Very true, but the change in pH is really not that much within the pKa range and is deionised water has a lower impact on their activities than tap water or distilled water has. I have not tested this and hence I hope I am right :-) Please correct me if I am not. Jay
-----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Dr Engelbert Buxbaum Sent: Sunday, January 08, 2012 7:04 PM To: [email protected] Subject: RE: 10X PBS In article <[email protected]>, [email protected] says... > > I feel that there is no necessity to teach about pH to this group of > scientists. But to know why deionised water cannot change the pH of > buffers significantly Actually, it does, because the pH is determined not by the concentrations of corresponding base and acid, but by their activities. And these change upon dilution, especially when you start with highly concentrated solutions, where non-ideality is more significant. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
