Hello Stan,
I am sorry about your problems using speed-vac. I am used to concentratre samples containing 150mM NaCl, 0.1% NP-40 even 10X and if I boiled them longer (10 minutes) and run the gel slowly at the beginning it works fine. I don't know, but maybe it's worth to give it a try! Regards, Eva > Date: Sun, 14 Mar 2010 19:35:25 -0700 > From: stanleycheun...@yahoo.com > To: meth...@magpie.bio.indiana.edu > CC: > Subject: Re: Protein precipitation - acetone? > > Hi there, > > I would not recommend concentrating samples by lyophilization or speed-vac. > Both concentrate salts, buffers and detergents. I tried to concentrate > conditioned medium for doing SDS-PAGE. The samples were concentrated about 10 > times. However, the ppt was not fully re-solublized. I don't know what > happened to the protein, the result was I saw nothing on the membrane. I > believe the solubility of proteins and salts concentration are the problems. > > Best, > Stan > > > > ________________________________ > From: lautys <lau...@gmail.com> > To: DK <d...@no.email.thankstospam.net> > Cc: meth...@magpie.bio.indiana.edu > Sent: Fri, March 12, 2010 9:33:15 AM > Subject: Re: Protein precipitation - acetone? > > dear all, > > how about lyophilize low protein concentration sample and resuspend in lower > volume of buffer/water? is that recommended for protein concentration? > > regards, > Lau > _______________________________________________ > Methods mailing list > Methods@net.bio.net > http://www.bio.net/biomail/listinfo/methods > > > Send instant messages to your online friends http://uk.messenger.yahoo.com > _______________________________________________ > Methods mailing list > Methods@net.bio.net > http://www.bio.net/biomail/listinfo/methods _________________________________________________________________ ¿Te gustaría tener Hotmail en tu móvil Movistar? ¡Es gratis! http://serviciosmoviles.es.msn.com/hotmail/movistar-particulares.aspx_______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
