Hi there, I would not recommend concentrating samples by lyophilization or speed-vac. Both concentrate salts, buffers and detergents. I tried to concentrate conditioned medium for doing SDS-PAGE. The samples were concentrated about 10 times. However, the ppt was not fully re-solublized. I don't know what happened to the protein, the result was I saw nothing on the membrane. I believe the solubility of proteins and salts concentration are the problems.
Best, Stan ________________________________ From: lautys <lau...@gmail.com> To: DK <d...@no.email.thankstospam.net> Cc: meth...@magpie.bio.indiana.edu Sent: Fri, March 12, 2010 9:33:15 AM Subject: Re: Protein precipitation - acetone? dear all, how about lyophilize low protein concentration sample and resuspend in lower volume of buffer/water? is that recommended for protein concentration? regards, Lau _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods Send instant messages to your online friends http://uk.messenger.yahoo.com _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
