I imagine restriction enzymes are out, unless the plasmid in question contains the methylase in addition to the restriction enzyme. Even then, the restriction enzyme may dice everything up before the methylase can prevent it. So success is more likely with polymerases, ligases and phosphatases than the average restriction enzyme.
Most plasmids are likely to work in E.coli, considering how prevelant it is in the patents and datasheets, so Top10s would probably work a treat. The question then arises as to whether the proteins are His tagged or otherwise easily purified. On 27 July 2010 21:59, WS <novalidaddr...@nurfuerspam.de> wrote: > Hi DK, > > is your trick only applicable to Taq? Actually, it should work with > every recombinant protein, will it? > > Wo > > > >I write to search for a plasmid containing Taq DNA polymerase gene. > > >Anyone who have this plasmid would be kind to help me? > > >Thanks in advance. > > > > Have really highly competent cells and possibly try several Taq > > suppliers: "transform" cells with the protein prep. Virtually every > > recombinant protein prep is contaminated at a very low level with > > the recombinant plasmid. Since it is not known what resistance > > marker was used, plate on amp, kan and cam plates. > > > > I have used this trick to get several genes. > > > > DK > > _______________________________________________ > Methods mailing list > Methods@net.bio.net > http://www.bio.net/biomail/listinfo/methods > -- letters.cunningprojects.com twitter.com/onetruecathal twitter.com/labsfromfabs _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
