I thank you all for the lively interactions. Thanks to Gurcharan ji, Pankaj and Usha di for expert inputs on SEM/TEM. The paper on crystals is really a good one, thanks Pankaj for sharing. I thank Aarti ji, Balkar ji, Bhagyashri ji, Nidhan ji, Garg ji, Neha ji, Betab Singh ji and Prashant ji for their appreciations and interactions. It was a great experience :)
Regards Vijayasankar Raman National Center for Natural Products Research University of Mississippi On Wed, Jan 11, 2012 at 8:30 AM, Balkar Singh <[email protected]> wrote: > Really good discussion i learnt a lot about SEM TEM > > > On Wed, Jan 11, 2012 at 2:06 PM, Gurcharan Singh <[email protected]>wrote: > >> Thanks a lot Ushadi >> >> >> -- >> Dr. Gurcharan Singh >> Retired Associate Professor >> SGTB Khalsa College, University of Delhi, Delhi-110007 >> Res: 932 Anand Kunj, Vikas Puri, New Delhi-110018. >> Phone: 011-25518297 Mob: 9810359089 >> http://people.du.ac.in/~singhg45/ >> >> On Wed, Jan 11, 2012 at 1:25 PM, ushadi Micromini < >> [email protected]> wrote: >> >>> Gurchranji: >>> Micromorphology is fine... or Microtopography may be apt... >>> just because the new SEM machines can look even closer, like at 300,000 >>> magnification, still does not make it look at thin sections and the inside >>> cytoplasm etc... Whatever is on the surface, including surface of >>> whatever is inside cells or sacks etc that can be fractured open is looked >>> at... >>> >>> But there is one caveat... if one purifies actin etc of cytoskeletal >>> structures of a cell and then plates them, dries them, coats etc processes >>> them, one can look at its structure, but that still is the surface >>> morphology... of protein or whatever... >>> >>> Japanese market now has an open chamber SEM where wet tissues without >>> processing or drying can be examined... I personally have not seen it, only >>> a small brochure... so cant comment on it... >>> >>> ==== >>> usha di >>> =========== >>> >>> >>> >>> >>> >>> On Wed, Jan 11, 2012 at 9:54 AM, Gurcharan Singh <[email protected]>wrote: >>> >>>> Thanks Vijayasankar ji and Pankaj ji for these papers. >>>> I have just given final approval to 3rd Indian edition "Plant >>>> Systematics, Theory and Practice" due in January 2012 in which I have >>>> managed to incorporate three major changes of 18th IBC (changing name from >>>> ICBN to ICN, allowing English description or diagnosis for Valid >>>> publication and allowing online publication for Effective publication; in >>>> addition to single name for each Fungus (no separate form genera) and >>>> fossils), as also incorporating Takhtajan (2009) and APG III >>>> classifications (which I could not do in 3rd International Edition "Plant >>>> Systematics, An Integrated Approach, which had gone out from me in >>>> September 2009, and published in January 2010). Wish I had this information >>>> (courtesy Ushadi, Pankaj ji and Vijayasankar ji) earlier to incorporate in >>>> my chapter on Ultrastructure and micromorphology. >>>> Just seeking a suggestion. For last at least three decades or so TEM >>>> structures were characterized as ultrastructure and SEM structures as >>>> Micromorphology in most books on Plant Systematics. Now that SEM can also >>>> explore anatomical features, what could be an appropriate term to cover >>>> these studies?. I was thinking about appropriate title for my