[Histonet] Fwd: Asking for CV-5000 cover slipper sewrvice software.

[Carlos Torres Vega via Histonet]

Enviado desde mi iPhone

Inicio del mensaje reenviado:

> De: Carlos Torres Vega 
> Fecha: 12 de febrero de 2024, 11:39:55 a.m. GMT-6
> Para: histonet@lists.utsouthwestern.edu
> Asunto: Asking for CV-5000 cover slipper sewrvice software.
> 
> 
> Does anybody have the software for service a Leica CV-5000 cover slippers.
> I will appreciate a lot if any body can share it to me. 
> Thanks a lot
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[Histonet] Fwd: Hilda, Congrats on Making It Halfway!

[RENE MORALES via Histonet]

   Sent from my iPhone

   Begin forwarded message:

   From: Toyota Financial Services 
   Date: April 18, 2023 at 15:02:16 PDT
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   Subject: Hilda, Congrats on Making It Halfway!
   Reply-To: Toyota Financial Services
   

   

   Stay on the road to ownership.
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   Principal‐only payments are extra payments (in addition to your
   scheduled monthly installment payments) to pay down the remaining loan
   amount. These payments apply directly to your principal loan balance,
   help pay off your contract sooner and save money on interest.^2

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  10. 

[Histonet] Fwd: Ionis Pharmaceuticals has a fulltime position open in Carlsbad, Ca.:

[Jamie Watson via Histonet]

Hello Everyone,

We have a fulltime Research Associate/Senior Research Associate, Histology 
position

https://recruiting.ultipro.com/ISI1000ISIS/JobBoard/14a66400-68fa-4847-8593-af776328cfd2/OpportunityDetail?opportunityId=7ecb097d-bf27-4db4-8d4d-1b6676353338

Ionis is seeking a talented and highly motivated Histotechnician to expand our 
excellent team. Work in a state-of the art histology lab and join a highly 
collaborative team. Under general supervision of the Histology Associate 
Director, prepare microscopic tissue slides for research purposes. Histological 
procedures such as: tissue preparation, sectioning, histochemical standard and 
special staining, single/multiplex IHC and ISH staining. Antibody/ISH probe 
workups, digitalizing glass slides and Image Analysis. Quality control, trouble 
shooting, adding new ideas/technologies. A great environment to expand your 
histology knowledge and experience.

RESPONSIBILITIES:
·Preparing microscopic slides on human/animal tissue for 
research/diagnostic purposes, including processing, embedding, microtomy, and 
mounting to include both paraffin processed tissue and frozen sectioning
·Performing complex histochemical and immunohistochemical stains, data 
recording, and maintaining instruments
·Quality control and quality assurance procedures, performing routine 
and special staining
·Slide organization, preparing and maintaining all necessary reagents, 
including stains, alcohols, and paraffins according to proper specifications
·Handling and disposing of hazardous materials
·Processing specimen accessioning to include all manual and 
computerized data entry
·Performing other duties as assigned
QUALIFICATIONS:
·3-10 years of Histology laboratory experience
·Proficient with tissue processing, embedding, microtomy, histochemical 
stains and automated IHC equipment
·Strong interpersonal skills and ability to be successful in a team 
environment
·Computer data entry (confident/proficient with Word, Excel, SharePoint)
·Understanding and basic use of Image Analysis desired
·Proficiency in algebraic equations
·Willingness to work with hazardous chemicals
MINIMUM QUALIFICATIONS:
·Education and experience equivalent to: Associate Degree
·HT(ASCP) Histotechnician Certification desired

Jamie Watson
Associate Director, Histology

jwat...@ionisph.com
D: (760)603-2583855 Gazelle Court, Carlsbad, CA 92010



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Re: [Histonet] Fwd[2]: Re: AFIP Manual

[Tony Henwood (SCHN) via Histonet]

Hi Maxim,

Excellent
At least this amazing piece of history can live on.

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA


From: Пешков Максим via Histonet 
Sent: Saturday, 11 July 2020 03:23
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fwd[2]: Re:  AFIP Manual

Dear Bob!
Free download AFIP manuals:
4th ed:  
https://books.google.ru/books/about/Laboratory_methods_in_histotechnology.html?id=R1xrMAAJ_esc=y
3rd ed:  
https://archive.org/details/ManualHistologicalStaining/mode/2up?q=staining+methods+afip+manual
2nd ed:  https://archive.org/details/manualofhistolog00arme/page/n1/mode/2up  
или  
https://books.google.ru/books?id=CFRBYAAJ=frontcover=ru=gbs_ge_summary_r=0#v=onepage=false
1st ed:  
https://books.google.ru/books?id=vx1rMAAJ=frontcover=ru=gbs_ge_summary_r=0#v=onepage=false
Enjoy, please.
Maxim Peshkov,
Russia,
Taganrog.


--
Пешков Максим
--

--
Пешков Максим

--

--
Пешков Максим

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[Histonet] Fwd[2]: Re: AFIP Manual

[Пешков Максим via Histonet]

 
Dear Bob!
Free download AFIP manuals:
4th ed:  
https://books.google.ru/books/about/Laboratory_methods_in_histotechnology.html?id=R1xrMAAJ_esc=y
3rd ed:  
https://archive.org/details/ManualHistologicalStaining/mode/2up?q=staining+methods+afip+manual
2nd ed:  https://archive.org/details/manualofhistolog00arme/page/n1/mode/2up  
или  
https://books.google.ru/books?id=CFRBYAAJ=frontcover=ru=gbs_ge_summary_r=0#v=onepage=false
1st ed:  
https://books.google.ru/books?id=vx1rMAAJ=frontcover=ru=gbs_ge_summary_r=0#v=onepage=false
Enjoy, please.
Maxim Peshkov,
Russia,
Taganrog.
 
 
--
Пешков Максим  
--
 
--
Пешков Максим
   
--
 
--
Пешков Максим
 
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[Histonet] Fwd: Sectioning mice vertebrae and tail

[lucius lo via Histonet]

-- Forwarded message -
From: lucius lo 
Date: Fri, 21 Feb 2020 at 15:46
Subject: Sectioning mice vertebrae and tail
To: 


To whom may concern,

I am a University student who currently pursuing a field in histology
technician and is having a final year of the histology of bone marrow
adipocytes in transgene mice.

My question is regarding the sectioning of these fat-rich tissues which
torn off easily.

My way of sectioning is:
1. Freeze the blocks for an hour in wet ice immersed in distilled water.
2. Trim off the block in one micron while pressing down the lever.
3. Section the slide in five or six microns, then freeze the section again
after trimmed off a hundred microns.
4. Obtain another section after placing the block in ice for 30 seconds.

The torn off issue are highly corrected after I do step 4. However, *these
tissues still appear with tiny holes that only appear after the H*.
I would like to ask if anyone could share their experiences or solutions in
facing tails and vertebrae.

Best,
Lucius
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[Histonet] Fwd: Available Histology job: Houston, Texas

[Jackie Hardamon via Histonet]

Hi,

I’ve subscribed to the forum. Please reconsider my post for job listing. 

Jaclyn Hardamon, HT(ASCP)cm QIHC
Client Labs Histology Supervisor
jackie.harda...@mldpathology.com 

MLD Pathology
1140 Business Center Drive, Suite 370
Houston, Texas  77043
M: (832) 657-4356
P: (713) 271-6881
F: (713) 271-6885
www.mldpathology.com

Begin forwarded message:

> From: Jackie Hardamon 
> Date: February 4, 2020 at 8:56:39 PM CST
> To: histonet@lists.utsouthwestern.edu
> Subject: Available Histology job: Houston, Texas
> 
> Hi,
> 
> I’d like to post my company’s open positions on your forum. We are seeking 
> two Histology Techs to work at remote lab locations throughout Houston. 
> 
> https://docs.google.com/document/d/1RCNlRxY9knXseQB9i8mNCtaII5npNqwTRSEEByHPUcM
> 
> Applicants may contact me at the info provided below. 
> 
> Many thanks,
> 
> Jaclyn Hardamon, HT(ASCP)cm QIHC
> Client Labs Histology Supervisor
> jackie.harda...@mldpathology.com 
> 
> MLD Pathology
> 1140 Business Center Drive, Suite 370
> Houston, Texas  77043
> M: (832) 657-4356
> P: (713) 271-6881
> F: (713) 271-6885
> www.mldpathology.com
> 
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[Histonet] Fwd: Histology Benchmarks

[Ledee,Desiree Y via Histonet]

Get Outlook for iOS

From: Ledee,Desiree Y 
Sent: Tuesday, November 12, 2019 7:07:51 PM
To: naje1...@yahoo.com 
Subject: Re: [Histonet] Histology Benchmarks

The amounts are annually , not research and located in Omaha Nebraska. We have 
a consultant company Accumen indicating we only need 4.5 histo techs and 4 lab 
assistant to cover all areas Including maintenance etc. They are using 
productivity benchmarks written by Rene Buesa in 2010



Get Outlook for iOS

From: cynthia haynes 
Sent: Tuesday, November 12, 2019 6:42:50 PM
To: Ledee,Desiree Y 
Subject: Re: [Histonet] Histology Benchmarks

CAUTION: This email is not from a CHI source. Only click links or open 
attachments you know are safe. Please send as an attachment all spam/phishing 
and unusual emails to s...@catholichealth.net.

The amounts you gave, is this annual or research that needs to be done. If the 
amounts are annually you will probably need 10-25 Technologist . Where are you 
located?

Sent from Yahoo Mail on 
Android

On Tue, Nov 12, 2019 at 6:26 PM, Ledee,Desiree Y via Histonet
 wrote:
How many techs do I need:
135333 blocks
40,000 IHC stains/ISH/predictive markers
23,000 Recuts/unstains
H 145346
Supports 8 hospital sites - slides sent out to pathologist at sites.


Get Outlook for 
iOS>



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Re: [Histonet] Fwd: [HISTONET] Biocare

[Rathborne, Toni via Histonet]

I got this reply from my Biocare rep.

We are not going out of business   What happened is over a year ago we were 
acquired by a Venture group, which is a good thing. They infuse money and are 
making us stronger.





NOTICE: This e-mail and its attachments, if any, may contain legally privileged 
and/or confidential information protected by law. It is intended only for use 
by the named addressee(s). If you are not the intended recipient of this 
e-mail, any dissemination, distribution or copying of this e-mail and its 
attachments, if any, is strictly prohibited. If you have received this 
transmission in error, please immediately notify the sender by telephone or by 
reply e-mail, and permanently delete this e-mail and the attachments, if any, 
and destroy any printouts.

-Original Message-
From: Colleen Forster via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, September 12, 2019 1:37 PM
To: Maria Cruz
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Fwd: [HISTONET] Biocare


*** This is an External Email ***

I'd love to know who actually told Michael this? I've had a company try
this on me as wellinterested to know if it is the same company.

C. Forster

On Thu, Sep 12, 2019 at 12:27 PM Maria Cruz via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Mr. Bradley:
> This is how awful rumors get started.  I’m sure that Biocare is not going
> out of business.  They’ve recently released a new stainer and last weak an
> IVD-labeled antibody for P16.  If a sales rep from another company told you
> this they are pretty unethical and could learn a few things from the people
> at Biocare.
> Maria
>
> -- Forwarded message --
> From: Michael Bradley 
> To: Histopeeps Histonet 
> Cc:
> Bcc:
> Date: Tue, 10 Sep 2019 00:28:08 +0200
> Subject: [Histonet] Biocare
>
> Hi all
>
> I heard a rumor that Biocare is going out of business. Can anyone confirm
> or deny this?
>
> Thanks.
> Sent using the mail.com
>  mail app
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet=DwIGaQ=LfJFs5tz11XIvZ1zGnYRWYcpprcdQWHKbyr0OjT-Gjk=OywojvDeqnDOvbIWXIx1jW-8xZXD1RJBnKKp8Mh6i_g=qNyceGRYNM7Hsa4aC7FIQrlWODVZ8apJdZ59lI77YzM=PdsiEeE0Poe3SZ4E5Uszual_S5eiuQL0MNvYUG_fdP4=
>


--
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
B173 PWB  612-626-1930
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Re: [Histonet] Fwd: [HISTONET] Biocare

[Colleen Forster via Histonet]

I'd love to know who actually told Michael this? I've had a company try
this on me as wellinterested to know if it is the same company.

