >>>>>> > > > > > > >> > Regards >>>>>> > >>>>>> > > > > > > >> > Vijayasankar Raman >>>>>> > > > > > > >> > National Center for Natural Products Research >>>>>> > > > > > > >> > University of Mississippi >>>>>> > >>>>>> > > > > > > >> -- >>>>>> > > > > > > >> With regards, >>>>>> > > > > > > >> J.M.Garg ([email protected]) >>>>>> > > > >http://en.wikipedia.org/wiki/User:Jmgarg1 >>>>>> > > > > > > >> 'Creating awareness of Indian Flora & Fauna' >>>>>> > > > > > > >> The whole world uses my Image Resource of more than a >>>>>> *thousand >>>>>> > > > > > > species* & >>>>>> > > > > > > >> eight thousand images of Birds, Butterflies, Plants >>>>>> etc. >>>>>> > > (arranged >>>>>> > > > > > > >> alphabetically & place-wise): >>>>>> > > > > > >http://commons.wikimedia.org/wiki/Category:J.M.Garg. You >>>>>> can also >>>>>> > > use >>>>>> > > > > them >>>>>> > > > > > > >> for free as per Creative Commons license attached with >>>>>> each >>>>>> > > image. >>>>>> > > > > > > >> For identification, learning, discussion & >>>>>> documentation of >>>>>> > > Indian >>>>>> > > > > > > Flora, >>>>>> > > > > > > >> please visit/ join our Efloraofindia Google e-group: >>>>>> > > > > > > >>>>>> http://groups.google.co.in/group/indiantreepix(morethan1760members& >>>>>> > > > > > > >> 1,00,000 messages on 21/12/11) or Efloraofindia >>>>>> website: >>>>>> > > > > > > >>>>>> https://sites.google.com/site/efloraofindia/(withaspeciesdatabase >>>>>> > > > > > > >> of more than 6000 species). >>>>>> > > > > > > >> Also author of 'A Photoguide to the Birds of Kolkata & >>>>>> Common >>>>>> > > Birds >>>>>> > > > > of >>>>>> > > > > > > >> India'. >>>>>> > >>>>>> > > > > > > -- >>>>>> > >>>>>> > > >>>>>> ********************************************************************** >>>>>> > > > > > > "Taxonomists getting Extinct and Species Data Deficient >>>>>> !!" >>>>>> > >>>>>> > > > > > > Pankaj Kumar Ph.D. (Orchidaceae) >>>>>> > > > > > > Conservation Officer >>>>>> > >>>>>> > > > > > > Office: >>>>>> > > > > > > Orchid Conservation Section >>>>>> > > > > > > Flora Conservation Department >>>>>> > > > > > > Kadoorie Farm and Botanic Garden (KFBG) Corporation >>>>>> > > > > > > Lam Kam Road, Tai Po, New Territories, Hong Kong. >>>>>> > >>>>>> > > > > > > Residence: >>>>>> > > > > > > 36c, Ng Tung Chai, Lam Tseun >>>>>> > > > > > > Tai Po, New Territories, Hong Kong. >>>>>> > >>>>>> > > > > > > email: [email protected] >>>>>> > > > > > > [email protected] >>>>>> > > > > > > [email protected] >>>>>> > > > > > > Phone:+852 2483 >>>>>> 7128begin_of_the_skype_highlighting +852 2483 7128 >>>>>> (office >>>>>> - 8:30am to 5:30pm) >>>>>> > > > > > > +852 9436 >>>>>> 6251begin_of_the_skype_highlighting +852 9436 6251 >>>>>> (mobile) >>>>>> > >>>>>> > > > > > -- >>>>>> > > > > > Usha di >>>>>> > > > > > =========== >>>>> >>>>> >>>>> >>>>> >>>>> >>>> >>>> >>>> -- >>>> Usha di >>>> =========== >>>> >>>> >>> >>> >>> >> >> >> -- >> Regards >> >> Dr Balkar Singh >> Head, Deptt. of Botany and Biotechnology >> Arya P G College, Panipat >> Haryana-132103 >> 09416262964 >> > > -- Usha di ===========
