subject:"\[Freesurfer\] Cortical thickness"

Re: [Freesurfer] Cortical Thickness Measurement in Cortical Lesion ROI

2024-01-01 Thread Douglas N. Greve

You should load 
$chronic_CMI_volume_path/"$sub_base"_chronic-CMI_4-1.nii.gz into 
freeview with the surfaces, eg

cd subject/mri
freeview nu.mgz 
$chronic_CMI_volume_path/"$sub_base"_chronic-CMI_4-1.nii.gz -f 
../surfs/?h.white ../surfs/?h.pial

and see if the lesion maps between the white and pial surfaces
For vol2surf, use --projfrac-max
Also, make sure that the freeview threshold is not so high that it will 
exclude your segmentation



On 12/7/2023 5:22 AM, 李浩 wrote:


External Email - Use Caution

Dear Freesurfer Experts,

 I am currently engaged in a project where I need to measure the 
cortical thickness of a segmented cortical lesion (ROI) in the volume 
space.
After running recon-all for my subjects, i employed 'mri_vol2surf' 
function to project my ROI from the native T1 space to indiviaul 
surface space. My command was as follows:


mri_vol2surf —mov 
 $chronic_CMI_volume_path/"$sub_base"_chronic-CMI_4-1.nii.gz --hemi rh 
--surf white --projfrac-avg -0.1 1 0.05 --interp nearest --o 
"$sub"_chronic-CMI_4-1_rh.mgh --regheader "$sub" --trgsubject “$sub”


However, I encountered an issue where I could not locate the ROI in 
the individual surface space, as the values in 
"$sub"_chronic-CMI_4-1_rh.mgh were all zero.


I suspect this might be due to a registration error or possibly the 
small size of my ROI (only 4 voxels). Do you have any suggestions on 
how to address this issue?


Additionally, I am wondering whether it's possible to register the 
cortical thickness map from the surface space back to the individual 
volume space, then calculate the mean cortical thickness of my ROI 
there. Alternatively, is it feasible to convert the coordinates of my 
ROI's center point to the MNI152 coordinate and then surface-based 
coordinates and directly create an ROI in the surface space?


Any feedback on these questions would be greatly appreciated. Thanks 
in advance.


Bests,
Hao Li


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[Freesurfer] Cortical Thickness Measurement in Cortical Lesion ROI

2023-12-07 Thread 李浩

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Dear Freesurfer Experts, 


 I am currently engaged in a project where I need to measure the cortical 
thickness of a segmented cortical lesion (ROI) in the volume space. 
 
After running recon-all for my subjects, i employed 'mri_vol2surf' function to 
project my ROI from the native T1 space to indiviaul surface space. My command 
was as follows:


mri_vol2surf —mov  $chronic_CMI_volume_path/"$sub_base"_chronic-CMI_4-1.nii.gz 
--hemi rh --surf white --projfrac-avg -0.1 1 0.05 --interp nearest --o 
"$sub"_chronic-CMI_4-1_rh.mgh --regheader "$sub" --trgsubject “$sub”


However, I encountered an issue where I could not locate the ROI in the 
individual surface space, as the values in "$sub"_chronic-CMI_4-1_rh.mgh were 
all zero.


I suspect this might be due to a registration error or possibly the small size 
of my ROI (only 4 voxels). Do you have any suggestions on how to address this 
issue?


Additionally, I am wondering whether it's possible to register the cortical 
thickness map from the surface space back to the individual volume space, then 
calculate the mean cortical thickness of my ROI there. Alternatively, is it 
feasible to convert the coordinates of my ROI's center point to the MNI152 
coordinate and then surface-based coordinates and directly create an ROI in the 
surface space?


Any feedback on these questions would be greatly appreciated. Thanks in advance.


Bests,
Hao Li

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continue to communicate over unencrypted e-mail.

[Freesurfer] Cortical Thickness Measurement in Cortical Lesion ROI

2023-12-07 Thread 李浩

External Email - Use Caution

Dear Freesurfer Experts, 


 I am currently engaged in a project where I need to measure the cortical 
thickness of a segmented cortical lesion (ROI) in the volume space. 
 