C. Forster

On Thu, Sep 12, 2019 at 12:27 PM Maria Cruz via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Mr. Bradley:
> This is how awful rumors get started.  I’m sure that Biocare is not going
> out of business.  They’ve recently released a new stainer and last weak an
> IVD-labeled antibody for P16.  If a sales rep from another company told you
> this they are pretty unethical and could learn a few things from the people
> at Biocare.
> Maria
>
> -- Forwarded message --
> From: Michael Bradley 
> To: Histopeeps Histonet 
> Cc:
> Bcc:
> Date: Tue, 10 Sep 2019 00:28:08 +0200
> Subject: [Histonet] Biocare
>
> Hi all
>
> I heard a rumor that Biocare is going out of business. Can anyone confirm
> or deny this?
>
> Thanks.
> Sent using the mail.com
>  mail app
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


-- 
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
B173 PWB  612-626-1930
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[Histonet] Fwd: [HISTONET] Biocare

[Maria Cruz via Histonet]

Mr. Bradley:
This is how awful rumors get started.  I’m sure that Biocare is not going
out of business.  They’ve recently released a new stainer and last weak an
IVD-labeled antibody for P16.  If a sales rep from another company told you
this they are pretty unethical and could learn a few things from the people
at Biocare.
Maria

-- Forwarded message --
From: Michael Bradley 
To: Histopeeps Histonet 
Cc:
Bcc:
Date: Tue, 10 Sep 2019 00:28:08 +0200
Subject: [Histonet] Biocare

Hi all

I heard a rumor that Biocare is going out of business. Can anyone confirm
or deny this?

Thanks.
Sent using the mail.com
 mail app
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[Histonet] Fwd: VIP 5

[Patti Nelson via Histonet]

Patti Nelson HT (ASCP)   909-841-9761   
 Sent from my iPhone

Begin forwarded message:

> From: Patricia Nelson 
> Date: February 7, 2019 at 8:38:23 AM PST
> To: "nelsonr...@verizon.net" 
> Subject: FW: VIP 5
> 
>  
> From: Patricia Nelson
> Sent: Thursday, February 07, 2019 8:37 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: VIP 5
> 
> Hi Histo World,
>  
> HELP!!Mid December our VIP 5 went down. I work in a GI lab with a 
> workload of 300 daily. Our lab obtained another refurbished VIP 5. But now we 
> are starting to see the same symptoms as we seen on our old VIP before it 
> went down. Some of the symptoms are formalin carry over (formalin level goes 
> down after each run), our 95 w/eosin is carrying over to all solutions all 
> the way to the paraffin. Now our small GI bx are starting to feel unfixed and 
> has patchy staining. Has anyone dealt with any of these issues? 
> We maintenance our VIP weekly and change all solutions and all paraffins Once 
> a month we do our warm water flush. Below is our set up
>  
> 1st level: Formalin, Formalin, 70%, 80%, 95% w/eosin,
> 2nd level: 100,100, 100, xylene, xylene
> 3rd level: cleaning xylene, cleaning alcohol, water, empty, fume con. water
>  
> Patti Nelson (HT ASCP)
> United Gastroenterologists
>  
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[Histonet] Fwd: Used slide disposal

[Charles Riley via Histonet]

Do stained slides have to be disposed of as hazardous material or can they
be thrown in the regular trash if properly packaged (via hard plastic
sharps container or broken glass box)?



-- 

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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[Histonet] Fwd: Specimen's from hospital to lab.

[Gareth Davis via Histonet]

I sent this question out last week, someone answered and I guess I
accidentally deleted the email.  So, if anyone remembers answering my
question about private labs doing hospital outpatient biopsies, please
email me again.
Thanks,
Gareth Davis
-- Forwarded message --
From: Gareth Davis 
Date: Mon, Nov 20, 2017 at 9:36 AM
Subject: Specimen's from hospital to lab.
To: "Histonet@lists.utsouthwestern.edu" 


Are there any labs that are private, but receive specimens from a
hospital?  If so, what kind of protocol or policy do you give the hospital
to follow in order for them to submit specimens to the lab?
Thanks,

-- 
*Ms. Gareth B. Davis*, B.S., HT, QIHC (ASCP)cm
Yuma Gastroenterology
Yuma, AZ 85364
928-248-5259 <(928)%20248-5259>




-- 
*Ms. Gareth B. Davis*, B.S., HT, QIHC (ASCP)cm
Yuma Gastroenterology
Yuma, AZ 85364
928-248-5259
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[Histonet] Fwd: Tissue-Tek VIP 6 AI Vacuum Infiltration Processor

[Montse Verdú via Histonet]

Dear histonetters,



Does anyone experience with the Tissue-Tek VIP 6 AI vacuum infiltration
procesor?

How often do you experience problems with these instruments that you need
to

have service for them? What kind of problems are they?



Thank you in advance!



Montse Verdú

Histopat laboratoris

Tel +34932033000 <+34%20932%2003%2030%2000>

Biopat. Biopatologia Molecular

Tel+34932030837 <+34%20932%2003%2008%2037>

mve...@histopat.es



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distribució, divulgació o reproducció de aquesta comunicació per part de
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aquest missatge per error, si-us-plau, contacti amb la persona que figura
com a remitent i procedeixi a la seva eliminació.



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distribución, divulgación o reproducción de esta comunicación por parte de
personas o entidades distintas al destinatario está prohibida. Si ha
recibido este mensaje por error, por favor, contacte con la persona que
figura como remitente y proceda a su eliminación.



This message and its attachments are addressed exclusively to the person or
entity indicated as its named addressee, and may contain data and/or
confidential information, subject to professional secret or whose
disclosure is prohibited by virtue of current legislation in force.  Any
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[Histonet] Fwd: coverslipper Dako question

[Montse Verdú via Histonet]

Dear histonetters,



Does anyone experience with the Coverslipper Dako?



How often do you experience problems with these instruments that you need
to

have service for them? What kind of problems are they?



In our case the drawer is not securely attached, the magnet is not securely
attached to the equipment so the basket collides with the drawer and breaks
the slides.

Does anyone else have this same problem?



Thank you in advance!



Montse Verdú

Histopat laboratoris

Tel +34932033000 <+34%20932%2003%2030%2000>

Biopat. Biopatologia Molecular

Tel+34932030837 <+34%20932%2003%2008%2037>

mve...@histopat.es



Aquest missatge i els seus annexos estan adreçats exclusivament a la
persona o entitat que figura com a destinatari i poden contenir dades i/o
informació confidencial, sotmeses a secret professional o la divulgació de
les quals estigui prohibida en virtut de la legislació vigent. Tota
distribució, divulgació o reproducció de aquesta comunicació per part de
persones o entitats diferents del destinatari està prohibida. Si ha rebut
aquest missatge per error, si-us-plau, contacti amb la persona que figura
com a remitent i procedeixi a la seva eliminació.



Este mensaje y sus anexos van dirigidos exclusivamente a la persona o
entidad que figura como destinatario, y pueden contener datos y/o
información confidencial, sometidos a secreto profesional o cuya
divulgación esté prohibida en virtud de la legislación vigente. Toda
distribución, divulgación o reproducción de esta comunicación por parte de
personas o entidades distintas al destinatario está prohibida. Si ha
recibido este mensaje por error, por favor, contacte con la persona que
figura como remitente y proceda a su eliminación.



This message and its attachments are addressed exclusively to the person or
entity indicated as its named addressee, and may contain data and/or
confidential information, subject to professional secret or whose
disclosure is prohibited by virtue of current legislation in force.  Any
distribution, disclosure or reproduction of this type by persons or
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received this message by error, please contact the person indicated as its
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[Histonet] Fwd: Dissolving precipitate in formalin?

[Conway, Carla via Histonet]

Hi Lauren,

Thanks so much for your reply. I've seen the precipitate you refer to, but
this looks really different. Many of the flakes are large (5-7 mm) and were
at the bottom of the bottle. The bottles were previously unopened and
I didn't notice the flakes until preparing fixative for a study this
morning. Am wondering if adding NaOH will help (similar to dissolving
paraformaldehyde for EM fixative).

Thanks again!

Carla


Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 USA
Phone: 206-526-2042
Fax: 206-526-6654
E-mail: cmcon...@usgs.gov

-- Forwarded message --
From: Lauren Sweeney 
Date: Tue, Aug 22, 2017 at 8:02 AM
Subject: RE: [Histonet] Dissolving precipitate in formalin?
To: "Conway, Carla" 


I've always been told that that is the salts flaking out and that you can't
dissolve them, usually found accumulating around the spigot or opening of
wherever it is poured out, we always just brush it off and if re-using the
container, can dissolve after running under very hot tap water. Hope this
helps!

-Original Message-
From: Conway, Carla via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, August 22, 2017 10:39 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Dissolving precipitate in formalin?

Hello,

I have 4 bottles of formalin which contain white flakes of paraformaldehyde
precipitate. I tried to dissolve the flakes by heating to ~65 degrees C for
45 min (with stirring). Has anyone been successful in dissolving this type
of precipitate?

Thanks very much,

Carla

Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 USA
Phone: 206-526-2042
Fax: 206-526-6654
E-mail: cmcon...@usgs.gov
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[Histonet] Fwd: RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017

[Eileen Akemi Allison via Histonet]

Pam: No one in their right mind would work in that lab in Modesto!  They have a 
horrible track record for numerous years! Run and don’t even think about 
applying! 
Akemi

> Begin forwarded message:
> 
> From: "Pam Barker" 
> Subject: RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017
> Date: August 1, 2017 at 1:26:51 PM PDT
> To: "Akemi Allison" 
> 
> Hi Akemi!
> What are you doing on your summer vacation?  
> Been anyplace you would like to move to?   
> Whatever you want to do, wherever you want to be, I can help!
>  
> There is still time this summer to make that move.  
> I have opportunities in all kinds of labs in all areas of the country. 
> Your next position is just a phone call or an e-mail away.
>  
> All of the opportunities that I represent are permanent full time positions 
> with companies that offer excellent salaries, benefits and relocation 
> assistance.  
>  
> Here are some of the opportunities that I am most excited about:
>  
> HISTOLOGY SUPERVISORS AND MANAGERS:
> Histology Lab Manager – Atlanta, GA
> Histology Supervisor – Atlanta, GA
> Histology Supervisor – Boston, MA
> Histology Supervisor – Irvine, CA
> Histology Supervisor – Modesto, CA
> Lead Histotech – Los Angeles – BRAND NEW LAB
> Study Director – Fort Collins CO
> HISTOTECHNICIANS/HISTOTECHNOLOGISTS
> CA – San Diego – brand new lab CLIA qualified to gross
> CA – Irvine – learn IHC
> CA – Los Angeles – Lead Histotech Brand New Lab!
> TX – Nacogdoches day shift hospital environment
> CO – Glenwood day shift hospital environment
> TN – Chattanooga – CLIA qualified to gross 3rd shift generous shift diff.
> MA – Greater Boston Area  - 2nd and 3rd shift generous shift diff.
>  
> If you happen to know anyone I am also looking for a cytotech in Clearwater, 
> FL
>  
> If you or anyone you know are interested in hearing more about any of these 
> opportunities or have another type of position or area in mind and would like 
> some help in your job search please let me know.  Just shoot me an e-mail at 
> rel...@earthlink.net  or give me a call toll 
> free at 866-607-3542 or on my cell at 407-353-5070.  
>  
> Thanks-Pam
>  
> Right Place, Right Time, Right Move with RELIA!
>  
> Thank You!
>  Pam M. Barker
>  
> Pam Barker
> President/Senior Recruiting Specialist-Histology
> RELIA Solutions
> Specialists in Allied Healthcare Recruiting
> 5703 Red Bug Lake Road #330
> Winter Springs, FL 32708-4969
> Phone: (407)657-2027
> Cell: (407)353-5070
> FAX: (407)678-2788
> E-mail: rel...@earthlink.net 
> https://www.facebook.com/RELIASolutionsforhistologyprofessionals 
> 
> www.facebook.com /PamBarkerRELIA
> www.linkedin.com/in/reliasolutions 
> www.twitter.com/pamatrelia 
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[Histonet] Fwd: A suitable negative control for BAP1

[Patrick Laurie via Histonet]

Hello everyone,

We recently brought BAP1 into our IHC menu, and we were eventually able to
get a good stain that our pathologist was happy with.  We are, however,
looking for a good suitable negative control.  Our pathologist is not
certain and wanted me to ask the local community.