chapter which >>>> is now "Utrastructure and Micromorphology". >>>> >>>> >>>> -- >>>> Dr. Gurcharan Singh >>>> Retired Associate Professor >>>> SGTB Khalsa College, University of Delhi, Delhi-110007 >>>> Res: 932 Anand Kunj, Vikas Puri, New Delhi-110018. >>>> Phone: 011-25518297 Mob: 9810359089 >>>> http://people.du.ac.in/~singhg45/ >>>> >>>> >>>> >>>> On Wed, Jan 11, 2012 at 6:16 AM, Dr Pankaj Kumar < >>>> [email protected]> wrote: >>>> >>>>> http://epress.anu.edu.au/wp-content/uploads/2011/05/ch0811.pdf >>>>> >>>>> Sir you may check this. >>>>> Pankaj >>>>> >>>>> >>>>> >>>>> On Jan 10, 6:18 pm, Prashant Awale <[email protected]> wrote: >>>>> > Dear Vijayasankar ji, >>>>> > >>>>> > I really liked this post. Its really a "divine feeling." Thanks for >>>>> this >>>>> > unique post. >>>>> > Regards >>>>> > Prashant >>>>> > On Tue, Jan 10, 2012 at 3:08 PM, Ushadi micromini < >>>>> [email protected] >>>>> >>>>> > >>>>> > >>>>> > >>>>> > >>>>> > >>>>> > >>>>> > >>>>> > > wrote: >>>>> > > Gurcharanji: >>>>> > > I dont mean to correct you... >>>>> > > but all are SEM..... >>>>> > >>>>> > > not a single one of these is a TEM.... >>>>> > >>>>> > > Usha di >>>>> > > ========== >>>>> > >>>>> > > On Jan 10, 1:52 pm, Gurcharan Singh <[email protected]> wrote: >>>>> > > > Thanks Pankaj ji and Ushadi >>>>> > > > I read second mail of Vijay ji and somehow missed looking at >>>>> attachments. >>>>> > > > Thanks for bringing it to my notice. >>>>> > >>>>> > > > Should we say >>>>> > > > First picture is SEM of leaf showing trichomes >>>>> > > > Second and third picture are TEM of cells showing crystals >>>>> > > > 4th again seems to be SEM >>>>> > >>>>> > > > -- >>>>> > > > Dr. Gurcharan Singh >>>>> > > > Retired Associate Professor >>>>> > > > SGTB Khalsa College, University of Delhi, Delhi-110007 >>>>> > > > Res: 932 Anand Kunj, Vikas Puri, New Delhi-110018. >>>>> > > > Phone: 011-25518297 Mob: 9810359089 >>>>> http://people.du.ac.in/~singhg45/ >>>>> > >>>>> > > > On Tue, Jan 10, 2012 at 2:11 PM, Ushadi micromini < >>>>> > > [email protected] >>>>> > >>>>> > > > > wrote: >>>>> > >>>>> > > > > Gurcharanji: >>>>> > > > > if you go to abridged summary that came this morning and go to >>>>> the >>>>> > > > > first email... its from Raman... and its title is :" My >>>>> Flora >>>>> > > > > Picture of the Year 2011: Vijayasankar Raman " >>>>> > >>>>> > > > > there you scroll down ... and just above my first comment ... >>>>> are the >>>>> > > > > SEM pics ... i was asking about... >>>>> > >>>>> > > > > Is n't it wonderful.... >>>>> > > > > ??? >>>>> > >>>>> > > > > love it >>>>> > > > > Usha di >>>>> > > > > ============ >>>>> > >>>>> > > > > On Jan 10, 1:37 pm, ushadi Micromini < >>>>> [email protected]> >>>>> > > > > wrote: >>>>> > > > > > Thanks Pankaj... >>>>> > > > > > crystals I could tell... >>>>> > >>>>> > > > > > My q to Vijaya was / is : is it cell wall... that's so >>>>> rigid and >>>>> > > forming >>>>> > > > > > the rhomboid spongy looking mesh... >>>>> > >>>>> > > > > > usha di >>>>> > > > > > ======== >>>>> > >>>>> > > > > > On Tue, Jan 10, 2012 at 1:55 PM, Pankaj Kumar < >>>>> > > [email protected] >>>>> > > > > >wrote: >>>>> > >>>>> > > > > > > Mam, 2nd and third pics show crystals (raphides) inside >>>>> the cell. >>>>> > > > > > > These crystals as well as their structure sometimes form a >>>>> good >>>>> > > > > > > taxonomic