Geiger has an interface to pathd8; it has the beginnings of interfaces to
other programs, too, but I don't think they're finished yet.

Best,
Brian

_______________________________________
Brian O'Meara
Associate Professor
Dept. of Ecology & Evolutionary Biology
U. of Tennessee, Knoxville
http://www.brianomeara.info

Postdoc collaborators wanted: http://nimbios.org/postdocs/
Calendar: http://www.brianomeara.info/calendars/omeara

On Mon, Sep 21, 2015 at 11:30 AM, Franz Krah <f.k...@mailbox.org> wrote:

> Hi Roger,
> Fast Solution: use pathd8 or r8s or treePL. Not in R... pathd8 should be
> the easiest one since you just need a ultrametric tree... 1 sec in parthd8
>
> Cheers, Franz
>
>
>
>
> > On 21.09.2015, at 16:21, Fabian Roger <fabian.ro...@marine.gu.se> wrote:
> >
> > hej everyone
> >
> > I have a large phylogeny based on 16S RNA OTU data (> 7000 OTUs).  In
> order to calculate some metrics of phylogenetic diversity I need to make
> the tree ultrametric. I thought about using the chronos function from the
> ape package in R.
> >
> > However I run into two problems: the main problem is that for the full
> tree the function fails with the error "Error in nlminb(start.para, f, g,
> control = opt.ctrl, lower = LOW, upper = UP) : NA/NaN gradient evaluation”
> >
> > I have seen several question regarding this error on the mailing list,
> but I haven’t seen a solution. Could anybody help with that? Not that if I
> run small subsets of the full tree, it works without problems!
> >
> > Also, I am concerned about runtime issues. A quick simulation suggests
> that runtime roughly scales linearly with the number of OTUs and that 10
> 000 OTUs should run in below 1 h (although I only simulated trees up to 140
> OTUs…) if that’s true it wouldn’t be all that bad, but does anybody know or
> has experience on if thats actually true?
> >
> > thanks a lot for your help!
> >
> > best
> >
> > Fabian
> > _______________________________________________
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