Re: [Histonet] IHC

[Emily Horst via Histonet]

Thanks everyone for all your suggestions! You've been very helpful!

On Thu, Aug 10, 2017 at 6:16 PM Cooper, Brian  wrote:

> See if you can get a sample of slides from Thermo Fisher.  We use the
> Superfrost Plus slides, and tissue loss just isn’t one of the problems we
> ever have here.  And like Tim Morken has said in his response, ensure
> there’s no trapped water under the section.
>
>
>
>
> https://www.fishersci.com/shop/products/shandon-colorfrost-plus-slides/6776214?searchHijack=true=6776214=RAPID=6776214CS
>
>
>
> Thanks,
>
>
>
> Brian
>
>
>
> *From:* Emily Horst [mailto:mashals...@gmail.com]
> *Sent:* Thursday, August 10, 2017 2:54 PM
>
>
> *To:* Cooper, Brian
> *Subject:* Re: [Histonet] IHC (EXTERNAL EMAIL)
>
>
>
> They are ones that were prepackaged and sent to us from Dianon (Dianon is
> not the lab I'm talking about). I have no idea what/who the slides are. I
> just ordered new charged slides from Mercedes Medical and all my invoice
> says is 45 degree, white. I haven't received them yet so I don't have a box
> with info on it.
>
>
>
> It's a problem we've been having for awhile.
>
>
>
> On Thu, Aug 10, 2017 at 5:49 PM Cooper, Brian 
> wrote:
>
> So I worked at a reference lab for yeears and years and years, and
> I can tell you that not all charged slides are created equally.  What type
> of slides are you using?
>
>
>
> Thanks,
>
>
>
> Brian
>
>
>
> *From:* Emily Horst [mailto:mashals...@gmail.com]
> *Sent:* Thursday, August 10, 2017 2:47 PM
> *To:* Cooper, Brian
> *Subject:* Re: [Histonet] IHC (EXTERNAL EMAIL)
>
>
>
> No I am not. I air dry them and then package them up.
>
>
>
> On Thu, Aug 10, 2017 at 5:46 PM Cooper, Brian 
> wrote:
>
> Are you baking the slides before sending them out?  How long, and what
> temperature?
>
> Thanks,
>
> Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
> Department of Pathology and Laboratory Medicine
> Children's Hospital Los Angeles
> 4650 Sunset Blvd MS#43- Los Angeles, CA 90027
> Ph: 323.361.3357 Pager: 213-209-0184
> bcoo...@chla.usc.edu
>
> -Original Message-
> From: Emily Horst via Histonet [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Thursday, August 10, 2017 2:42 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] IHC (EXTERNAL EMAIL)
>
> Hi everyone,
>
> We send our IHCs to an outside lab. I send them the unstained slides. I
> cut them at 4 microns and put them on charged slides. We are having a lot
> of issues with the receiving lab saying the tissue is falling off. Their
> supervisor says the sections are too thick (remember 4 microns) and my
> pathologist says they are not too thick. Any suggestions for resolving this
> issue with the receiving lab?
>
> Thanks!
> --
> Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio
> Kettering, Ohio 937.297.0028 ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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> message.
>
> --
>
> Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio
> Kettering, Ohio 937.297.0028
>
> CONFIDENTIALITY NOTICE: This e-mail message, including any attachments,
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> please
> contact the sender by reply e-mail and destroy all copies of this original
> message.
>
> --
>
> Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio
> Kettering, Ohio 937.297.0028
>
> CONFIDENTIALITY NOTICE: This e-mail message, including any attachments,
> is for the sole use of the intended recipient(s) and may contain
> confidential
> or legally privileged information. Any unauthorized review, use, disclosure
> or distribution is prohibited. If you are not the intended recipient,
> please
> contact the sender by reply e-mail and destroy all copies of this original
> message.
>
-- 
Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio
Kettering, Ohio 937.297.0028
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Re: [Histonet] IHC

[Morken, Timothy via Histonet]

Emily, ? Are you ensuring excess water is not under the section that would 
prevent adherence to the slide? Are you using slides recommended by the outside 
lab? Do they recommend a procedure for drying the slides before sending?
Just a starting point...

Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

-Original Message-
From: Emily Horst via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, August 10, 2017 2:42 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC

Hi everyone,

We send our IHCs to an outside lab. I send them the unstained slides. I cut 
them at 4 microns and put them on charged slides. We are having a lot of issues 
with the receiving lab saying the tissue is falling off. Their supervisor says 
the sections are too thick (remember 4 microns) and my pathologist says they 
are not too thick. Any suggestions for resolving this issue with the receiving 
lab?

Thanks!
--
Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio 
Kettering, Ohio 937.297.0028 ___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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[Histonet] IHC

[Emily Horst via Histonet]

Hi everyone,

We send our IHCs to an outside lab. I send them the unstained slides. I cut
them at 4 microns and put them on charged slides. We are having a lot of
issues with the receiving lab saying the tissue is falling off. Their
supervisor says the sections are too thick (remember 4 microns) and my
pathologist says they are not too thick. Any suggestions for resolving this
issue with the receiving lab?

Thanks!
-- 
Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio
Kettering, Ohio 937.297.0028
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[Histonet] Histotech Job near Northern NJ/NY State Line

[Melissa Owens via Histonet]

Hi Histonetters,

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contact me if you would like more details. This does require a New York 
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histonetters. We will be exhibiting at booth 632. Come by!

Melissa Owens
President, Laboratory Staffing

Direct Line: 407.413.9117

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[Histonet] SmartWrite cassette printer

[Rosalie N. Agrow via Histonet]

Looking for users with the Sakura SmartWrite Cassette Printer to compare notes. 
 Please contact me at your convenience.  Thank you in advance.

Rosalie Agrow, B.S., CT(ASCP) | Manager | Anatomic Pathology Laboratory |
1968 Peachtree Road | 77 Building, 4th Floor | Atlanta, GA 30309
O: 404-605-2142 |F: 404-609-6645 |E: 
rosalie.ag...@piedmont.org




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Re: [Histonet] "Dirty" Immunos

[Elizabeth Chlipala via Histonet]

Paula

You need to sit down with them and review the slides together, you can't solve 
a problem without seeing the problem.  They need to explain and show you what 
they consider dirty.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-Original Message-
From: Paula via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, August 10, 2017 8:24 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] "Dirty" Immunos

Hello,

Hoping we could get a recommendation on helping this issue..

We have a pathologist that complains about "dirty" stains every now and 
then..not all the time. What could this be/mean? Could it be debris, background 
staining?

We use the Bond III with their ready to use antibodies and their slides for 
tissue controls.  

 

Regarding the "dirty" complaint, could it come from our water baths, 
covertiles, paraffin, antibodies? 

 

I appreciate any feedback to help and I'll try anything.

Thank you,

Paula

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