character of groups. >>>>> > > > > > > Pankaj >>>>> > >>>>> > > > > > > On Tue, Jan 10, 2012 at 4:19 PM, Ushadi micromini >>>>> > > > > > > <[email protected]> wrote: >>>>> > > > > > > > Dear Vijayashankar: >>>>> > >>>>> > > > > > > > A I am so excited ... >>>>> > >>>>> > > > > > > > 1: first for you, this will add some new or deeper >>>>> insights >>>>> > > to >>>>> > > > > > > > your ongoing studies.... but of course you knew that...! >>>>> > > > > > > > 2: I am excited for myself and the whole of >>>>> eflora >>>>> > > family... >>>>> > > > > > > > we will get to see the botanical specimen in further >>>>> details... >>>>> > > as >>>>> > > > > and >>>>> > > > > > > > when you share your finds... >>>>> > >>>>> > > > > > > > ================== >>>>> > >>>>> > > > > > > > B: >>>>> > >>>>> > > > > > > > Now, you will be Vijyashankar micromini .... >>>>> > > > > > > > instead of / in addition to me... >>>>> > >>>>> > > > > > > > the micromini epithet was attached to my name by a few >>>>> friends >>>>> > > and >>>>> > > > > > > > colleagues a couple or three decades ago because I was >>>>> fond of >>>>> > > doing >>>>> > > > > > > > TEM and SEM of tumors etc....and ribosome...and >>>>> mitochondria... >>>>> > >>>>> > > > > > > > ======== >>>>> > >>>>> > > > > > > > C: and this the question section :-) >>>>> > >>>>> > > > > > > > the first pic is surface of leaf..... i imagine.... >>>>> > >>>>> > > > > > > > but what are 2 and 3rd pictures of ... a fracture of >>>>> what? >>>>> > >>>>> > > > > > > > is it of a leaf and the rhoboid? structures cell >>>>> walls... with >>>>> > > > > > > > crystals etc inside? >>>>> > >>>>> > > > > > > > ============== >>>>> > >>>>> > > > > > > > just cant stop smiling.... what a great new dimension >>>>> to our >>>>> > > > > > > > interaction will this make.... >>>>> > > > > > > > wonderful for all of us... >>>>> > >>>>> > > > > > > > and congratulations for learning the techniques... >>>>> > > > > > > > ushadi >>>>> > >>>>> > > > > > > > ========== >>>>> > > > > > > > ps does this grow in mississipi??? >>>>> > >>>>> > > > > > > > ================= >>>>> > >>>>> > > > > > > > On Jan 10, 12:44 pm, "J.M. Garg" <[email protected]> >>>>> wrote: >>>>> > > > > > > >> Thanks, Vijayasankar ji, >>>>> > > > > > > >> Wonderly different idea & picture. >>>>> > >>>>> > > > > > > >> For others: >>>>> > > > > > > >> Scanning Electron Microscopy (SEM): >>>>> > >>>>> > > >>>>> http://serc.carleton.edu/research_education/geochemsheets/techniques/. >>>>> > > > > .. >>>>> > >>>>> > > > > > > >> A link for Scanning electron microscope: >>>>> > > > > > >http://en.wikipedia.org/wiki/Scanning_electron_microscope >>>>> > >>>>> > > > > > > >> On 10 January 2012 05:15, Vijayasankar < >>>>> > > [email protected]> >>>>> > > > > > > wrote: >>>>> > >>>>> > > > > > > >> > Dear all >>>>> > >>>>> > > > > > > >> > Here is yet another b&w picture as 'my flora picture >>>>> of the >>>>> > > year >>>>> > > > > > > 2011'. >>>>> > > > > > > >> > I am not sure if this is suitable for this title, >>>>> though. >>>>> > >>>>> > > > > > > >> > But this is my first ever SEM picture :) It was my >>>>> > > > > dream....finally >>>>> > > > > > > came >>>>> > > > > > > >> > true! It was a great experience to learn the >>>>> techniques >>>>> > > without a >>>>> > > > > > > tutor >>>>> > > > > > > >> > (but now I am