Thanks,

Patrick Laurie(HT)ASCP QIHC

Histology Manager

Celligent Diagnostics, LLC

101 East W.T. Harris Blvd  | Suite 1212 | Charlotte, NC 28262

Work: 704-970-3300  Cell: 704-266-0869
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Re: [Histonet] Fwd:

[Rene J Buesa via Histonet]

As I see it, the best solution is "1"Even more: if the piece of tissue is large 
enough,→cut 1 section and stain → select at least 2 (+) areas→ divide the block 
into 2 blocks each containing one of those 2 areas and by doing so you would 
have duplicated the number of possible (+) sections.René 

On Monday, May 2, 2016 3:09 PM, Linda Margraf via Histonet 
 wrote:
 

 

> From: Cindy Bulmer 
> Date: May 2, 2016 at 12:15:22 PM CDT
> To: histonet-ow...@lists.utsouthwestern.edu
> 
> Hello Histoland,
>  
> I need some advice, I have a PT block that is positive with Spirochetes.
> What would be the best way to use this block as a positive control?
>  
> 1)  Cut (serial sections, stain the last slide for bugs) and oven time (60) 
> for 1 hr.
>      then put slides in refrigerator for future use.
> 2)  Cut (serial sections, stain the last slide for bugs) NO oven time  and 
> put slides
>      directly in refrigerator for future use.
> 3)  Cut "fresh" every time they order the Ab.
>  
> Thank you,
> Cindy
> Cynthia Bulmer HT(ASCP),QIHC
> IHC Supervisor, CTPL
> Waco, TX
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[Histonet] Fwd:

[Linda Margraf via Histonet]

> From: Cindy Bulmer 
> Date: May 2, 2016 at 12:15:22 PM CDT
> To: histonet-ow...@lists.utsouthwestern.edu
> 
> Hello Histoland,
>  
> I need some advice, I have a PT block that is positive with Spirochetes.
> What would be the best way to use this block as a positive control?
>  
> 1)  Cut (serial sections, stain the last slide for bugs) and oven time (60) 
> for 1 hr.
>  then put slides in refrigerator for future use.
> 2)  Cut (serial sections, stain the last slide for bugs) NO oven time  and 
> put slides
>  directly in refrigerator for future use.
> 3)  Cut "fresh" every time they order the Ab.
>  
> Thank you,
> Cindy
> Cynthia Bulmer HT(ASCP),QIHC
> IHC Supervisor, CTPL
> Waco, TX
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[Histonet] Fwd: Natalia's Credentials

[Charles Riley via Histonet]

My lab is looking to hire a tech who has been trained in another country.
Management wants to know if their credentials qualify them to work in a CAP
accredited lab without direct supervision  or if they will need to sit for
the ASCP board exam. If they need to sit for the exam do her credentials
qualify her to take the exam now? Her credentials are listed below

Ministry of Public Health & Social Care Diploma

Graduated the full course of education at Institute of Health Care
Employees with Secondary Professional Education Bulgarian Medical Academy.

Qualification of Clinical Laboratory Assistant

Transcript includes:
Laboratory Equipment and Laboratory Appliances
Clinical Laboratory
Histology with General Pathology & Histological Technique
Biochemistry

120 hours of Histological Laboratory

-- 

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs

Doctors Pathology Services, Dover DE
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Re: [Histonet] Fwd: Natalia's Credentials

[Morken, Timothy via Histonet]

Charles, Foreign course work is useless for CLIA until it is vetted by a 
transcript service that can evaluate the validity of the school and how that 
course work matches with the required typical US courses. ASCP requires such 
vetting for foreign applications for ASCP certification (I've been involved 
with a few people taking that route). It can take weeks to months to get such 
verification depending on the bureaucracy involved in the foreign country. And 
the applicant has to pay for it.

Experience counts, but you don't know what the quality of that is either until 
you see her in action.

If this person is already in the US and local to you, and you still want to 
pursue it,  I suggest hiring her as a lab assistant with the idea you would 
evaluate her skills on site, if you are willing to do that.



Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center


-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, February 12, 2016 7:19 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fwd: Natalia's Credentials

My lab is looking to hire a tech who has been trained in another country.
Management wants to know if their credentials qualify them to work in a CAP 
accredited lab without direct supervision  or if they will need to sit for the 
ASCP board exam. If they need to sit for the exam do her credentials qualify 
her to take the exam now? Her credentials are listed below

Ministry of Public Health & Social Care Diploma

Graduated the full course of education at Institute of Health Care Employees 
with Secondary Professional Education Bulgarian Medical Academy.

Qualification of Clinical Laboratory Assistant

Transcript includes:
Laboratory Equipment and Laboratory Appliances Clinical Laboratory Histology 
with General Pathology & Histological Technique Biochemistry

120 hours of Histological Laboratory

-- 

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs

Doctors Pathology Services, Dover DE
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[Histonet] Fwd: Acetone and IHC

[Linda Margraf via Histonet]

Sent from my iPhone

Begin forwarded message:

> From: "Dyer, Terry" 
> Date: January 18, 2016 at 11:59:14 AM CST
> To: "'histonet-ow...@lists.utsouthwestern.edu'" 
> 
> Subject: Acetone and IHC
> 
> I would appreciate input in reference to cytology button preparations and IHC 
> staining.
> Our laboratory commonly uses acetone and alcohol to make cytology buttons 
> from body fluids.  We often perform IHC on these
> Cell blocks.  Does anyone use acetone for cytology preparation?  If so 
> does this cause staining issues or hinder staining of the
> Cell block?
> Thank you,
> Terry Dyer HT (ASCP)
>Torrance Memorial Medical Center
>3330 Lomita Blvd.
>Torrance, CA.  90505
> 310-325-9110 X1740
>  
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[Histonet] Fwd: instrument manuals

[yesyes]

- Forwarded Message -

From: yes...@comcast.net 
To: histonet@lists.utsouthwestern.edu 
Sent: Monday, May 18, 2015 7:22:59 PM 
Subject: instrument manuals 

Hi, I'm doing some labkeeping and fould some old instrument manuals.  If anyone 
is interested in them, let me know and I can send them to you. 
  
Shandon Embedding Center Model 6404  - old model 
Leica RM 2125  Rotary microtome 
Hyperclean Fume extraction hood 
Tissue Tek VIP 5 operating manual 
Leica Autostainer XL Reference Manual 
Leica Autostainer XL Instruction Manual 
Protocol MicroProbe staining system 
Tissue Tek TEC 5 Tissue embedder 
  
  
  

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[Histonet] Fwd: mouse tibia histology

[Shruti Shah]

Sent from my iPhone

Begin forwarded message:

From: Shruti Shah s.s...@garvan.org.aumailto:s.s...@garvan.org.au
Date: 21 April 2015 7:28:07 pm AEST
To: Garrey Faller garr...@gmail.commailto:garr...@gmail.com
Cc: 
histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] (no subject)

Hi does any one doing mice tibia histology, we use to fix in formalin for 24 
hours and transfer in 2and half weeks in 0.5M EDTA three change and process for 
24 hours long protocol in automatic processor.
But I am facing problem with bone marrow shrinkage. If any one have idea for 
decalcification timing and solution can resolved this problem and keep bone 
marrow intact with bone.
Thank you in advance.

Regards,
Shruti

Sent from my iPhone

On 21 Apr 2015, at 8:51 am, Garrey Faller 
garr...@gmail.commailto:garr...@gmail.com wrote:

Here is the CAP checklist requirement:
ANP.21450
All  histochemical stains are of adequate quality, and daily controls are
demonstrated on each day of use for the tissue components or organism for
which they were designed.

Ray...you should call the CAP and ask for guidance on this.
My interpretation of this requirement is that it should be OK to use a
fungus from an orange peel. An orange peel fungus should have the same
staining characteristics as a candida or aspergillus etc.  Similarly a
bacteria is a bacteria. If you can produce a control that has both gram
positives and negatives, it should be OK. But, don't quote me on this.

Call the CAP for a definitive answer. I am interested in their response.
Garrey

On Sun, Apr 19, 2015 at 9:06 PM, 
koelli...@comcast.netmailto:koelli...@comcast.net wrote:

I asked about this in a different vein months ago.  Has anyone shown a
strawberry or ground meat or slim jim or orange peel as a bacteria/fungus
control used for diagnostics to an inspector inspecting the lab and was
there any comment from the inspector either positive or negative. Never
heard back anything.
Ray, Lake Forest Park, WA

- Original Message -

From: tjfinney2...@gmail.commailto:tjfinney2...@gmail.com
To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu
Sent: Sunday, April 19, 2015 5:24:53 PM
Subject: [Histonet] (no subject)

GMS controls
From my understanding we can't use non human controls on patients. I
could be wrong, but you may want to look into it.

Happy Connecting.  Sent from my Sprint Phone.

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communication. If you have received this message in error please notify us at 
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[Histonet] Fwd: FW: IQMH Symposium, Toronto, ON CANADA June 4/5, 2015

[June Shin]

 *From:* June Shin
*Sent:* April-21-15 11:53 AM
*To:* 'histonet@lists.utsouthwestern.edu'
*Subject:* IQMH Symposium, Toronto, ON CANADA June 4/5, 2015



*The 2015 IQMH Symposium*

The demands of personalized medicine are upon us, and it is critical that
patient tissue specimens are confidently handled with the utmost of care.
In pathology, part of protecting patient safety involves having a reliable
mechanism to control and track patient tissue specimens. Exact knowledge of
ischemia time, fixation and processing of positively-identified patient
specimens are vital in identifying whether a human tissue specimen can be
utilized for subsequent biomarkers, molecular/genetic studies. This
symposium is of direct interest to those professionally engaged in
healthcare and medical research, and who are concerned with the handling of
human tissue; in particular, pathology laboratories, tissue banks and
research units. There will be interactive and plenary sessions, and an
opportunity for networking and industry exhibits.

*Theme:*

Best practices in pathology: knowing exactly how you handle each human
tissue specimen you process

*Date:*

June 4-5, 2015

*Venue:*

Hilton Garden Inn Toronto Airport
3311 Caroga Drive
Mississauga, ON L4V 1A3


* Registration*

*Registration is online only.*
Fee: $350 + HST (no refunds or cancellations)

*Register for this event:*
https://iqmh.org/Shop/Product-Viewer/slug/IQMH-Symposium-2015




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AW: [Histonet] Fwd: SOP on embedding tissues of different sizes in same mold

[Gudrun Lang]

We avoid to put specimens of different size into one cassette in general
(especially with biopsies). If I have too pieces of different size with a
clear cut side, I put them both on the ground of the mold. I think one could
never precisly put a specimen in a certain level into the mold, if it
doesn't lie on the bottom.
Gudrun

-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Sanjeet
Gesendet: Donnerstag, 31. Juli 2014 03:44
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Fwd: SOP on embedding tissues of different sizes in same
mold



Sent from my iPhone

Begin forwarded message:

 From: Sanjeet asanj...@yahoo.com
 Date: July 30, 2014 at 9:15:25 PM EDT
 To: histonet-requ...@lists.utsouthwestern.edu
histonet-requ...@lists.utsouthwestern.edu
 Subject: SOP on embedding tissues of different sizes in same mold
 
 Hi
 Does anyone in the Histo world have an SOP on how to embed tissues of
different sizes in the same mold. Do you embed in different levels depending
upon the size, the larger embedded first and the smallest at the end. Or do
you embed all in the same plane regardless of the tissue size. Are you able
to justify and salvage all tissue on the slide.
 Thank you all in advance
 Sanjeet 
 
 
 Sent from my iPhone
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AW: [Histonet] Fwd: Embedding skin

[Gudrun Lang]

As a first information, we use sliding microtomes with the knife set in an
angel (called declination).
We embed straight, because areas of same concistancy should be positioned in
one line and in the cutting direction.
Areas of more rigidity compress the smoother ones, if they are shifted in an
angle. 

