Come to think of it.. now and then I seem to have seen a strange plasmid in some of my transformations. I wonder have I been seeing the plasmid for the ligase I have been using from Roche? It was on Ampicillin agar dishes. Suddenly I regret discarding those cultures! Thanks for this tip DK, I'm inspired to start experimenting with it now.
On 27 July 2010 21:28, Cathal Garvey <cathalgar...@gmail.com> wrote: > That is ingenious. I was fretting that the gene or peptide sequence for > SpeI is apparently not known or available online, and access to that > particular bacterial strain would be very costly. If I can transform from > sacI enzyme and get the plasmid for sequencing, it will be a joyous > occasion! > > --- > Twitter: @onetruecathal > Sent from my beloved Android phone. > > On 27 Jul 2010 17:46, "DK" <d...@no.email.thankstospam.net> wrote: > > In article <mailman.912.1280230802.25217.meth...@net.bio.net>, jh < > bio...@gmail.com> wrote: > >Dear al... > Have really highly competent cells and possibly try several Taq > suppliers: "transform" cells with the protein prep. Virtually every > recombinant protein prep is contaminated at a very low level with > the recombinant plasmid. Since it is not known what resistance > marker was used, plate on amp, kan and cam plates. > > I have used this trick to get several genes. > > DK > > > > > _______________________________________________ > Methods mailing list > Methods@net.bio.net > http://... > > -- letters.cunningprojects.com twitter.com/onetruecathal twitter.com/labsfromfabs _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
