Dk, Finally, something we agree on :-)) Jay
-----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of DK Sent: Thursday, January 05, 2012 11:25 AM To: [email protected] Subject: Re: 10X PBS In article <[email protected]>, Joshua Silverstein <[email protected]> wrote: >You don't need physical chemistry, just general. > >If you take 10X PBS and dilute it with deionized water, it will certainly >change the pH. For a 10-fold dilution, it should change the pH by >approximately 1 (log scale). False. We are talking about buffers here... DK > > >On Wed, Jan 4, 2012 at 3:38 PM, DK <[email protected]> wrote: > >> In article <[email protected]>, "Jayakumar, >> R" <[email protected]> wrote: >> >Ideally the pH should not change much between 10X and 1X if deionised >> water was >> > used for dilution. Presnce of NaCl has nothing to do with pH changes >> since it >> > contributes neither hydroxyl nor hydrogen ions >> >> You need to refresh your physical chemistry. >> (Hint: cations compete with protons for binding to anions) >> >> DK >> >> _______________________________________________ >> Methods mailing list >> [email protected] >> http://www.bio.net/biomail/listinfo/methods >> _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