If you have problems with the orientation of the tissue in the block, just
turn the blockholder.
Gudrun



-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Sanjeet
Gesendet: Donnerstag, 31. Juli 2014 03:46
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Fwd: Embedding skin



Sent from my iPhone

Begin forwarded message:

 From: Sanjeet asanj...@yahoo.com
 Date: July 30, 2014 at 9:27:46 PM EDT
 To: histonet-requ...@lists.utsouthwestern.edu
histonet-requ...@lists.utsouthwestern.edu
 Subject: Embedding skin
 
 Hi Histo techs
 
 Need some info on the correct orientation of skin tissue. Punch/ ellipse.
Does anyone have a literature on this topic. I am used to embed large skin
in an angle, the skin being on top. 
 Currently the place where I work have different protocol, the skin is
embedded straight, the epidermis being right angled to the mold.
 I find the section difficult to cut when the skin is embedded straight ,
the section are compressed and more chances scoring along the section.
 
 Thanks 
 Sanjeet
 
 Sent from my iPhone
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[Histonet] Fwd: Embedding skin

[Sanjeet]

Sent from my iPhone

Begin forwarded message:

 From: Sanjeet asanj...@yahoo.com
 Date: July 30, 2014 at 9:27:46 PM EDT
 To: histonet-requ...@lists.utsouthwestern.edu 
 histonet-requ...@lists.utsouthwestern.edu
 Subject: Embedding skin
 
 Hi Histo techs
 
 Need some info on the correct orientation of skin tissue. Punch/ ellipse. 
 Does anyone have a literature on this topic. I am used to embed large skin in 
 an angle, the skin being on top. 
 Currently the place where I work have different protocol, the skin is  
 embedded straight, the epidermis being right angled to the mold.
 I find the section difficult to cut when the skin is embedded straight , the 
 section are compressed and more chances scoring along the section.
 
 Thanks 
 Sanjeet
 
 Sent from my iPhone
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[Histonet] Fwd: SOP on embedding tissues of different sizes in same mold

[Sanjeet]

Sent from my iPhone

Begin forwarded message:

 From: Sanjeet asanj...@yahoo.com
 Date: July 30, 2014 at 9:15:25 PM EDT
 To: histonet-requ...@lists.utsouthwestern.edu 
 histonet-requ...@lists.utsouthwestern.edu
 Subject: SOP on embedding tissues of different sizes in same mold
 
 Hi
 Does anyone in the Histo world have an SOP on how to embed tissues of 
 different sizes in the same mold. Do you embed in different levels depending 
 upon the size, the larger embedded first and the smallest at the end. Or do 
 you embed all in the same plane regardless of the tissue size. Are you able 
 to justify and salvage all tissue on the slide.
 Thank you all in advance
 Sanjeet 
 
 
 Sent from my iPhone
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[Histonet] Fwd: [4]

[histopatty]

Hey! 
http://labidiomasaiac.usb.ve/-hit.of.sales?quhuniq=7590994ivyvyzj=922180




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[Histonet] [Fwd: EBV EBNA3A antibody (ab16126, Abcam)]

[avistarop]

 Mensaje original 
Asunto: EBV EBNA3A antibody (ab16126, Abcam)
De: avista...@ffyb.uba.ar
Fecha:  Mar, 21 de Enero de 2014, 5:45 pm
Para:
Cc: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
--


Hello to everyone!!!

Has anyone used this antibody ?

Sheep polyclonal to EBV EBNA3A antibody (ab16126, Abcam)

This antibody is used on FFPE tissue.

Could I please get a copy of your protocols?

Thank you so much

Aldana Vistarop



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[Histonet] Fwd: Histonet Digest, Vol 116, Issue 21

[Trevor Wicks]

twi...@sidra.org

Sent from Samsung Mobile

 Original message 
Subject: Histonet Digest, Vol 116, Issue 21
From: histonet-requ...@lists.utsouthwestern.edu
To: histonet@lists.utsouthwestern.edu
CC: 

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
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When replying, please edit your Subject line so it is more specific
than Re: Contents of Histonet digest...


Today's Topics:

   1. Transcriptionist Productivity (Willis, Donna G.)
   2. RE: CLIA Compliance Regulations for histology staff   coverage
  (Thurby, Christina)
   3. RE: Histonet Digest, Vol 116, Issue 20 (Scott A. Ely)


--

Message: 1
Date: Fri, 19 Jul 2013 17:09:57 +
From: Willis, Donna G. donna.wil...@baylorhealth.edu
Subject: [Histonet] Transcriptionist Productivity
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID:
2572b4d63b62e64a8078d8bbe34d4078312...@bhdasvexml2.bhcs.pvt
Content-Type: text/plain; charset=ISO-8859-1

For Managers of Anatomic Pathology Transcriptionist, do you have productivity 
standards for your staff?  If yes, would you please share them.

Thanks,

Donna Willis, HT/HTL (ASCP)
Anatomic Pathology Manager
Baylor University Medical Center-Dallas
ph. 214-820-2465 office
ph. 214-725-6184 mobile
donna.wil...@baylorhealth.edu

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are prohibited without proper authorization. If you are not the intended 
recipient (or have received this e-mail in error) please notify the sender 
immediately and destroy this e-mail. Any unauthorized copying, disclosure or 
distribution of the material in this e-mail is strictly forbidden and possibly 
a violation of federal or state law and regulations. Baylor Health Care System, 
its subsidiaries, and affiliates hereby claim all applicable privileges related 
to this information.


--

Message: 2
Date: Fri, 19 Jul 2013 13:52:59 -0400
From: Thurby, Christina christina.thu...@bms.com
Subject: [Histonet] RE: CLIA Compliance Regulations for histology
staff   coverage
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID:

e9c77491626ebf41bd449ea1098b040608a7d20...@ushpwbmsmmp007.one.ads.bms.com

Content-Type: text/plain; charset=us-ascii

Hi Akemi,
Please refer to this article in Advance magazine from 2010.  The second page 
has the CLIA regulations you are looking for.

Features:
The Grossing Histotechnologist in Surgical Pathology
Part 1 of this 2-part article explores regulatory requirements.
By Izak B. Dimenstein, MD, PhD, HT(ASCP)

Posted on: May 6, 2010

http://laboratory-manager.advanceweb.com/features/article-2/the-grossing-histotechnologist-in-surgical-pathology.aspx


Christina Thurby
Bristol Myers Squibb
812-307-2093



   5. Re: CLIA Compliance Regulations for histology staff  coverage
  (Jennifer MacDonald)
From:   Akemi Allison akemiat3...@yahoo.com
To: Histonet Histonet@lists.utsouthwestern.edu
Date:   07/18/2013 08:34 AM
Subject:[Histonet] CLIA Compliance Regulations for histology
staff
coverage
Sent by:histonet-boun...@lists.utsouthwestern.edu



Good morning to all in Histoland!

Hopefully, one of you can help me with a question which was proposed to
me
by a fellow histologist working in a very small GI histology lab.

Here is what information she gave me:  She is training a histology
assistant in house to do all the histology technical responsibilities at
the request of the GI doctors.  She said her OJT assistant is doing a
great job technically, but she has not yet registered in school to
finish
her  AA Degree so she can sit for the HT exam.  She has been working
under
her instruction for 1 year.  Her lab is California State and CLIA
licensed.

The GI doctors want the assistant to cover for her while she is on
vacation.  The pathologist who is the medical director does  not think
that CLIA Regulations allows this and wants the specimens sent out
during
her absence.  She needs the CLIA Regulations stating what the guidelines
are so her lab is complying to regulations.  She didn???t want to call
CLIA
because it would send a RED FLAG up.  I also didn???t want to call them
because they may ask me what the name of the lab was that was proposing
this question.

I told her that 

[Histonet] [FWD: Opportunity in Charleston, SC]

[Dingersoll]

   dingers...@aplaboratories.com


= ;

   




   




   


 = 


    Original Message 
   Subject: = Opportunity in Charleston, SC
   From: [1]dingers...@aplaboratories.com
   Date: Tue, March = 05, 2013 4:20 pm
   To: [2]histonet-requ...@lists.utsouthwestern.edu
   

   AP Laboratories, LLC, a rapidly= growing anatomic pathology laboratory
   in  Charleston, SC, is accepting appl= ications for full and part time
   histology  technicians/technologists  and  gro= ssing technicians.  We
   offer  an  excellent  compensation  and benefit pac= kage to full time
   employees. Pleaseemail   your   resume   to   [3]dingersoll@a   
plaboratories.com or visit our website at
   [4]www.aplaboratories.com   = nbsp;
   

   Donna S. Ingersoll, B.S., HTL, CT(ASCP)
   = 

   Laboratory Manager
   

   A P Laboratories LLC
   

   8= 43-300-3001 x 202
   

   [5]dingers...@aplaboratories.com
   


References

   1. 3Dmailto:Dingersoll@apl   2. 3Dmailto:histonet-request@lists.utsouthwes 
  3. file://localhost/tmp/3Dm   4. 3Dhttp://www.aplabor=/
   5. 
3Dmailto:dingersoll@aplaboratories.___
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[Histonet] Fwd: MIXED fiber type identification by Immunohistochemistry

[arjun ruhella]

Dear all,

Its been a while i asked for a doubt to all histology experts around US, but i 
have not been able to get much reply (only 1 :(). I thought my doubt/e mail 
might have been missed by some, so sending it again.

I would really appreciate if anybody could guide me in my problem.

Hope to hear people's advices this time.
Thanks

Best,
Arjun
 

Begin forwarded message:

 From: arjun ruhella ruhella.ar...@gmail.com
 Date: October 2, 2012 8:34:59 AM EDT
 To: histonet@lists.utsouthwestern.edu
 Subject: MIXED fiber type identification by Immunohistochemistry 
 
 Greetings to all, 
 
 In immunohistochemistry, I am encountering confusion in designating muscle 
 fibers (based on staining color intensity) to a particular fiber type. 
 
 This problem is particularly bothersome when i encounter the fibers stained 
 with 2 different colors, one color has good intensity while other seems to be 
 of light intensity. I don't have any criterion whether to consider this light 
 staining as actual staining, which would classify the fibers as MIXED fibers 
 or should i consider this light staining as background/non specific staining 
 in which case the fibers will be classified as PURE fibers.   
 
 Does anybody can suggest any criterion/tips they have been using to identify 
 muscle fiber type as correctly as possible? I can understand that identifying 
 MIXED fibers is subjective in immunohistochemistry, but it would be of 
 immense help if i could get to know of any objective suggestions.
 
 
 Animal= spinal cord injured Rat
 Muscle = Soleus
 Section Thickness = 10µm 
 
 I will really appreciate the help! 
 
 Thanks, hope to hear from ppl soon! 
 
 BEST, 
 
 AR 

Best,
Arjun

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RE: [Histonet] Fwd: MIXED fiber type identification by Immunohistochemistry

[Elizabeth Chlipala]

I think you normally use ATPase at different pH's, which is an enzyme stain, to 
differentiate muscle fibers.

