[ccp4bb] looking for proteins with no homologues in pdb

[Scott Horowitz]

For testing purposes, we want to solve structures of proteins that are not in 
the PDB and have no significant sequence homologues in the PDB (i.e. a blast of 
the pdb will get no significant hits). Does anyone happen know a good way to 
find such proteins efficiently? Having an interesting function isn't needed.

Thanks,
Scott

Scott Horowitz, Ph.D.
Assistant Professor
Department of Chemistry & Biochemistry
Knoebel Institute for Healthy Aging
University of Denver

ECS Building
2155 E. Wesley Ave
Denver, CO 80208
Phone: 303-871-4326
Fax: 303-871-7915
Zoom Room: https://udenver.zoom.us/my/scotthorowitz
Email: scott.horow...@du.edu
Office: Room 561   Lab: Room 505




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Re: [ccp4bb] How can I refine the occupancy with Refmac?

[James Holton]

Certainly Elenaor.  Here are a few:

# typical lysozyme
% refmac_occupancy_setup.com 193l.pdb
occupancy refine
occupancy group id 1 chain A residue 1 alt A
occupancy group id 2 chain A residue 1 alt B
occupancy group id 3 chain A residue 59 alt A
occupancy group id 4 chain A residue 59 alt B
occupancy group id 5 chain A residue 86 alt A
occupancy group id 6 chain A residue 86 alt B
occupancy group id 7 chain A residue 109 alt A
occupancy group id 8 chain A residue 109 alt B
occupancy group alts complete  1 2
occupancy group alts complete  3 4
occupancy group alts complete  5 6
occupancy group alts complete  7 8

#
# another small RT structure

refmac_occupancy_setup.com 1aho.pdb
occupancy refine
occupancy group id 1 chain A residue 9 alt A
occupancy group id 2 chain A residue 9 alt B
occupancy group id 3 chain A residue 12 alt A
occupancy group id 4 chain A residue 12 alt B
occupancy group id 5 chain A residue 24 alt A
occupancy group id 6 chain A residue 24 alt B
occupancy group id 7 chain A residue 63 alt A
occupancy group id 8 chain A residue 63 alt B
occupancy group alts complete  1 2
occupancy group alts complete  3 4
occupancy group alts complete  5 6
occupancy group alts complete  7 8

#
# just the sodium in lysozyme
% egrep "NA|LYS" 2hu3.pdb >! temp.pdb
% refmac_occupancy_setup.com temp.pdb allhet
occupancy refine
occupancy group id 1 residue 1 chain A alt A
occupancy group id 2 residue 1 chain A alt B
occupancy group id 3 residue 33 chain A alt A
occupancy group id 4 residue 33 chain A alt B
occupancy group id 5 residue 97 chain A alt A
occupancy group id 6 residue 97 chain A alt B
occupancy group id 7 residue 9001 chain A
occupancy group id 8 residue 9002 chain A
occupancy group id 9 residue 9003 chain A
occupancy group id 10 residue 9004 chain A
occupancy group id 11 residue 9005 chain A
occupancy group alts complete  5 6
occupancy group alts complete  1 2
occupancy group alts complete  3 4
occupancy group alts incomplete 7
occupancy group alts incomplete 8
occupancy group alts incomplete 9
occupancy group alts incomplete 10
occupancy group alts incomplete 11

#--
Where, for this last one I demonstrate how you can select for various 
things by making a scratch PDB file that contains only the atoms you 
want to occupancy-refine.


Hope this helps!

Cheers,

-James Holton
MAD Scientist


On 6/7/2021 11:16 AM, Eleanor Dodson wrote:
James - could you send me a few examples to add to the documentation? 
Lockdown means I cant easily access my own examples - all trapped on 
the lab desktop..

Eleanor

On Mon, 7 Jun 2021 at 17:18, James Holton > wrote:


I wrote a script for auto-generating occupancy refinement
relationships
for refmac. It is perhaps not as sophisticated as what phenix does
internally, but it gets common things right, like if you have a
2-headed
side chains or partial-occupancy metals.

https://bl831.als.lbl.gov/~jamesh/scripts/refmac_occupancy_setup.com


It is a csh/awk shell script. You run it with the name of your
input pdb
file on the command line and it dumps the refmac keywords to a file
called refmac_opts_occ.txt and to the terminal.  If you want every
"hetatm" atom to be refined put "allhet" as a second command-line
argument.

You can then perhaps copy-and-paste this to the relevant GUI window.