After running recon-all for my subjects, i employed 'mri_vol2surf' function to 
project my ROI from the native T1 space to indiviaul surface space to project 
the ROI from the native T1 space to the individual surface space. My command 
was as follows:


$chronic_CMI_volume_path/"$sub_base"_chronic-CMI_4-1.nii.gz --hemi rh --surf 
white --projfrac-avg -0.1 1 0.05 --interp nearest --o 
"$sub"_chronic-CMI_4-1_rh.mgh --regheader "$sub" --trgsubject “$sub”


However, I encountered an issue where I could not locate the ROI in the 
individual surface space, as the values in "$sub"_chronic-CMI_4-1_rh.mgh were 
all zero.


I suspect this might be due to a registration error or possibly the small size 
of my ROI (only 4 voxels). Do you have any suggestions on how to address this 
issue?


Additionally, I am wondering whether it's possible to register the cortical 
thickness map from the surface space back to the individual volume space, then 
calculate the mean cortical thickness of my ROI there. Alternatively, is it 
feasible to convert the coordinates of my ROI's center point to the MNI152 
coordinate and then surface-based coordinates and directly create an ROI in the 
surface space?


Any feedback on these questions would be greatly appreciated. Thanks in advance.


Bests,
Hao Li




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continue communication over unencrypted e-mail, please notify the sender of 
this message immediately.  Continuing to send or respond to e-mail after 
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continue to communicate over unencrypted e-mail.

Re: [Freesurfer] Cortical thickness of elephant brain

2023-05-25 Thread Malav Shah

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Dear Douglas, 
using .mgz worked well. 
As the command is supposed to generate a "curvature" file, I assumed it to be a 
surface format. 
Thanks a bunch. 

with regards, 
Malav 

From: "Douglas N. Greve"  
To: "freesurfer"  
Sent: Wednesday, May 24, 2023 3:46:35 PM 
Subject: Re: [Freesurfer] Cortical thickness of elephant brain 



External Email - Use Caution 

Can you try using lh.thickness.mgz instead of just lh.thickness? 

On 5/23/2023 1:12 PM, Malav Shah wrote: 




External Email - Use Caution 
Hi there, 
I believe the problem occured, because I generated pial surface by segmenting, 
and then tessellating that label volume, instead the way I see in log file of 
the subject 'bert' where standard autorecon achieves it using the 
'mris_place_surface' command 
(mris_place_surface --adgws-in ../surf/autodet.gw.stats.rh.dat --seg 
aseg.presurf.mgz --threads 8 --wm wm.mgz --invol brain.finalsurfs.mgz --rh --i 
../surf/rh.white --o ../surf/rh.pial.T1 --pial --nsmooth 0 --rip-label 
../label/rh.cortex+hipamyg.label --pin-medial-wall ../label/rh.cortex.label 
--aparc ../label/rh.aparc.annot --repulse-surf ../surf/rh.white --white-surf 
../surf/rh.white) 

Therefore, to go that way, I tried to "cheat" freesurfer with 
"mris_place_surface --adgws-in autodet.gw.stats.lh.dat --invol vol_seg.nii.gz 
--lh --i lh.wm.5smth --o lh.pial.nofix --pial --repulse-surf lh.wm.5smth" 
command using (following) made-up files 
- vol_seg.nii.gz- segmentation volume with voxel-intensities 1 (pial-matter), 2 
(gray matter) and 3 (white matter) 
- lh.wm.smth- white matter surface generated using white matter segmentation's 
tessellation, and then smootheed with n_smooth = 5 
- autodet.gw.stats.lh.pial is duplicated from the subject "bert" file with 
following values 

hemicode 1 
white_border_hi 3 
white_border_low 3 
white_outside_low 3 
white_inside_hi 3 
white_outside_hi 3 
pial_border_hi 1 
pial_border_low 1 
pial_outside_low 1 
pial_inside_hi 1 
pial_outside_hi 1 
use_mode 1 
variablesigma 1.00 
std_scale 1.00 
adWHITE_MATTER_MEAN 3 
MAX_WHITE 3.0 
MIN_BORDER_WHITE 2.50 
MAX_BORDER_WHITE 2.500 
MAX_GRAY 2.00 
MID_GRAY 2.00 
MIN_GRAY_AT_CSF_BORDER 1.500 
MAX_GRAY_AT_CSF_BORDER 1.500 
MIN_CSF 0.00 
adMAX_CSF 1.000 
white_mean 3.00 
white_mode 3.00 
white_std 0 
gray_mean 2.00 
gray_mode 2.00 
gray_std 0 
min_border_white 2.00 
max_border_white 3.0 
min_gray_at_white_border 1.5 
max_gray 2.00 
min_gray_at_csf_border 1.500 
max_gray_at_csf_border 2.44 
min_csf 1.00 
max_csf 1.00 
max_gray_scale 0.00 
max_scale_down 0.20 