At pH 10.2 it would stain Type I fibers light and then Type II Fibers dark  
(all types IIa, IIb and IIc) the other pH's stratify out the type II fibers.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of arjun ruhella
Sent: Monday, October 22, 2012 11:51 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fwd: MIXED fiber type identification by Immunohistochemistry

Dear all,

Its been a while i asked for a doubt to all histology experts around US, but i 
have not been able to get much reply (only 1 :(). I thought my doubt/e mail 
might have been missed by some, so sending it again.

I would really appreciate if anybody could guide me in my problem.

Hope to hear people's advices this time.
Thanks

Best,
Arjun


Begin forwarded message:

 From: arjun ruhella ruhella.ar...@gmail.com
 Date: October 2, 2012 8:34:59 AM EDT
 To: histonet@lists.utsouthwestern.edu
 Subject: MIXED fiber type identification by Immunohistochemistry

 Greetings to all,

 In immunohistochemistry, I am encountering confusion in designating muscle 
 fibers (based on staining color intensity) to a particular fiber type.

 This problem is particularly bothersome when i encounter the fibers stained 
 with 2 different colors, one color has good intensity while other seems to be 
 of light intensity. I don't have any criterion whether to consider this light 
 staining as actual staining, which would classify the fibers as MIXED fibers 
 or should i consider this light staining as background/non specific staining 
 in which case the fibers will be classified as PURE fibers.

 Does anybody can suggest any criterion/tips they have been using to identify 
 muscle fiber type as correctly as possible? I can understand that identifying 
 MIXED fibers is subjective in immunohistochemistry, but it would be of 
 immense help if i could get to know of any objective suggestions.


 Animal= spinal cord injured Rat
 Muscle = Soleus
 Section Thickness = 10µm

 I will really appreciate the help!

 Thanks, hope to hear from ppl soon!

 BEST,

 AR

Best,
Arjun

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[Histonet] Fwd: Announcing the 7th international retreat of applied IHC and molecular pathology (AIMP)

[Richard Cartun]

FYI

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-2204 Fax

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RE: [Histonet] Fwd: Plants

[Susan.Walzer]

I read an article once that said spider plants absorb formalin fumes we have 
kept them in the histo lab for years.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey
Sent: Friday, May 11, 2012 4:07 PM
To: Victoria Baker
Cc: histonet@lists.utsouthwestern.edu; Behnaz Sohrab
Subject: Re: [Histonet] Fwd: Plants

I keep a pothos and spider plant in my lab. EHS has never complained,
though I can't say one way or another if it's technically allowed.

While my plants are mostly just decorative (I don't think I have enough of
them to make much of a difference), it doesn't hurt that they may be
filtering our air somewhat. NASA compiled a list of air-filtering plants
that can eliminate significant amounts of formaldehyde, xylene, benzene,
etc. (Source:   http://en.wikipedia.org/wiki/List_of_air-filtering_plants).

Lucie
UCSD
Dept. of Pathology


On Friday, May 11, 2012, Victoria Baker wrote:

 It's probably more toxic for the plants, but I like having them and no one
 has told me I had to remove them.  Ivy's are the most sturdy and the green
 color just perks up things.
 On Fri, May 11, 2012 at 1:29 PM, Behnaz Sohrab sohra...@ah.org wrote:

 
  I was told by infectious control person that plants are not allowed in
 the
  lab?? IS this true? any experience with this?
  Thank you, Behnaz
 
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Re: [Histonet] Fwd: Plants

[Kim Donadio]

Hey I love having plants in the lab. I just offered up what I've been told by 
other infectious control people. Some infectious control people are more 
serious than others. In the end you'll have to sell that person on it. I've 
seen it turn out both ways. 
Happy week ! 
Kim D

Sent from my iPhone

On May 14, 2012, at 3:25 AM, susan.wal...@hcahealthcare.com wrote:

 I read an article once that said spider plants absorb formalin fumes we have 
 kept them in the histo lab for years.
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey
 Sent: Friday, May 11, 2012 4:07 PM
 To: Victoria Baker
 Cc: histonet@lists.utsouthwestern.edu; Behnaz Sohrab
 Subject: Re: [Histonet] Fwd: Plants
 
 I keep a pothos and spider plant in my lab. EHS has never complained,
 though I can't say one way or another if it's technically allowed.
 
 While my plants are mostly just decorative (I don't think I have enough of
 them to make much of a difference), it doesn't hurt that they may be
 filtering our air somewhat. NASA compiled a list of air-filtering plants
 that can eliminate significant amounts of formaldehyde, xylene, benzene,
 etc. (Source:   http://en.wikipedia.org/wiki/List_of_air-filtering_plants).
 
 Lucie
 UCSD
 Dept. of Pathology
 
 
 On Friday, May 11, 2012, Victoria Baker wrote:
 
 It's probably more toxic for the plants, but I like having them and no one
 has told me I had to remove them.  Ivy's are the most sturdy and the green
 color just perks up things.
 On Fri, May 11, 2012 at 1:29 PM, Behnaz Sohrab sohra...@ah.org wrote:
 
 
 I was told by infectious control person that plants are not allowed in
 the
 lab?? IS this true? any experience with this?
 Thank you, Behnaz
 
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 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 
 
 ___
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 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 
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 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 
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RE: [Histonet] Fwd: Plants

[Stephanie Rivera]

We were told last year to remove all plants from our labsomething about 
contaminants.not sure if it was FDA rule or our department rule, but we had 
to remove all plants and an inspection from the higher ups was done to ensure 
all plants had been removed.



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab
Sent: Friday, May 11, 2012 1:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fwd: Plants


I was told by infectious control person that plants are not allowed in the 
lab?? IS this true? any experience with this?
Thank you, Behnaz


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RE: [Histonet] Fwd: Plants

[Tony Henwood (SCHN)]

Well,

Why are we not surprised that the higher-ups have the authority but little 
knowledge?

At least Stephanie's Bosses decisions cannot be taken as gospel for the rest of 
us! (ie we can ignore them)



Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager  Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stephanie Rivera
Sent: Monday, 14 May 2012 11:11 PM
To: Behnaz Sohrab; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Fwd: Plants

We were told last year to remove all plants from our labsomething about 
contaminants.not sure if it was FDA rule or our department rule, but we had 
to remove all plants and an inspection from the higher ups was done to ensure 
all plants had been removed.



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab
Sent: Friday, May 11, 2012 1:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fwd: Plants


I was told by infectious control person that plants are not allowed in the 
lab?? IS this true? any experience with this?
Thank you, Behnaz


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*
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you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual 
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This note also confirms that this email message has been virus scanned and 
although no computer viruses were detected, The Childrens Hospital at Westmead 
accepts no liability for any consequential damage resulting from email 
containing computer viruses.
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RE: [Histonet] Fwd: Plants

[Tony Henwood (SCHN)]

If referring to a Histopathology lab, what a load of codswallop.

Don't we love supposed Professionals who make decisions based on wrong 
information.

I would suggest that the Infectious Control person do some further study on 
other areas of pathology.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager  Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab
Sent: Saturday, 12 May 2012 3:30 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fwd: Plants


I was told by infectious control person that plants are not allowed in the 
lab?? IS this true? any experience with this?
Thank you, Behnaz

*
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solely for the use of the individual or entity to whom they are addressed. If 
you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual 
sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been virus scanned and 
although no computer viruses were detected, The Childrens Hospital at Westmead 
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containing computer viruses.
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RE: [Histonet] Fwd: Plants

[Tony Henwood (SCHN)]

I would suggest that it is the potting mix that is the culprit not the plant.

But then remember Histo laboratories are not ICUs nor are they Microbiology 
laboratories.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager  Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Donadio
Sent: Saturday, 12 May 2012 6:07 AM
To: William; Behnaz Sohrab
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Fwd: Plants

Hate to say it, but yes plants are considered infectious. Thats why you cant 
take them in ICU's either. I guess the mold or bacterias can grow on them. Most 
places let this slide, but some dont. Good luck! 




From: William cha...@yahoo.com
To: Behnaz Sohrab sohra...@ah.org 
Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
Sent: Friday, May 11, 2012 1:32 PM
Subject: Re: [Histonet] Fwd: Plants

I have had plants in a number of labs. Could be against the rules, but I never 
saw it. I even had a canary in one lab - pretty sure that is against the rules. 

Will Chappell

Sent from my iPhone

On May 11, 2012, at 1:29 PM, Behnaz Sohrab sohra...@ah.org wrote:

 
 I was told by infectious control person that plants are not allowed in the 
 lab?? IS this true? any experience with this?
 Thank you, Behnaz
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you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual 
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[Histonet] Fwd: Plants

[Behnaz Sohrab]

I was told by infectious control person that plants are not allowed in the 
lab?? IS this true? any experience with this?
Thank you, Behnaz
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Re: [Histonet] Fwd: Plants

[William]

I have had plants in a number of labs. Could be against the rules, but I never 
saw it. I even had a canary in one lab - pretty sure that is against the rules. 

Will Chappell

Sent from my iPhone

On May 11, 2012, at 1:29 PM, Behnaz Sohrab sohra...@ah.org wrote:

 
 I was told by infectious control person that plants are not allowed in the 
 lab?? IS this true? any experience with this?
 Thank you, Behnaz
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Re: [Histonet] Fwd: Plants

[Victoria Baker]

It's probably more toxic for the plants, but I like having them and no one
has told me I had to remove them.  Ivy's are the most sturdy and the green
color just perks up things.
On Fri, May 11, 2012 at 1:29 PM, Behnaz Sohrab sohra...@ah.org wrote:


 I was told by infectious control person that plants are not allowed in the
 lab?? IS this true? any experience with this?
 Thank you, Behnaz

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Re: [Histonet] Fwd: Plants

[Lucie Guernsey]

I keep a pothos and spider plant in my lab. EHS has never complained,
though I can't say one way or another if it's technically allowed.

While my plants are mostly just decorative (I don't think I have enough of
them to make much of a difference), it doesn't hurt that they may be
filtering our air somewhat. NASA compiled a list of air-filtering plants
that can eliminate significant amounts of formaldehyde, xylene, benzene,
etc. (Source:   http://en.wikipedia.org/wiki/List_of_air-filtering_plants).

Lucie
UCSD
Dept. of Pathology


On Friday, May 11, 2012, Victoria Baker wrote:

 It's probably more toxic for the plants, but I like having them and no one
 has told me I had to remove them.  Ivy's are the most sturdy and the green
 color just perks up things.
 On Fri, May 11, 2012 at 1:29 PM, Behnaz Sohrab sohra...@ah.org wrote:

 
  I was told by infectious control person that plants are not allowed in
 the
  lab?? IS this true? any experience with this?
  Thank you, Behnaz
 
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Re: [Histonet] Fwd: Plants

[Kim Donadio]

Hate to say it, but yes plants are considered infectious. Thats why you cant 
take them in ICU's either. I guess the mold or bacterias can grow on them. Most 
places let this slide, but some dont. Good luck! 




From: William cha...@yahoo.com
To: Behnaz Sohrab sohra...@ah.org 
Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
Sent: Friday, May 11, 2012 1:32 PM
Subject: Re: [Histonet] Fwd: Plants

I have had plants in a number of labs. Could be against the rules, but I never 
saw it. I even had a canary in one lab - pretty sure that is against the rules. 