-James Holton
MAD Scientist

On 6/7/2021 3:35 AM, Marina Gárdonyi wrote:
> Hi,
>
> I didn't know that I can also enter keywords without a file!
That is a
> good note, thank you!!
>
> Best regards,
> Marina
>
> Zitat von Jon Cooper mailto:jon.b.coo...@protonmail.com>>:
>
>> Hello, the keywords can be entered in refmac gui (in one of the
>> dropdown things), so you don't need a file, as such, but it's
useful
>> to keep a record.
>>
>> Sent from ProtonMail mobile
>>
>>  Original Message 
>> On 5 Jun 2021, 16:39, Marina Gárdonyi wrote:
>>
>>> Hello everyone,
>>>
>>> I am trying to refine structures with Refmac.
>>>
>>> The problem is that nobody from my working group is familiar
with this
>>> programm. They are using Phenix exclusively.
>>>
>>> That's why I need your help. I would like to refine the
occupancy. I
>>> know that I need a keyword file for this, but I have no idea
how to
>>> create such a keyword file.
>>>
>>> Can someone maybe send me a sample file? I think that would
help me. I
>>> have found essential keywords, but I don't know how to build
up such a
>>> file.
>>>
>>> Thank you very much in advance!
>>>
>>> Best regards,
>>> Marina
>>>
>>> --
>>> Marina Gárdonyi
>>>
>>> PhD Student, Research Group Professor Dr. Klebe
>>>
>>> Department of 

Re: [ccp4bb] How can I refine the occupancy with Refmac?

[Eleanor Dodson]

James - could you send me a few examples to add to the documentation?
Lockdown means I cant easily access my own examples - all trapped on the
lab desktop..
Eleanor

On Mon, 7 Jun 2021 at 17:18, James Holton  wrote:

> I wrote a script for auto-generating occupancy refinement relationships
> for refmac. It is perhaps not as sophisticated as what phenix does
> internally, but it gets common things right, like if you have a 2-headed
> side chains or partial-occupancy metals.
>
> https://bl831.als.lbl.gov/~jamesh/scripts/refmac_occupancy_setup.com
>
> It is a csh/awk shell script. You run it with the name of your input pdb
> file on the command line and it dumps the refmac keywords to a file
> called refmac_opts_occ.txt and to the terminal.  If you want every
> "hetatm" atom to be refined put "allhet" as a second command-line argument.
>
> You can then perhaps copy-and-paste this to the relevant GUI window.
>
> -James Holton
> MAD Scientist
>
> On 6/7/2021 3:35 AM, Marina Gárdonyi wrote:
> > Hi,
> >
> > I didn't know that I can also enter keywords without a file! That is a
> > good note, thank you!!
> >
> > Best regards,
> > Marina
> >
> > Zitat von Jon Cooper :
> >
> >> Hello, the keywords can be entered in refmac gui (in one of the
> >> dropdown things), so you don't need a file, as such, but it's useful
> >> to keep a record.
> >>
> >> Sent from ProtonMail mobile
> >>
> >>  Original Message 
> >> On 5 Jun 2021, 16:39, Marina Gárdonyi wrote:
> >>
> >>> Hello everyone,
> >>>
> >>> I am trying to refine structures with Refmac.
> >>>
> >>> The problem is that nobody from my working group is familiar with this
> >>> programm. They are using Phenix exclusively.
> >>>
> >>> That's why I need your help. I would like to refine the occupancy. I
> >>> know that I need a keyword file for this, but I have no idea how to
> >>> create such a keyword file.
> >>>
> >>> Can someone maybe send me a sample file? I think that would help me. I
> >>> have found essential keywords, but I don't know how to build up such a
> >>> file.
> >>>
> >>> Thank you very much in advance!
> >>>
> >>> Best regards,
> >>> Marina
> >>>
> >>> --
> >>> Marina Gárdonyi
> >>>
> >>> PhD Student, Research Group Professor Dr. Klebe
> >>>
> >>> Department of Pharmaceutical Chemistry
> >>>
> >>> Philipps-University Marburg
> >>>
> >>> Marbacher Weg 6, 35032 Marburg, Germany
> >>>
> >>> Phone: +49 6421 28 21392
> >>>
> >>> E-Mail: marina@pharmazie.uni-marburg.de
> >>>
> >>> http://www.agklebe.de/
> >>>
> >>>
> 
> >>>
> >>>
> >>> To unsubscribe from the CCP4BB list, click the following link:
> >>> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1
> >>>
> >>> This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a
> >>> mailing list hosted by www.jiscmail.ac.uk, terms & conditions are
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> >
> >
> >
>
> 
>
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[ccp4bb] Postdoctoral Scholarship Opportunity in the Lane group (PBIO) CFEL/DESY, Hamburg, Germany