However...I am getting the segmenetation fault error when it reads the surface 

cd 
/Volumes/experiment/share/Malav/Ele_ana_cortexMRI/pipelines/dumba_piece/fs_trl2 
setenv SUBJECTS_DIR /Applications/freesurfer/7.2.0/subjects/ 
mris_place_surface --adgws-in autodet.gw.stats.lh.dat --invol vol_seg.nii.gz 
--lh --i lh.wm.5smth --o lh.pial.nofix --pial --repulse-surf lh.wm.5smth 

Reading in input surface lh.wm.5smth 
ERROR: MRISedges(): too many faces: 181 938 n=7, m=3, k=2 
Segmentation fault: 11 

Any suggestions? 
Thanks in advance, 
malav 

From: "Malav Shah" [ mailto:malav.s...@bccn-berlin.de | 
 ] 
To: "Freesurfer support list" [ mailto:freesurfer@nmr.mgh.harvard.edu | 
 ] 
Sent: Monday, May 22, 2023 5:35:56 PM 
Subject: Re: [Freesurfer] Cortical thickness of elephant brain 



External Email - Use Caution 
Thank you for a swift reply, Doug. 
Upon running the command "mris_diff --debug --min-dist lh.pial.5smth 
lh.wm.5smth 1 lh.thickness", the command line throws error that it can't find 
file. 
outcome: 

5 --min-dist 
Use Exact = 1 
Writing mindist to lh.thickness 
error: unknown file type for file (lh.thickness) 
mris_diff done 

I tried with an absolute file path which also didn't work. 
Strangely, when mentioned no file location, but giving in a directory 
"mris_diff --debug --min-dist lh.pial.5smth lh.wm.5smth 1 
/Users/user-name/Desktop/", 
2 files namely COR-info and COR-001 are created on Desktop, and COR-info can be 
loaded as 'overlay' for a surface. Unfortunately, these curvature values are 
not within range at all. 

For further details, using the command with --debug flag gives the following 
outcome (on command line) 

Use Exact = 0 
Writing mindist to /Users/monster/Desktop/ 
non-standard value for imnr1 (1, usually 256) in volume structure 
non-standard value for type (3, usually 0) in volume structure 
non-standard value for width (177753, usually 256) in volume structure 
non-standard value for height (1, usually 256) in volume structure 
mris_diff done 

Here I attach a couple of screenshots to elaborate the outcome. 
Kindly let me know how I can rectify the issue. 

Thanks and regards, 
malav 

From: "Douglas N. Greve" [ mailto:dgr...@mgh.harvard.ed

Re: [Freesurfer] Cortical thickness of elephant brain

2023-05-24 Thread Douglas N. Greve

   External Email - Use Caution


Can you try using lh.thickness.mgz instead of just lh.thickness?

On 5/23/2023 1:12 PM, Malav Shah wrote:


External Email - Use Caution

Hi there,
I believe the problem occured, because I generated pial surface by 
segmenting, and then tessellating that label volume, instead the way I 
see in log file of the subject 'bert' where standard autorecon 
achieves it using the 'mris_place_surface' command
(mris_place_surface --adgws-in ../surf/autodet.gw.stats.rh.dat --seg 
aseg.presurf.mgz --threads 8 --wm wm.mgz --invol brain.finalsurfs.mgz 
--rh --i ../surf/rh.white --o ../surf/rh.pial.T1 --pial --nsmooth 0 
--rip-label ../label/rh.cortex+hipamyg.label --pin-medial-wall 
../label/rh.cortex.label --aparc ../label/rh.aparc.annot 
--repulse-surf ../surf/rh.white --white-surf ../surf/rh.white)


Therefore, to go that way, I tried to "cheat" freesurfer with
"mris_place_surface --adgws-in autodet.gw.stats.lh.dat --invol 
vol_seg.nii.gz --lh --i lh.wm.5smth --o lh.pial.nofix --pial 
--repulse-surf lh.wm.5smth"

command using (following) made-up files
- vol_seg.nii.gz- segmentation volume with voxel-intensities 1 
(pial-matter), 2 (gray matter) and 3 (white matter)
- lh.wm.smth- white matter surface generated using white matter 
segmentation's tessellation, and then smootheed with n_smooth = 5
- autodet.gw.stats.lh.pial is duplicated from the subject "bert" file 
with following values


hemicode   1
white_border_hi    3
white_border_low   3
white_outside_low  3
white_inside_hi    3
white_outside_hi   3
pial_border_hi    1
pial_border_low   1
pial_outside_low  1
pial_inside_hi    1
pial_outside_hi   1
use_mode 1
variablesigma 1.00
std_scale 1.00
adWHITE_MATTER_MEAN 3
MAX_WHITE 3.0
MIN_BORDER_WHITE 2.50
MAX_BORDER_WHITE 2.500
MAX_GRAY 2.00
MID_GRAY 2.00
MIN_GRAY_AT_CSF_BORDER 1.500
MAX_GRAY_AT_CSF_BORDER 1.500
MIN_CSF 0.00
adMAX_CSF 1.000
white_mean 3.00
white_mode 3.00
white_std 0
gray_mean 2.00
gray_mode 2.00
gray_std 0
min_border_white 2.00
max_border_white 3.0
min_gray_at_white_border 1.5
max_gray 2.00
min_gray_at_csf_border 1.500
max_gray_at_csf_border 2.44
min_csf 1.00
max_csf 1.00
max_gray_scale 0.00
max_scale_down 0.20

However...I am getting the segmenetation fault error when it reads the 
surface


cd 
/Volumes/experiment/share/Malav/Ele_ana_cortexMRI/pipelines/dumba_piece/fs_trl2

setenv SUBJECTS_DIR /Applications/freesurfer/7.2.0/subjects/
mris_place_surface --adgws-in autodet.gw.stats.lh.dat --invol 
vol_seg.nii.gz --lh --i lh.wm.5smth --o lh.pial.nofix --pial 
--repulse-surf lh.wm.5smth


Reading in input surface lh.wm.5smth
ERROR: MRISedges(): too many faces: 181 938 n=7, m=3, k=2
Segmentation fault: 11

Any suggestions?
Thanks in advance,
malav

*From: *"Malav Shah" 
*To: *"Freesurfer support list" 
*Sent: *Monday, May 22, 2023 5:35:56 PM
*Subject: *Re: [Freesurfer] Cortical thickness of elephant brain

External Email - Use Caution

Thank you for a swift reply, Doug.
Upon running the command "mris_diff --debug --min-dist lh.pial.5smth 
lh.wm.5smth 1 lh.thickness", the command line throws error that it 
can't find file.

outcome:

5 --min-dist
Use Exact = 1
Writing mindist to lh.thickness
error: unknown file type for file (lh.thickness)
mris_diff done

I tried with an absolute file path which also didn't work.
Strangely, when mentioned no file location, but giving in a directory 
"mris_diff --debug --min-dist lh.pial.5smth lh.wm.5smth 1 
/Users/user-name/Desktop/",
2 files namely COR-info and COR-001  are created on Desktop, and 
COR-info can be loaded as 'overlay' for a surface. Unfortunately, 
these curvature values are not within range at all.


For further details, using the command with --debug flag gives the 
following outcome (on command line)


Use Exact = 0
Writing mindist to /Users/monster/Desktop/
non-standard value for imnr1 (1, usually 256) in volume structure
non-standard value for type (3, usually 0) in volume structure
non-standard value for width (177753, usually 256) in volume structure
non-standard value for height (1, usually 256) in volume structure
mris_diff done

Here I attach a couple of screenshots to elaborate the outcome.
Kindly let me know how I can rectify the issue.