Will Chappell

Sent from my iPhone

On May 11, 2012, at 1:29 PM, Behnaz Sohrab sohra...@ah.org wrote:

 
 I was told by infectious control person that plants are not allowed in the 
 lab?? IS this true? any experience with this?
 Thank you, Behnaz
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RE: [Histonet] Fwd: Plants

[Sebree Linda A]

I've always had at least one; makes the day more tolerable. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Behnaz
Sohrab
Sent: Friday, May 11, 2012 12:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fwd: Plants


I was told by infectious control person that plants are not allowed in
the lab?? IS this true? any experience with this?
Thank you, Behnaz

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RE: [Histonet] Fwd: Plants

[Sebree Linda A]

Yeah but they used to use canaries in mines to warn of toxic levels of
gases; having one in a histo lab might be a VERY good idea! 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of William
Sent: Friday, May 11, 2012 12:33 PM
To: Behnaz Sohrab
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Fwd: Plants

I have had plants in a number of labs. Could be against the rules, but I
never saw it. I even had a canary in one lab - pretty sure that is
against the rules. 

Will Chappell

Sent from my iPhone

On May 11, 2012, at 1:29 PM, Behnaz Sohrab sohra...@ah.org wrote:

 
 I was told by infectious control person that plants are not allowed in
the lab?? IS this true? any experience with this?
 Thank you, Behnaz
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 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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Re: [Histonet] Fwd: Plants

[Emily Sours]

A canary? Well, I know what you used that for...how did that work out for
you?
Actually, I know the answer because you're alive.
EHS should really look into this option, screw the badges...

Emily

The whole point of this country is if you want to eat garbage, balloon up
to 600 pounds and die of a heart attack at 43, you can! You are free to do
so. To me, that’s beautiful.
--Ron Swanson



On Fri, May 11, 2012 at 1:32 PM, William cha...@yahoo.com wrote:

 I have had plants in a number of labs. Could be against the rules, but I
 never saw it. I even had a canary in one lab - pretty sure that is against
 the rules.

 Will Chappell

 Sent from my iPhone

 On May 11, 2012, at 1:29 PM, Behnaz Sohrab sohra...@ah.org wrote:

 
  I was told by infectious control person that plants are not allowed in
 the lab?? IS this true? any experience with this?
  Thank you, Behnaz
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[Histonet] Fwd: AADA rapid response team convinces Aetna to clarify policy on accreditation for in-office pathology labs

[Nicole Tatum]

 Original Message 
Subject: Fwd: AADA rapid response team convinces Aetna to clarify policy
on accreditation for in-office pathology labs
From:blondi33...@aol.com
Date:Tue, May 8, 2012 3:57 pm
To:  nic...@dlcjax.com
--





-Original Message-
From: nfe1244 nfe1...@aol.com
To: seaglstein seaglst...@gmail.com; pam p...@dlc.net; blondi1
blondi33...@aol.com
Sent: Sun, May 6, 2012 9:06 am
Subject: Fwd: AADA rapid response team convinces Aetna to clarify policy
on accreditation for in-office pathology labs


good news!



-Original Message-
From: American Academy of Dermatology Association
nore...@aadassociation.org
To: N. Fred Eaglstein; DO; FAAD nfe1...@aol.com
Sent: Fri, May 4, 2012 5:58 pm
Subject: AADA rapid response team convinces Aetna to clarify policy on
accreditation for in-office pathology labs


If you can't see the images in this email, please click here.













This week’s headlines:


Indoor tanning bed labeling legislation introduced, fails to include FDA
reclassification
House and Senate Committees continue working on prescription drug, medical
device legislation
AADA provides comments to the FDA regarding biosimilars
AADA rapid response team convinces Aetna to clarify policy on
accreditation for in-office pathology labs
Dermatology societies to collaboratively share recently approved AUC for
Mohs surgery with payers
Vermont becomes second state in the nation to ban tanning for minors
California patient safety bill moves swiftly through state assembly
Cosmetic tax proposals stripped from California bills
Tanning bill endorsed by Missouri House of Representatives
Mississippi enacts ‘Patient’s Right to Informed Health Care Choices Act’
Maryland enacts board certification disclosure requirements
SkinPAC to host fundraiser in Chicago
Register now for the 2012 AADA Legislative Conference, Sept. 9 – 11,
Washington D.C.


Congressional action


Indoor tanning bed labeling legislation introduced, fails to include FDA
reclassification
On April 19, Sens. Jack Reed (D-R.I.) and Johnny Isakson (R-Ga.)
introduced S. 2301, the Tanning Transparency and Notification Act of 2012
which calls on the FDA to enhance indoor tanning bed labeling requirements
based on recommendations the agency made as a result of the TAN Act of
2006. The AADA has been working closely with Sen. Reed’s office to
introduce a Senate companion bill to H.R. 1676, the Tanning Bed Cancer
Control Act, which calls on the Food and Drug Administration (FDA) to
reclassify indoor tanning beds, however Sen. Reed introduced his new
Senate legislation removing the AADA-supported FDA reclassification
language and leaving only the enhanced labeling portion of the bill. The
AADA sent a letter to both senators stating the importance of including
language calling on the FDA to reclassify tanning beds, in addition to the
enhanced labeling requirements that, alone, do little to deter the use of
indoor tanning beds.
House and Senate committees continue working on prescription drug, medical
device legislation
The House Energy  Commerce Committee and the Senate Health, Education,
Labor, and Pensions (HELP) Committee are working on a bipartisan effort to
reauthorize prescription drug and medical device user fee legislation. The
resulting bills are expected to come to the House and Senate floors for
action sometime in June. Both the House and Senate draft bills include
language to address the ongoing prescription drug shortages that
physicians across the country are facing and propose solutions to mitigate
future shortages. Additionally, the Senate HELP Committee version
currently includes legislation (S. 2301) introduced by Sens. Jack Reed
(D-R.I.) and Johnny Isakson (R-Ga.) calling on the FDA to enhance indoor
tanning bed labeling requirements based on the agency’s recommendations
(see story above). As the Committee process moves forward, the AADA is
monitoring the legislation and urging Congress to also include language
calling on the FDA to reclassify indoor tanning beds.


Federal agency focus


AADA provides comments to the FDA regarding biosimilars
On May 11, the Food and Drug Administration will convene a public hearing
regarding biosimilars. The hearing will include conversations on naming,
labeling, and pharmacovigilance of these new therapies. In anticipation of
the hearing, the AADA has submitted comments to the agency highlighting
our continued concerns regarding naming and pharmacoviligance issues. The
Academy urges the agency to provide unique non-proprietary names for all
biosimilars to reduce any confusion with the reference biologic products.
An update on the May 11 meeting will appear in the next issue of
Dermatology Advocate.


Private payer activity


AADA rapid response team convinces Aetna to clarify policy on
accreditation for in-office pathology labs
Aetna has 

[Histonet] Fwd:

[Tuyen Nguyen]

Greetings, friend!  
http://www.fminformatica.com/like.php?wutyg=44ocugimuv=215ivozuvitu=99

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[Histonet] Fwd: Rapid MART-1 procedure

[Charity Wyatt]

-- Forwarded message --
From: Charity Wyatt charchar...@gmail.com
Date: Wed, Jan 18, 2012 at 9:01 AM
Subject: Rapid MART-1 procedure
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu


Hi All! I work for a busy Mohs surgeon that wants to start doing a rapid
MART-1 (Melan-A) immunostain with our melanoma cases.  Does anyone have any
suggestions/protocols/vendor names to help me get started?  I'm starting
from the ground up here, we don't currently do any immuno stains.  We will
be doing the stains on frozen sections, and they will be done manually.
Any tips would be greatly appreciated!  Thanks!

-- 
Charity Wyatt
Mohs Histotechnologist
Jacksonville Skin Cancer Center, P.A./
Michael E. Lutz, M.D.
(904)737-0111




-- 
Charity Wyatt
Mohs Histotechnologist
Jacksonville Skin Cancer Center, P.A./
Michael E. Lutz, M.D.
(904)737-0111
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[Histonet] Fwd: IHC control

[Marian Powers]

-Marian 


Begin forwarded message:

 From: Marian Powers mpow...@dpspa.com
 Date: November 16, 2011 12:51:56 PM EST
 To: histonet@lists.utsouthwestern.edu
 Subject: IHC control
 

 Hi:  Would anyone have a hereditary nonpolyposis colorectal cancer control, 
 (for MHL1, MSH2, MSH6) to share?
  
 Thanks in advance,
 
 -- 
  
 Marian L. Powers 
  
 Doctors Pathology Services 
 c| 302.747.0580
 o| 302.677. ext: 110
 f | 302.677.0010
  
  
  
  
  
  
  
  
 
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[Histonet] Fwd: Formic Acid Recipe

[Jesus Hernandez]

Begin forwarded message:

 From: Jesus Hernandez jesus.w@gmail.com
 Date: November 4, 2011 4:41:20 PM CDT
 To: histonet@lists.utsouthwestern.edu
 Subject: Formic Acid Recipe
 
 Dear all,
 
 I was having trouble staining Human embryonic palatal mesenchymal cells with 
 multi-stain solution. My PI told me to use formic acid on the samples so that 
 it could increase the permeability of the methyl methacrylate. I am not sure 
 if this is enough information, but the cells were loaded on hydroxyapatite 
 scaffolds. I have also tried aniline blue and villaneuva stain. Any 
 information on how I can make a solution of formic acid is appreciated along 
 with maybe other stains I could try using. Each sample was grinded to about 
 50 microns. Thank you.
 
 Best Regards,
 
  Jesus W. Hernandez
 Graduate Student
 Department of Biomedical Engineering
 University of Texas at San Antonio
 jesus.w@gmail.com
 
 
 

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[Histonet] Fwd:

[lynn13361]

http://hcauto.com/interesting.php?id=14



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[Histonet] Fwd: Re: Monday deployed changes to worklist processing

[Maureen Griffo]

All,

Jeff's email below is a message that they have successfully re-directed 
the ICC recut (automatic HE request) to the IP worklist now.  The 
unstained slides have also been re-directed there as the majority of 
these requests are for IHC and or FISH.  This lets all have the same 
visual around the unstained slides that were ordered and cut.


Both teams need to keep your eyes on this and get feedback to us 
promptly if you see any issues.  Thanks much!  Let me know of your 
questions.


Maureen

 Original Message 
Subject:Re: Monday deployed changes to worklist processing
Date:   Fri, 26 Aug 2011 10:52:59 -0700
From:   Jeff Cochran j...@pathology.washington.edu
To: Maureen Griffo mgri...@u.washington.edu



Yep. You coming here? Me go to your place?

On 8/26/2011 10:41 AM, Maureen Griffo wrote:

 Hi Jeff,

 Do you have a moment to chat about this in ~15 minutes?

 Thanks,

 Maureen

 On 8/25/2011 9:19 AM, Jeff Cochran wrote:

 Maureen,

 Monday morning (while the Bond is being restarted), we'll deploy the
 alterations to the Bond interface that allow:

 1) Non-staining orders to appear on a worklist monitored for the
 Bond. Orders ICC US and ICC Recut will appear on the IP Worksheet
 worklist with no messages directed to the Bond. Worklist modification
 to orders for tests on the Bond will have the same affect as current.
 For example, moving an order from IP Worksheet to another ICC
 worklist will send a cancel-order message to the Bond (common for
 managing research cases). Worklist modification to orders for tests
 not implemented on the Bond will behave correctly (e.g., moving an
 order for FISH to the IP Worksheet worklist will not cause a message
 to be sent to the Bond).

 2) Procedures configured both by worklist (in routing) and through
 declaration of a protocol name for the Bond. The protocol name is
 the Bond's public name for a test. The feature allows multiple LIS
 procedures mapped to a single Bond test, supporting the CAP-directed
 alterations in resulting for breast panel tests.

 As part of the rework to add these new features, significant work has
 been done to increase stability and scalability in the Bond
 interface, and to access more LIS data when auto-generating negative
 control and HE orders.

 Please let everyone know and please ask that they keep their eyes
 open for any problems on Monday. I'll be keeping the day open for any
 problems that may arise and the team should feel free to grab me or,
 preferably, open a high-priority tech request if they need to.

