When you have got the plasmid, you need to sequence it, of course, to verify it's a Taq and to determine suitable primer sequences. For a start, frequently occurring elements like T4, T7, Sp6, His tag etc. should be useful, you might find appropriate primers in your lab's fridge. You might want to search the internet for some commonly used plasmids like pCDNA3 or dig in the tech section of your NEB catalogue to obtain lots of structural information on how plasmids look like.
Happy hacking! Wo _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