helping many...). >>>>> > > > > > > >> > The picture shows glandular and non-glandular >>>>> trichomes from >>>>> > > leaf >>>>> > > > > of >>>>> > > > > > > >> > Salvia divinorum, a psychoactive plant endemic to >>>>> Mexico. >>>>> > > > > > > >> > I recorded 5 types and 33 sub-types of trichomes >>>>> (some of them >>>>> > > > > are new >>>>> > > > > > > >> > reports), from a single leaf, thanks to SEM... >>>>> > >>>>> > > > > > > >> > Regards >>>>> > >>>>> > > > > > > >> > Vijayasankar Raman >>>>> > > > > > > >> > National Center for Natural Products Research >>>>> > > > > > > >> > University of Mississippi >>>>> > >>>>> > > > > > > >> -- >>>>> > > > > > > >> With regards, >>>>> > > > > > > >> J.M.Garg ([email protected]) >>>>> > > > >http://en.wikipedia.org/wiki/User:Jmgarg1 >>>>> > > > > > > >> 'Creating awareness of Indian Flora & Fauna' >>>>> > > > > > > >> The whole world uses my Image Resource of more than a >>>>> *thousand >>>>> > > > > > > species* & >>>>> > > > > > > >> eight thousand images of Birds, Butterflies, Plants etc. >>>>> > > (arranged >>>>> > > > > > > >> alphabetically & place-wise): >>>>> > > > > > >http://commons.wikimedia.org/wiki/Category:J.M.Garg. You >>>>> can also >>>>> > > use >>>>> > > > > them >>>>> > > > > > > >> for free as per Creative Commons license attached with >>>>> each >>>>> > > image. >>>>> > > > > > > >> For identification, learning, discussion & >>>>> documentation of >>>>> > > Indian >>>>> > > > > > > Flora, >>>>> > > > > > > >> please visit/ join our Efloraofindia Google e-group: >>>>> > > > > > > >>>>> http://groups.google.co.in/group/indiantreepix(morethan1760members& >>>>> > > > > > > >> 1,00,000 messages on 21/12/11) or Efloraofindia website: >>>>> > > > > > > >>>>> https://sites.google.com/site/efloraofindia/(withaspeciesdatabase >>>>> > > > > > > >> of more than 6000 species). >>>>> > > > > > > >> Also author of 'A Photoguide to the Birds of Kolkata & >>>>> Common >>>>> > > Birds >>>>> > > > > of >>>>> > > > > > > >> India'. >>>>> > >>>>> > > > > > > -- >>>>> > >>>>> > > >>>>> ********************************************************************** >>>>> > > > > > > "Taxonomists getting Extinct and Species Data Deficient !!" >>>>> > >>>>> > > > > > > Pankaj Kumar Ph.D. (Orchidaceae) >>>>> > > > > > > Conservation Officer >>>>> > >>>>> > > > > > > Office: >>>>> > > > > > > Orchid Conservation Section >>>>> > > > > > > Flora Conservation Department >>>>> > > > > > > Kadoorie Farm and Botanic Garden (KFBG) Corporation >>>>> > > > > > > Lam Kam Road, Tai Po, New Territories, Hong Kong. >>>>> > >>>>> > > > > > > Residence: >>>>> > > > > > > 36c, Ng Tung Chai, Lam Tseun >>>>> > > > > > > Tai Po, New Territories, Hong Kong. >>>>> > >>>>> > > > > > > email: [email protected] >>>>> > > > > > > [email protected] >>>>> > > > > > > [email protected] >>>>> > > > > > > Phone:+852 2483 >>>>> 7128begin_of_the_skype_highlighting +852 2483 7128 (office >>>>> - 8:30am to 5:30pm) >>>>> > > > > > > +852 9436 >>>>> 6251begin_of_the_skype_highlighting +852 9436 6251 >>>>> (mobile) >>>>> > >>>>> > > > > > -- >>>>> > > > > > Usha di >>>>> > > > > > =========== >>>> >>>> >>>> >>>> >>>> >>> >>> >>> -- >>> Usha di >>> =========== >>> >>> >> >> >> > > > -- > Regards > > Dr Balkar Singh > Head, Deptt. of Botany and Biotechnology > Arya P G College, Panipat > Haryana-132103 > 09416262964 >