 Thanks,

 Jeff



--
Jeff Cochran
Senior Computer Specialist
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
(206) 598-1616
(206) 598-7659 Fax
=
Privileged, confidential or patient identifiable information may be
contained in this message. This information is meant only for the use
of the intended recipients. If you are not the intended recipient, or
if the message has been addressed to you in error, do not read,
disclose, reproduce, distribute, disseminate or otherwise use this
transmission. Instead, please notify the sender by reply e-mail, and
then destroy all copies of the message and any attachments.


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[Histonet] Fwd: Article on the Tornado in the Joplin hospital

[Reuel Cornelia]

A must read article on code Gray. Very sad story but heroic. 

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[Histonet] Fwd: GMS counterstain

[Cheryl Crowder]

Ally - I have worked with several pathologists who were color-blind -one to 
greens; one to blues.  As a result we counterstained the GMS with different 
colors.  A counterstain is just that.  It gives a color to secondary tissue 
elements.  Light greens, hematoxylin, metanil yellow and nuclear fast red 
were used. All worked well, photographed well and were the choice of the 
pathologist.  Many older pathologists grew up with the procedure outlined 
in the AFIP manual which stated green.  But other pathologists are more 
amiable to trying other colors.  Maybe you should  just as them what they 
prefer.
Cheryl
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[Histonet] Fwd: Nor Cal Histotech program

[Jonathan Krupp]

Hi

I am new here. I am part of an EM tech training program at Delta College in 
Stockton, CA.

We train students to do EM, fixing, sectioning, staining, and instrument 
operation.

Some of my students have been asking about LM histotech sort of things. They 
want to know more about histo techniques in general and maybe broadening their 
job prospects beyond EM.

I am exploring the possibilities of adding more LM histotech things to the 
program and seek advice. Is there room for another program? Is seeking 
accreditation worth while? What would I need to get set up? Are there jobs 
available for graduates?

Just getting started, your advice will be invaluable.

Jon

Jonathan Krupp
Delta College
5151Pacific Ave.
Stockton, CA  95207
209-954-5284
jkr...@deltacollege.edu





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Re: [Histonet] Fwd: Nor Cal Histotech program

[Lee Peggy Wenk]

There is SUCH a need for histotech programs. Currently, there are only 39 
histologic technician (HT) (most community college based) and 4 
histotechnologist (HTL) (need 4 year degree). And there are shortages 
everywhere.


There are 2 HT programs in California. One in Berkley, one in Walnut.

The accrediting agency is National Accrediting Agency for Clinical 
Laboratory Sciences (NAACLS).

www.naacls.org

For information on how to contact the two California programs, once on the 
NAACLS website,

- on the left, click on Find a Program
- Under Program Type, click on HT  hit Search. Programs are listed 
alphabetically by state.
- If you want to talk with HTL program directors, follow above directions, 
except click on HTL.


Any program director would be willing to give you more information.

If you look under accreditation on the left, there is HT and HTL. Click on 
one, and you can find the accreditation standards you would have to follow. 
There are a few standards that are different between HT and HTL, so you need 
to look at both.


The advantage of being an accredited HT or HTL program is that the students 
are eligible to take the national certification exam through American 
Society for Clinical Pathology (ASCP), immediately upon successful 
completion of the NAACLS accredited HT or HTL program.


However, since you are just starting out, and look like you need lots of 
information, I'm going to suggest going to the National Society for 
Histotechnology (NSH) website. More information about what histotechs are, 
about meetings, state society presidents, jobs board, membership with 
journal specific for histology/tissue, etc.


There is also a 3 hour continuing education you can purchase, titled How to 
Start an Accredited HT or HTL Program in either a College or a Hospital. I 
presented it at the NSH Symposium in 2008, and it was recorded with the 
PowerPoint, so you can listen to me and look at the PowerPoint. It has lots 
of advantages and disadvantages of starting different types of programs, 
contacts, proof that we need more programs, etc. Granted, the information is 
3 years old, and there are a lot more HT and HTL program than there were 3 
years ago, but most of the other information is the same. Just a disclaimer, 
I don't make any money from this. I had presented it every year from 
1999-2008, and NSH thought it might work as an on-line module, for people 
like yourself who need the information at times other than in the fall when 
the NSH symposium is.


Go to www.nsh.org
- Across the top, click on Professional Development
- About the 5th one down, click on On-Line Learning Center
- Click on Meeting Content
- Scroll down and click on NSH 2008 Annual Symposium/Convention in 
Pittsburgh, PA

- Click on #27 = How to Start an Accredited HT or HTL program

Once you get some information under your belt, I'd be more than happy to 
answer questions specific for your situation. Just look on the NAACLS web 
page, under HT or HTL (we have both programs), and you'll find me in 
Michigan.   :-)


Peggy A. Wenk, HTL(ASCP)SLS
Schools of Histotechnology
William Beaumont Hospital
Royal Oak, MI 48073

--
From: Jonathan Krupp jkr...@deltacollege.edu
Sent: Tuesday, December 28, 2010 6:22 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fwd: Nor Cal Histotech program





Hi

I am new here. I am part of an EM tech training program at Delta College 
in Stockton, CA.


We train students to do EM, fixing, sectioning, staining, and instrument 
operation.


Some of my students have been asking about LM histotech sort of things. 
They want to know more about histo techniques in general and maybe 
broadening their job prospects beyond EM.


I am exploring the possibilities of adding more LM histotech things to the 
program and seek advice. Is there room for another program? Is seeking 
accreditation worth while? What would I need to get set up? Are there jobs 
available for graduates?


Just getting started, your advice will be invaluable.

Jon

Jonathan Krupp
Delta College
5151Pacific Ave.
Stockton, CA  95207
209-954-5284
jkr...@deltacollege.edu





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[Histonet] Fwd: Physician Signature Requirement Update

[Richard Cartun]

FYI

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


 Denise Bologna 12/21/2010 5:27 PM 
FYI

 CLMA i...@clma.org 12/21/2010 3:44 PM 

 ( 
http://www.clma.org/link.asp?ymlink=406069finalurl=http%3A%2F%2Fwww%2Eclma%2Eorg
 )

Physician Signature Requirement Update 

Centers for Medicare  Medicaid Services (CMS) has issued a temporary 
(approximately three month) delay on the implementation of the Physician 
Signature Requirement. 

Please check back with CLMA ( 
http://www.clma.org/link.asp?ymlink=406069finalurl=http%3A%2F%2Fwww%2Eclma%2Eorg
 ) often for updated information and read more ( 
http://www.clma.org/link.asp?ymlink=406069finalurl=http%3A%2F%2Fwww%2Ecms%2Egov%2FClinicalLabFeeSched%2F
 ) at the CMS website. 




 ( 
http://www.clma.org/link.asp?ymlink=406069finalurl=http%3A%2F%2Fwww%2Eclma%2Eorg
 )


This email was sent to 'dbol...@clpct.com' from CLMA - Clinical Laboratory 
Management Association. 
If you wish to stop receiving email from us, you can simply remove yourself by 
visiting: http://clma.site-ym.com/general/opt.asp?e=dbol...@clpct.com


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[Histonet] Fwd: IHC coverglassing

[Jen Campbell]

Jen Campbell
Muhlbauer Dermatopathology Laboratory
Supervisor of Technical Services
Phone 585-586-5166
Fax 585-586-3137

- Forwarded Message -
From: Jen Campbell jcampbell_...@frontier.com
To: histonet histonet-boun...@lists.utsouthwestern.edu
Sent: Tuesday, October 19, 2010 11:16:38 AM
Subject: IHC coverglassing

Greetings to all the histonet followers!!

A question was recently brought forth in the lab I work at. Can non-automated 
IHC slides be dehydrated and cleared(xylene substitute) after staining and be 
placed on an automated coverglasser?

Jen Campbell
Muhlbauer Dermatopathology Laboratory
Supervisor of Technical Services
Phone 585-586-5166
Fax 585-586-3137

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RE: [Histonet] Fwd: IHC coverglassing

[Thomas, Nancy]

Hi Jen,
We do it that way routinely.  No problems.
Nancy

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jen Campbell
Sent: Tuesday, October 19, 2010 12:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fwd: IHC coverglassing




Jen Campbell
Muhlbauer Dermatopathology Laboratory
Supervisor of Technical Services
Phone 585-586-5166
Fax 585-586-3137

- Forwarded Message -
From: Jen Campbell jcampbell_...@frontier.com
To: histonet histonet-boun...@lists.utsouthwestern.edu
Sent: Tuesday, October 19, 2010 11:16:38 AM
Subject: IHC coverglassing

Greetings to all the histonet followers!!

A question was recently brought forth in the lab I work at. Can non-automated 
IHC slides be dehydrated and cleared(xylene substitute) after staining and be 
placed on an automated coverglasser?

Jen Campbell
Muhlbauer Dermatopathology Laboratory
Supervisor of Technical Services
Phone 585-586-5166
Fax 585-586-3137

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RE: [Histonet] Fwd: IHC coverglassing

[Houston, Ronald]

absolutely

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jen Campbell
Sent: Tuesday, October 19, 2010 1:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fwd: IHC coverglassing



Jen Campbell
Muhlbauer Dermatopathology Laboratory
Supervisor of Technical Services
Phone 585-586-5166
Fax 585-586-3137

- Forwarded Message -
From: Jen Campbell jcampbell_...@frontier.com
To: histonet histonet-boun...@lists.utsouthwestern.edu
Sent: Tuesday, October 19, 2010 11:16:38 AM
Subject: IHC coverglassing

Greetings to all the histonet followers!!

A question was recently brought forth in the lab I work at. Can non-automated 
IHC slides be dehydrated and cleared(xylene substitute) after staining and be 
placed on an automated coverglasser?

Jen Campbell
Muhlbauer Dermatopathology Laboratory
Supervisor of Technical Services
Phone 585-586-5166
Fax 585-586-3137

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RE: [Histonet] Fwd: IHC coverglassing

[sgoebel]

Absolutely!!  After the buffer rinse (after the counterstain), rinse in
water, then 95, then 100, then xylene, and coverslip away!!  I do it
everyday and it works fine =)


Sarah Goebel, B.A., HT (ASCP)

Histotechnician


XBiotech USA Inc.

8201 East Riverside Dr. Bldg 4 Suite 100

Austin, Texas  78744

(512)386-2907




 Original Message 
Subject: [Histonet] Fwd: IHC coverglassing
From: Jen Campbell jcampbell_...@frontier.com
Date: Tue, October 19, 2010 10:16 am
To: histonet@lists.utsouthwestern.edu



Jen Campbell
Muhlbauer Dermatopathology Laboratory
Supervisor of Technical Services
Phone 585-586-5166
Fax 585-586-3137

- Forwarded Message -
From: Jen Campbell jcampbell_...@frontier.com
To: histonet histonet-boun...@lists.utsouthwestern.edu
Sent: Tuesday, October 19, 2010 11:16:38 AM
Subject: IHC coverglassing

Greetings to all the histonet followers!!

A question was recently brought forth in the lab I work at. Can
non-automated IHC slides be dehydrated and cleared(xylene substitute)
after staining and be placed on an automated coverglasser?

Jen Campbell
Muhlbauer Dermatopathology Laboratory
Supervisor of Technical Services
Phone 585-586-5166
Fax 585-586-3137

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[Histonet] Fwd: [PPMA] See OmniTrax at UWMC

[Victor Tobias]

 For any of you PowerPath users or anyone else curious about barcoding 
workflow, that are visiting Seattle, please see the message below. You 
may reply directly to Dr. Schmidt.


Victor

 Original Message 
Subject:[PPMA] See OmniTrax at UWMC
Date:   Thu, 23 Sep 2010 10:31:29 -0700 (Pacific Daylight Time)
From:   Rodney Schmidt schmi...@u.washington.edu
Reply-To: 	A mutual assistance forum for PowerPath users 
p...@u.washington.edu

To: PowerPath Mutual Assistance p...@u.washington.edu



There are several events in the next few weeks that may be bringing
PowerPath users to Seattle.  If any of you would like to visit the
University of Washington Medical Center to see the best workflow-driving
and comprehensive barcoding solution available anywhere (OmniTrax), please
send me a private email.  We’re anticipating being able to host tours on
Monday, Tuesday and Wednesday during NSH and can work with your schedule
if you’re in the area for other reasons (e.g. a users conference).