Thanks and regards,
malav

*From: *"Douglas N. Greve" 
*To: *"freesurfer" 
*Sent: *Monday, May 22, 2023 3:32:28 PM
*Subject: *Re: [Freesurfer] Cortical thickness of elephant brain

External Email - Use Caution

Hi Malav, try
mris_diff --min-dist lh.wm.smth5 lh.pial.smth5 1 lh.thickness
The output will be on the pial surface. Reverse the surface order to 
get it on the white surface

doug


On 5/22/2023 5:48 AM, Malav

Re: [Freesurfer] Cortical thickness of elephant brain

2023-05-23 Thread Malav Shah

External Email - Use Caution

Hi there, 
I believe the problem occured, because I generated pial surface by segmenting, 
and then tessellating that label volume, instead the way I see in log file of 
the subject 'bert' where standard autorecon achieves it using the 
'mris_place_surface' command 
(mris_place_surface --adgws-in ../surf/autodet.gw.stats.rh.dat --seg 
aseg.presurf.mgz --threads 8 --wm wm.mgz --invol brain.finalsurfs.mgz --rh --i 
../surf/rh.white --o ../surf/rh.pial.T1 --pial --nsmooth 0 --rip-label 
../label/rh.cortex+hipamyg.label --pin-medial-wall ../label/rh.cortex.label 
--aparc ../label/rh.aparc.annot --repulse-surf ../surf/rh.white --white-surf 
../surf/rh.white) 

Therefore, to go that way, I tried to "cheat" freesurfer with 
"mris_place_surface --adgws-in autodet.gw.stats.lh.dat --invol vol_seg.nii.gz 
--lh --i lh.wm.5smth --o lh.pial.nofix --pial --repulse-surf lh.wm.5smth" 
command using (following) made-up files 
- vol_seg.nii.gz- segmentation volume with voxel-intensities 1 (pial-matter), 2 
(gray matter) and 3 (white matter) 
- lh.wm.smth- white matter surface generated using white matter segmentation's 
tessellation, and then smootheed with n_smooth = 5 
- autodet.gw.stats.lh.pial is duplicated from the subject "bert" file with 
following values 

hemicode 1 
white_border_hi 3 
white_border_low 3 
white_outside_low 3 
white_inside_hi 3 
white_outside_hi 3 
pial_border_hi 1 
pial_border_low 1 
pial_outside_low 1 
pial_inside_hi 1 
pial_outside_hi 1 
use_mode 1 
variablesigma 1.00 
std_scale 1.00 
adWHITE_MATTER_MEAN 3 
MAX_WHITE 3.0 
MIN_BORDER_WHITE 2.50 
MAX_BORDER_WHITE 2.500 
MAX_GRAY 2.00 
MID_GRAY 2.00 
MIN_GRAY_AT_CSF_BORDER 1.500 
MAX_GRAY_AT_CSF_BORDER 1.500 
MIN_CSF 0.00 
adMAX_CSF 1.000 
white_mean 3.00 
white_mode 3.00 
white_std 0 
gray_mean 2.00 
gray_mode 2.00 
gray_std 0 
min_border_white 2.00 
max_border_white 3.0 
min_gray_at_white_border 1.5 
max_gray 2.00 
min_gray_at_csf_border 1.500 
max_gray_at_csf_border 2.44 
min_csf 1.00 
max_csf 1.00 
max_gray_scale 0.00 
max_scale_down 0.20 

However...I am getting the segmenetation fault error when it reads the surface 

cd 
/Volumes/experiment/share/Malav/Ele_ana_cortexMRI/pipelines/dumba_piece/fs_trl2 
setenv SUBJECTS_DIR /Applications/freesurfer/7.2.0/subjects/ 
mris_place_surface --adgws-in autodet.gw.stats.lh.dat --invol vol_seg.nii.gz 
--lh --i lh.wm.5smth --o lh.pial.nofix --pial --repulse-surf lh.wm.5smth 

Reading in input surface lh.wm.5smth 
ERROR: MRISedges(): too many faces: 181 938 n=7, m=3, k=2 
Segmentation fault: 11 

Any suggestions? 
Thanks in advance, 
malav 

From: "Malav Shah"  
To: "Freesurfer support list"  
Sent: Monday, May 22, 2023 5:35:56 PM 
Subject: Re: [Freesurfer] Cortical thickness of elephant brain 



External Email - Use Caution 


Thank you for a swift reply, Doug. 
Upon running the command "mris_diff --debug --min-dist lh.pial.5smth 
lh.wm.5smth 1 lh.thickness", the command line throws error that it can't find 
file. 
outcome: 

5 --min-dist 
Use Exact = 1 
Writing mindist to lh.thickness 
error: unknown file type for file (lh.thickness) 
mris_diff done 

I tried with an absolute file path which also didn't work. 
Strangely, when mentioned no file location, but giving in a directory 
"mris_diff --debug --min-dist lh.pial.5smth lh.wm.5smth 1 
/Users/user-name/Desktop/", 
2 files namely COR-info and COR-001 are created on Desktop, and COR-info can be 
loaded as 'overlay' for a surface. Unfortunately, these curvature values are 
not within range at all. 

