Hi DK, is your trick only applicable to Taq? Actually, it should work with every recombinant protein, will it?
Wo > >I write to search for a plasmid containing Taq DNA polymerase gene. > >Anyone who have this plasmid would be kind to help me? > >Thanks in advance. > > Have really highly competent cells and possibly try several Taq > suppliers: "transform" cells with the protein prep. Virtually every > recombinant protein prep is contaminated at a very low level with > the recombinant plasmid. Since it is not known what resistance > marker was used, plate on amp, kan and cam plates. > > I have used this trick to get several genes. > > DK _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