Rodney A. Schmidt, M.D., Ph.D.
Professor of Pathology
Director of Medical Informatics
University of Washington, Seattle, WA
(206) 598-6462
(206) 344-0532 (pager)
(206) 598-3803 (fax)

=
Privileged, confidential or patient identifiable information may be
contained in this message. This information is meant only for the use of
the intended recipients. If you are not the intended recipient, or if the
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Instead, please notify the sender by reply e-mail, and then destroy all
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[Histonet] Fwd: TDE decalcifier, decalcification commentary

[louise renton]

 Thanks Gayle for your input.

Trouble is, i like the TDE system, and despite not knowing what's in the
solution the IHC results are great. I wasn't sure if endpoint testing could
be used with other acids besides HCl, but you have answered my doubts on
that score. I suspect that, from the smell, it is HCl, but I will have to
dig out old chemistry books to remember how to determine an unknown acid!

anyway, thanks again for your invaluable advice.

best regards






On Tue, May 18, 2010 at 8:52 PM, gayle callis gayle.cal...@bresnan.netwrote:

  Hi Teri and Louise,



 There is NO way to tell if a decalcifying solution is used up but you can
 be sure it IS used up.  So the best thing to do is endpoint testing to know
 when the bone is free of calcium.  All decalcifiers become exhausted, so one
 has to replenish the solution with fresh throughout the decal procedure,
 faithfully.  This really doesn't take that much time in the long run, and
 guarantees properly decalcified bone that will not be overexposed to acid -
 a huge no no for decent staining.



 The weight loss/weight gain method is great if you don't have an xray
 machine.   If the solution is an acid e.g. nitric, HCl, or formic, then one
 should do the chemical test or you can play with the weight loss/weight gain
 method.



 Attached are both the chemical and weight loss/weight gain methods, the
 latter was developed for testing nitric acid.  However, this is the easiest
 method to test EDTA other than an xray machine, with the latter being the
 most sensitive i.e. accurate.



 *If the company cannot provide the answer for what is in TDE, then I would
 not use it*. MSDS sometimes tell you, but if not, that company would be
 sayonara from my lab.   I have to know WHAT chemical is doing the work, very
 important to do IHC or routine only staining.   I would rather make it up
 decalcifying solutions myself than buy an product that is unknown, and
 stubbornly proprietary!



 If you do a chemical endpoint test, do NOT stir the decal solution during
 decalcification.  Suspend bones, then collect the aliquot from bottom of the
 container where Ca++ resides.  Remove your samples, and rinse with tap water
 while you do the test.  Return samples to FRESH decalcifying solution and
 proceed with decalcification/testing, etc, etc.



 May not be the answers you wanted but certainly how I have always done bone
 decalcification without problems.



 Take care, Ladies



 Gayle Callis









 *From:* Johnson, Teri [mailto:t...@stowers.org]
 *Sent:* Tuesday, May 18, 2010 9:58 AM
 *To:* 'gayle callis'
 *Subject:* Histonet post



 Hey Gayle, thought I'd point this one out to you. Looks like it's right up
 your alley.



 Message: 1

 Date: Mon, 17 May 2010 19:30:30 +0200

 From: louise renton louise.ren...@gmail.com

 Subject: [Histonet] TDE decalcifier

 To: histonet@lists.utsouthwestern.edu

 Message-ID:

 aanlktinrmd_lrg8otwuashunfc-unuv5x8fjviudy...@mail.gmail.com

 Content-Type: text/plain; charset=ISO-8859-1



 hi all,

 although I have been using the TDE decalcification system for awhile, I was

 hoping to get some answers from the community. are there any thoughts as to

 what the solution is?. And how do you tell if  the solution is used up or

 not. I wonder if anybody out there has some idea as to whether the solution

 is saturated with calcium salt or not. as always, I look forward to some

 answers.

 Best regards

 --

 Louise Renton

 Bone Research Unit

 University of the Witwatersrand

 Johannesburg

 South Africa

 +27 11 717 2298 (tel  fax)

 073 5574456 (emergencies only)

 There are nights when the wolves are silent and only the moon howls.

 George Carlin

 No trees were killed in the sending of this message.

 However, many electrons were terribly inconvenienced.





 I hope things are thawing a bit where you are. I wish it would quit raining
 and warm up here. Supposed to be 80 degrees this weekend. I can't wait!



 All the best,

 Teri





 __ Information from ESET Smart Security, version of virus signature
 database 5122 (20100517) __

 The message was checked by ESET Smart Security.

 http://www.eset.com


 __ Information from ESET Smart Security, version of virus signature
 database 5122 (20100517) __

 The message was checked by ESET Smart Security.

 http://www.eset.com




-- 
 Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel  fax)
073 5574456 (emergencies only)
There are nights when the wolves are silent and only the moon howls.
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.



-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel  fax)
073 5574456 (emergencies only)
There are nights when the wolves are silent and only the moon howls.
George Carlin
No trees were killed in the sending of 

[Histonet] Fwd: New email address !!!

[Dianne Holmes]

 Dianne Holmes 4/27/2010 2:46 PM 


 Dianne Holmes 4/27/2010 2:45 PM 
UMC has changed carriers and now I have a new email address.  It is very close 
to the old one so make sure you note the difference.  Please REPLY to this 
mailing so I will know it is working.  www.dhol...@umc.edu  




Individuals who have received this information in error or are not authorized 
to receive it must promptly return or dispose of the information and notify the 
sender. Those individuals are hereby notified that they are strictly prohibited 
from reviewing, forwarding, printing, copying, distributing or using this 
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[Histonet] Fwd: [PPMA] Barcoding in Boston!

[Victor Tobias]

For any of you in the Boston area, my boss will be speaking on barcoding.

Victor

 Original Message 
Subject:[PPMA] Barcoding in Boston!
Date:   Thu, 15 Apr 2010 10:34:31 -0700 (Pacific Daylight Time)
From:   Rodney Schmidt schmi...@u.washington.edu
Reply-To: 	A mutual assistance forum for PowerPath users 
p...@u.washington.edu

To: PowerPath Mutual Assistance p...@u.washington.edu



The topic of barcoding in pathology has suddenly gotten extremely popular.
Just about all the hardware and LIS exhibitors at the recent USCAP meeting
featured their capabilities.  Not all solutions are equal but it takes a
knowledgeable user to tell the difference between the solutions that are
good for users vs the ones that are good for the vendors.

Most of you know that the University of Washington has built out a
complete, sophisticated, and highly user-friendly barcoding and
workflow-driving system that handles all pathology materials (OmniTrax).
It's being used at multiple academic and private practices (PowerPath
sites) and has recently been revised so that it can work with any LIS.

Our experience with OmniTrax has let us understand deeply exactly what
makes a barcoding system successful from a user's perspective, including
likely benefits to labs in terms of cost savings and error reduction.  In
the last year, I've been invited to give talks about this at APIII, USCAP,
NSH, and AAPA meetings, as well as at other sites.

Thermo Scientific has asked me to speak on this topic next Wednesday in
Boston.  If you're interested and in the area, please contact your Thermo
rep for information.


Rod

Rodney A. Schmidt, M.D., Ph.D.
Professor of Pathology
Director of Medical Informatics
University of Washington, Seattle, WA
(206) 598-6462
(206) 344-0532 (pager)
(206) 598-3803 (fax)

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Privileged, confidential or patient identifiable information may be
contained in this message. This information is meant only for the use of
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Instead, please notify the sender by reply e-mail, and then destroy all
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[Histonet] Fwd: spinal cord paraffin embedding

[Elisa Ballarini]

   Hi.
   First of all I would like to thank all of you for your advices.
   Because,  my  previous  e-mail  wasn't  complete  and to avoid further
   suggestions  regarding  protocols  I can not follow, here I write some
   more information.
   First  of  all,  we  can't perfuse our rats. Also, when we harvest rat
   spinal cord (cut in half, thus around 3mm diameter) we fix it only for
   4-5  hours  in  4%  paraformaldehyde because we need to perform an IHC
   essay so we would like to avoid epitope masking.
   We  don't  think  fixation is the problem since frozen sections of the
   same fixed spinal cords do not show any problem in the white matter.
   In  conclusion,  we need to obtain good results using paraffin both in
   term= s of morphology and IHC staining.
   Our paraffin embedding machine program is as follows:
   Ethanol 95%   1h
   Ethanol 95%   45'
   Ethanol 95%   45'
   Ethanol 100%  45'
   Ethanol 100%  1h
   Ethanol 100%  1h
   Ethanol 100%  1h
   Xylol 45'
   Xylol 1h
   Xylol 1h
   Paraffin 1h
   Paraffin 1h
   Paraffin 1h
   Can  you  tell  me  if  are there any specific protocols to embedd rat
   spinal = cord in paraffin to avoid washing out of myelin?
   Thank you.
   Regards
   --
   -- 
   Dott.ssa Elisa Ballarini,PhD student
   Dipartimento di Neuroscie= nze e Tecnologie Biomediche
   Università degli Studi Milano-Bicocca
   Via Cadore 48-20052,Monza,MB
   e.ballari...@campus.uni= mib.it
   Tel. 02-64488119
   Fax. 02-64488253

--- segue il m= essaggio inoltrato ---
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[Histonet] Fwd: Re: Stains for Macrophages for Laser Capture Microdissection purpose

[John Kiernan]

- Original Message -
From: Christopher Pin c...@uwo.ca
Date: Thursday, January 28, 2010 9:22
Subject: Re: Stains for Macrophages for Laser Capture Microdissection purpose
To: Martin Sandig martin.san...@schulich.uwo.ca, John Kiernan 
jkier...@uwo.ca, Paul Walton pwal...@uwo.ca


 Hi all,
 
 Thanks for including me in these discussions.  I am no expert on macrophage 
 histology but I know something about laser capture microdissection.  The key 
 to this method for RNA isolation is not so much in the timing but rather in 
 the solutions complete lack of RNAse.  We have worked with pancreatic tissue 
 and been able to dissect of cells for RNA isolation.  Likely one approach 
 would be to do HE for identification.  Before you went to the trouble of 
 doing LCM, you could take some unimportant tissue, scrape the tissue off the 
 slide after fixation and then isolate RNA just to verify the integrity of the 
 RNA following staining.
 
 The other thing that you can do is fix the slides as if you are doing in situ 
 hybridization before you do any histology.
 
 I know this isn’t that helpful but from my experience it is not the time but 
 rather the quality of the solutions that makes all the difference.
 
 Chris
 
 
 On 1/28/10 9:01 AM, Martin Sandig martin.san...@schulich.uwo.ca wrote:
 

 Hi:
 I do not do laser capture at all.
 For identification purposes, perhaps it would be possible to load the 
 macrophages with an injected dye (they eat that stuff depending on the tissue 
 under investigation) and then capture them.  In atherosclerotic lesions they 
 are loaded with lipids (foam cells) and perhaps with an excellent microscope 
 they may be visible before capture.
 Chris Pin (c...@uwo.ca) may have some experience with laser micro-dissection.
 Cheers,
 Martin
  
  
 Martin Sandig, PhD
 Associate Professor
 Associate Chair, Undergraduate Affairs
 Department of Anatomy and Cell Biology
 Schulich School of Medicine and Dentistry
 University of Western Ontario
 Dental Sciences Building, Room 00075
 Phone: 519 661 2111 86815
 Fax: 519 850 25662
 http://www.uwo.ca/anatomy/department/sandigm/msandig.html
  
 
 
  Paul Walton pwal...@uwo.ca 28/01/2010 8:24 am 
 John,
 I have forwarded this message on to Martin, who does laser capture. