You don't need physical chemistry, just general. If you take 10X PBS and dilute it with deionized water, it will certainly change the pH. For a 10-fold dilution, it should change the pH by approximately 1 (log scale).
On Wed, Jan 4, 2012 at 3:38 PM, DK <[email protected]> wrote: > In article <[email protected]>, "Jayakumar, > R" <[email protected]> wrote: > >Ideally the pH should not change much between 10X and 1X if deionised > water was > > used for dilution. Presnce of NaCl has nothing to do with pH changes > since it > > contributes neither hydroxyl nor hydrogen ions > > You need to refresh your physical chemistry. > (Hint: cations compete with protons for binding to anions) > > DK > > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
