Hi, I'm using this recipe for PBS, suitable for many occasions. Actually, it is a rinsing buffer for cells, prior to trypsinization (D- PBS from PAA), hence no divalent cations like Calcium and Magnesium.
Depending on your application (when you don't apply it on living beings e.g.) , you may replace the potassium salts with sodium salts for simplicity The mean thing is that one needs to be careful about the actual molecular weights of the phosphates as they are available with varying crystal water content, so you might double check the formula and the molecular weight on the actual box. 1xPBS: KCl M= 74,6 g/mol 0.2g/l → 2,68 mM NaCl M= 58.4 g/mol 8g/l → 137 mM KH2PO4 M= 136.1g/mol 0.2g/l → 1.47 mM Na2HPO4 M= 142g/mol 1.16g/l → 8.17 mM Formulation, 10x Concentrate (g/l) Inorganic Salts KCl 2.0 KH2PO4 2.0 NaCl 80.0 Na2HPO4 anhydrous 11.58 HTH Wo _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