[TJ Lane]

Uncovering protein dynamics: developing statistical crystallography
Postdoctoral Scholarship

Cluster of Excellence: Advanced Imaging of Matter

Center for Free Electron Laser Science (CFEL), Hamburg, Germany

Protein motion is at the heart of life, but remains challenging to witness 
firsthand. Proteins move to reading and copying DNA, convert sunlight into 
chemical energy, and transmit signals between cells, yet our ability to image 
these functions is limited by the technology we have to image protein dynamics.

A new opportunity to meet this challenge is emerging, however, as 
crystallographic methods advance. We can now collect data from thousands to 
millions of crystals of the same protein, and it is clear no two crystals are 
identical. Thus, analyzing their distribution may reveal protein structural 
fluctuations directly. Can we develop a statistical crystallography to turn 
these large datasets into knowledge about protein dynamics? Can we use 
statistical analysis of crystal-to-crystal variation to separate signal from 
noise in time resolved experiments, revealing protein motion induced by mixing 
with a ligand or excitation with a pump laser? We seek a computational 
scientist who will answer these questions.

As the ideal candidate, you will have the following key attributes:

* A doctoral degree in physics, biophysics, chemistry, applied mathematics or a 
related field
* Working knowledge of macromolecular crystallographic methods and software
* A track record of scientific computing and programming
* The ability to drive your work independently and show initiative

And you will be excited to take on the following responsibilities:

* Mathematical modeling of protein dynamics and protein crystal diffraction data
* Interpretation and analysis of experimental results
* Open-source development to make your insights available to the broader 
scientific community
* Presenting your work in written and oral form to the scientific community
* Helping to foster an environment conductive to collaboration, learning, 
growth, and fun

Available immediately is a two-year contract with a competitive salary and the 
opportunity to work and live in the beautiful and vibrant city of Hamburg, 
Germany. The group is housed at the Center for Free Electron Laser Science on 
the vibrant DESY campus (Luruper Chaussee 149, 22761 Hamburg, Germany).

This position is funded through the Advanced Imaging of Matter Cluster of 
Excellence (https://www.cui-advanced.uni-hamburg.de/en.html). The successful 
candidate will become a fellow of the cluster and have access to career 
support, training opportunities, and an engaging scientific network via the 
cluster. The candidate will have additional technical and mentorship support 
from partners Andrea Thorne, Arwen Pearson, and Nicholas Pearce and their 
groups during the course of the project.

We are an equal opportunity employer. Women and minorities are encouraged to 
apply and handicapped persons will be preferred given equal qualifications.

Application deadline: 17 June 2021
Applications should include a cover letter, a tabular curriculum vitae, and 
copies of degree certificate(s). Please send applications by email to: 


For more details, write directly to TJ Lane: 


Cross-Posting on the AIM Cluster of Excellence page (funding source for this 
project): 
https://www.uni-hamburg.de/stellenangebote/ausschreibung.html?jobID=4c3ff9d2b6bcfbed3ee252108b0e5164fab487a9



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[ccp4bb] Postdoctoral Fellow Position at UT Southwestern Medical Center

[Xin Liu]

Postdoctoral Fellow Position at UT Southwestern Medical Center
on ‘Chromatin Structure and Epigenetics’

Dear All,

A Postdoctoral Fellow position is immediately available for a highly motivated 
individual in Dr. Xin Liu’s group at UT Southwestern Medical Center. Research 
in the Liu Lab is focused on structure and function of large macromolecular 
assemblies involved in formation of heritable high-order chromatin structure. 
To achieve the research goal, the Liu lab leverages a combination of advanced 
tools, including biochemical reconstitution (e.g. protein-protein, 
protein-nucleosome, or protein-non-coding RNA complexes), X-ray 
crystallography, cryo-EM, proteomics, and genomics by next-generation 
sequencing. The Liu lab also routinely uses a variety of cancer cells and 
pluripotent stem cells to perform structure-based functional studies. 
Additional information is available here: 
http://profiles.utsouthwestern.edu/profile/127166/xin-liu.html

Applicants with demonstrated expertise in structural biology and in particular 
cryo-EM, protein and nucleic acid biochemistry, or chromatin biology are 
encouraged to apply.  Candidates must hold a Ph.D. and/or M.D. degree with 
first author publications and should submit a CV and contact information of 2-3 
references to the PI by e-mail at: 
xin@utsouthwestern.edu

Best wishes,
Xin Liu
---
Xin Liu, Ph.D.
Associate Professor
Cecil H. and Ida Green Center for
Reproductive Biology Sciences
UT Southwestern Medical Center
5323 Harry Hines Blvd., J7.104A
Dallas, TX 75390-8511
Phone: 214-648-2493
Fax: 214-648-0383
E-mail: xin@utsouthwestern.edu



UT Southwestern

Medical Center

The future of medicine, today.



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Re: [ccp4bb] How can I refine the occupancy with Refmac?

[James Holton]

I wrote a script for auto-generating occupancy refinement relationships 
for refmac. It is perhaps not as sophisticated as what phenix does 
internally, but it gets common things right, like if you have a 2-headed 
side chains or partial-occupancy metals.


https://bl831.als.lbl.gov/~jamesh/scripts/refmac_occupancy_setup.com

It is a csh/awk shell script. You run it with the name of your input pdb 
file on the command line and it dumps the refmac keywords to a file 
called refmac_opts_occ.txt and to the terminal.  If you want every 
"hetatm" atom to be refined put "allhet" as a second command-line argument.


You can then perhaps copy-and-paste this to the relevant GUI window.

-James Holton
MAD Scientist

On 6/7/2021 3:35 AM, Marina Gárdonyi wrote:

Hi,

I didn't know that I can also enter keywords without a file! That is a 
good note, thank you!!


Best regards,
Marina

Zitat von Jon Cooper :

Hello, the keywords can be entered in refmac gui (in one of the 
dropdown things), so you don't need a file, as such, but it's useful 
to keep a record.


Sent from ProtonMail mobile

 Original Message 
On 5 Jun 2021, 16:39, Marina Gárdonyi wrote:


Hello everyone,

I am trying to refine structures with Refmac.

The problem is that nobody from my working group is familiar with this
programm. They are using Phenix exclusively.

That's why I need your help. I would like to refine the occupancy. I
know that I need a keyword file for this, but I have no idea how to
create such a keyword file.

Can someone maybe send me a sample file? I think that would help me. I
have found essential keywords, but I don't know how to build up such a
file.

Thank you very much in advance!

Best regards,
Marina

--
Marina Gárdonyi

PhD Student, Research Group Professor Dr. Klebe

Department of Pharmaceutical Chemistry

Philipps-University Marburg

Marbacher Weg 6, 35032 Marburg, Germany

Phone: +49 6421 28 21392

E-Mail: marina@pharmazie.uni-marburg.de

http://www.agklebe.de/

 



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[ccp4bb] self-inhibitory fragments from a protein

[Andrew Lovering]

Dear CCP4bb,

I have a partly specific question, relating to multidomain enzymes.

We have a nice hi-res structure of an enzyme, which comprises domain A 
(non-catalytic) and domain B (enzymatic)

Through the magic of unstimulated in-tray proteolysis, there are one and a half 
copies in the ASU, one full AB and one A half

The second (proteolysed) domain A is jammed in active site B of the full-length 
AB copy. The contacts look glorious and specific but we don't have enough 
sequence variation among homologues to say if interfaces are conserved.

A full-length AB protein would never inhibit another because the AB domain 
arrangement would cause a steric clash.

So my question is - "do you know of any enzyme where it gets proteolysed to 
generate a fragment that goes on to inhibit itself?". I'd prefer answers that 
are about fragments/complexes rather than say a zymogen state. Hope that makes 
sense

Be great if you can tell me of any - not the easiest thing to search for!
Thanks in advance
Andy



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[ccp4bb] Protein Crystallisation Position at Vernalis Research, Cambridge, UK (full-time, permanent)

[Lisa Baker]

Protein Crystallisation Position at Vernalis Research, Cambridge, UK  
(full-time, permanent)



Vernalis Research (based at Granta Park in Cambridge, UK) has a new full-time 
position for a Scientist to join our Biophysics group. Working in collaboration 
with an integrated team of protein scientists, NMR spectroscopists and 
Crystallographers, your primary role will be the discovery and optimisation of 
protein crystals for X-ray data collection and analysis, supporting multiple 
projects ranging from target validation to lead optimisation.



