What Bulent said - at the same magnification DOF is essentially the same. I read that in a book yeas ago and at the time it seemed counter intuitive, but I did the test you describe and found that the there was no real difference in DOF.

On 5/30/2015 2:05 PM, Larry Colen wrote:
A question for the macro mavens, Is there an noticeable difference in depth of field of the subject, when using different focal length lenses?

For example, if I were to come in to the distance where I'm at 1:1 magnification with my 35, 50 and 90mm macros, and focus on the 10cm mark on a ruler, would one of the lenses show more depth of field than the others?

The 90mm lens will be furthest away and the 35mm lens would be nearly touching at 1:1, and there would be big differences in how wide an angle of background that you see, but is there much difference in what is in focus?



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