For further details, using the command with --debug flag gives the following 
outcome (on command line) 

Use Exact = 0 
Writing mindist to /Users/monster/Desktop/ 
non-standard value for imnr1 (1, usually 256) in volume structure 
non-standard value for type (3, usually 0) in volume structure 
non-standard value for width (177753, usually 256) in volume structure 
non-standard value for height (1, usually 256) in volume structure 
mris_diff done 

Here I attach a couple of screenshots to elaborate the outcome. 
Kindly let me know how I can rectify the issue. 

Thanks and regards, 
malav 

From: "Douglas N. Greve"  
To: "freesurfer"  
Sent: Monday, May 22, 2023 3:32:28 PM 
Subject: Re: [Freesurfer] Cortical thickness of elephant brain 



External Email - Use Caution 

Hi Malav, try 
mris_diff --min-dist lh.wm.smth5 lh.pial.smth5 1 lh.thickness 
The output will be on the pial surface. Reverse the surface order to get it on 
the white surface 
doug 


On 5/22/2023 5:48 AM, Malav Shah wrote: 




External Email - Use Caution 
Dear freeSurfer team, 
I am trying to extract cortical thickness in a postmortem elephant (structural) 
MRI scan. 

I initiated the process with the segmentation of white (wm) and pial matter, 
using MATLAB image proc

Re: [Freesurfer] Cortical thickness of elephant brain

2023-05-22 Thread Douglas N. Greve

External Email - Use Caution

Hi Malav, try
mris_diff --min-dist lh.wm.smth5 lh.pial.smth5 1 lh.thickness
The output will be on the pial surface. Reverse the surface order to get
it on the white surface

doug

On 5/22/2023 5:48 AM, Malav Shah wrote:

External Email - Use Caution

Dear freeSurfer team,
I am trying to extract cortical thickness in a postmortem elephant
(structural) MRI scan.

Then, with 'mris_smooth' command, I smoothed these surfaces.
When attempted to estimate cortical thickness between these surfaces
(lh.pial.smth5 and lh.wm.smth5), I get the following error.

command: mris_place_surface --thickness lh.pial.smth5 lh.wm.smth5 20 5
lh.thickness

error: No such file or directory
error:
ERROR:
MRISreadVertexPositions(/Volumes/experiment/share/Malav/Ele_ana_cortexMRI/pipelines/dumba_piece/lh.wm.smth5):
surfaces differ. Main: 177753 verts 365096 faces, lh.wm.smth5: 145095
verts 298436 faces

Surfaces may be out-of-date

I understand there is a mismatch in the number of nodes of these
surfaces. How can I rectify this issue to get to the thickness?

Thanks and regards,
malav

Malav Shah
PhD candidate, Michael Brecht Lab,
Humboldt University zu Berlin.
(+49) 163-216-9391

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[Freesurfer] Cortical thickness of elephant brain

2023-05-22 Thread Malav Shah

External Email - Use Caution

Dear freeSurfer team,
I am trying to extract cortical thickness in a postmortem elephant (structural)
MRI scan.

I initiated the process with the segmentation of white (wm) and pial matter,
using MATLAB image processing tools.
Having satisfied white and pial matter labels, I "tesselated" these volumes to
generate pial and wm surfaces.
Then, with 'mris_smooth' command, I smoothed these surfaces.
When attempted to estimate cortical thickness between these surfaces
(lh.pial.smth5 and lh.wm.smth5), I get the following error.

command: mris_place_surface --thickness lh.pial.smth5 lh.wm.smth5 20 5
lh.thickness
error: No such file or directory
error:
ERROR:
MRISreadVertexPositions(/Volumes/experiment/share/Malav/Ele_ana_cortexMRI/pipelines/dumba_piece/lh.wm.smth5):
surfaces differ. Main: 177753 verts 365096 faces, lh.wm.smth5: 145095 verts
298436 faces
Surfaces may be out-of-date

I understand there is a mismatch in the number of nodes of these surfaces. How
can I rectify this issue to get to the thickness?
Thanks and regards,
malav

Malav Shah
PhD candidate, Michael Brecht Lab,
Humboldt University zu Berlin.
(+49) 163-216-9391
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