This post would suit someone with a practical understanding of protein 
biochemistry and laboratory-based research experience, preferably gained within 
a drug discovery environment. Applications will be considered from graduates 
with a BSc/MSc in Biochemistry, Biophysics, Chemistry, Molecular Biology or 
other related discipline, as well as candidates with an equivalent level of 
work experience.



Further details about the position can be found on our website:



Scientist, Crystallisation | Vernalis 
Research



And on LinkedIn:



https://www.linkedin.com/​jobs/view/2586994587/



To apply, send your CV and a covering email to 
h...@vernalis.com



We are looking forward to hearing from you.



With best wishes,



Lisa Baker BSc (Hons), PhD.

Vernalis (R) Limited

Granta Park

Great Abington

Cambridge

CB21 6GB

United Kingdom





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If verification of this email is sought then please request a hard copy. Unless 
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Granta Park, Great Abington
Cambridge, CB21 6GB, United Kingdom
Tel: +44 (0)1223 895 555




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Re: [ccp4bb] H-bond modelling

[Edwin Pozharski]

There are tools such as hbplus

https://www.ebi.ac.uk/thornton-srv/software/HBPLUS/

that will calculate predicted hydrogen bonds (including ligands, you just
need to define their chemistry) throughout a structure. These tools usually
go beyond simple distance considerations and analyze further geometry
details.

Proper donor acceptor pair at 2.6A is almost without doubt a hydrogen bond.

On Sun, Jun 6, 2021, 4:17 PM vivek sharma  wrote:

> Dear all,
>
> How does one validate if 2 atoms (non-hydrogen) which are close enough,
> actually participate in H-bonding?
> I have a recent data that i am currently working on, the NH2 of ARG is at
> 2.61A from ligand's oxygen atom (refer to attached image), since there is
> no electron density for hydrogens, how can i be sure if i am modelling
> ligand correctly?
>
> thanks
>
> --
>
> To unsubscribe from the CCP4BB list, click the following link:
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Re: [ccp4bb] How can I refine the occupancy with Refmac?

[Eleanor Dodson]

In GUI2 the Advanced box allows you to do that
eg RESO 10 3 say if you wanted to restrict resolution..
Eleanor

On Mon, 7 Jun 2021 at 11:35, Marina Gárdonyi <
marina@pharmazie.uni-marburg.de> wrote:

> Hi,
>
> I didn't know that I can also enter keywords without a file! That is a
> good note, thank you!!
>
> Best regards,
> Marina
>
> Zitat von Jon Cooper :
>
> > Hello, the keywords can be entered in refmac gui (in one of the
> > dropdown things), so you don't need a file, as such, but it's useful
> > to keep a record.
> >
> > Sent from ProtonMail mobile
> >
> >  Original Message 
> > On 5 Jun 2021, 16:39, Marina Gárdonyi wrote:
> >
> >> Hello everyone,
> >>
> >> I am trying to refine structures with Refmac.
> >>
> >> The problem is that nobody from my working group is familiar with this
> >> programm. They are using Phenix exclusively.
> >>
> >> That's why I need your help. I would like to refine the occupancy. I
> >> know that I need a keyword file for this, but I have no idea how to
> >> create such a keyword file.
> >>
> >> Can someone maybe send me a sample file? I think that would help me. I
> >> have found essential keywords, but I don't know how to build up such a
> >> file.
> >>
> >> Thank you very much in advance!
> >>
> >> Best regards,
> >> Marina
> >>
> >> --
> >> Marina Gárdonyi
> >>
> >> PhD Student, Research Group Professor Dr. Klebe
> >>
> >> Department of Pharmaceutical Chemistry
> >>
> >> Philipps-University Marburg
> >>
> >> Marbacher Weg 6, 35032 Marburg, Germany
> >>
> >> Phone: +49 6421 28 21392
> >>
> >> E-Mail: marina@pharmazie.uni-marburg.de
> >>
> >> http://www.agklebe.de/
> >>
> >> 
> >>
> >> To unsubscribe from the CCP4BB list, click the following link:
> >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1
> >>
> >> This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a
> >> mailing list hosted by www.jiscmail.ac.uk, terms & conditions are
> >> available at https://www.jiscmail.ac.uk/policyandsecurity/
>
>
>
> --
> Marina Gárdonyi
>
> PhD Student, Research Group Professor Dr. Klebe
>
> Department of Pharmaceutical Chemistry
>
> Philipps-University Marburg
>
> Marbacher Weg 6, 35032 Marburg, Germany
>
> Phone: +49 6421 28 21392
>
> E-Mail: marina@pharmazie.uni-marburg.de
>
> http://www.agklebe.de/
>
> 
>
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1
>
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Re: [ccp4bb] How can I refine the occupancy with Refmac?

[Marina Gárdonyi]

Hi,

I didn't know that I can also enter keywords without a file! That is a  
good note, thank you!!


Best regards,
Marina

Zitat von Jon Cooper :

Hello, the keywords can be entered in refmac gui (in one of the  
dropdown things), so you don't need a file, as such, but it's useful  
to keep a record.


Sent from ProtonMail mobile

 Original Message 
On 5 Jun 2021, 16:39, Marina Gárdonyi wrote:


Hello everyone,

I am trying to refine structures with Refmac.

The problem is that nobody from my working group is familiar with this
programm. They are using Phenix exclusively.

That's why I need your help. I would like to refine the occupancy. I
know that I need a keyword file for this, but I have no idea how to
create such a keyword file.

Can someone maybe send me a sample file? I think that would help me. I
have found essential keywords, but I don't know how to build up such a
file.

Thank you very much in advance!

Best regards,
Marina

--
Marina Gárdonyi

PhD Student, Research Group Professor Dr. Klebe

Department of Pharmaceutical Chemistry

Philipps-University Marburg

Marbacher Weg 6, 35032 Marburg, Germany

Phone: +49 6421 28 21392

E-Mail: marina@pharmazie.uni-marburg.de

http://www.agklebe.de/



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--
Marina Gárdonyi

PhD Student, Research Group Professor Dr. Klebe

Department of Pharmaceutical Chemistry

Philipps-University Marburg

Marbacher Weg 6, 35032 Marburg, Germany

Phone: +49 6421 28 21392

E-Mail: marina@pharmazie.uni-marburg.de

http://www.agklebe.de/



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Re: [ccp4bb] H-bond modelling

[Paul Emsley]

On Mon, 2021-06-07 at 01:35 +0530, vivek sharma wrote:
> 
> How does one validate if 2 atoms (non-hydrogen) which are close enough, 
> actually participate in H-bonding?
> I have a recent data that i am currently working on, the NH2 of ARG is at 
> 2.61A from ligand's oxygen atom (refer to
> attached image), since there is no electron density for hydrogens, how can i 
> be sure if i am modelling ligand
> correctly? 
> 

This doesn't answer your question, but the difference map suggests radiation 
damage to the very site for which you
require optimal clarity. Less dose, more crystals?

Paul.



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[ccp4bb] Register now for Instruct-ERIC ‘Structure Meets Function’ webinar – 8 June

[Claudia Alén Amaro]

Registration is now open for the 11h webinar in the series, which will be 
hosted by Instruct Centre France 2 on 8 June 2021

[Instruct-ERIC logo]

[Instruct-ERIC]   [LinkedIn] 
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Dear All

Featuring expert speakers from Instruct Centres across Europe, the 
Instruct-ERIC webinar series, Structure Meets 
Function, highlights some of the 
latest developments in structural biology, demonstrating how integrative 
methods are enabling scientists to decipher the mechanisms that underpin health 
and disease.



Register now

https://bit.ly/3w1y6r4




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Registration is open for the 11th 
webinar in the series, which will be hosted by Instruct Centre France 2 
 on 8 June 2021, 11:00 - 12:30 CEST. 
The upcoming webinar will feature talks on the application of structural 
biology methods, including cryo-electron microscopy and structural dynamics, to 
better understand key aspects of protein complexes structure and interactions.



Talk 1: DC-SIGN and L-SIGN receptors from immune cells recognize spike 
glycoprotein and promote SARS CoV-2 trans infection
Speaker:Franck Fieschi



Talk 2: Structural dynamics of a newly discovered photoenzyme
Speaker: Martin Weik



Talk 3: The high resolution cryo-EM structure of full-length La Crosse virus 
polymerase reveals functionally important conformational changes
Speaker: Helene Malet



Moderator: Darren Hart, IBS Grenoble



You can view the full abstracts and register 
 on the Instruct-ERIC website, where 
you can also watch recordings of the previous 
webinars.



Questions can be submitted in advance of the webinar by sending them to 
paul...@instruct-eric.org.



We hope that you can join the webinar - please feel free to share this message 
with anyone who might be interested in attending.



Best wishes,

Instruct-ERIC Hub Team 







Dr Claudia Alén Amaro
Senior Project Manager

[instructlogo]

Instruct-ERIC, Oxford House, Parkway Court, John Smith Drive, Oxford, OX4 2JY, 
UK.
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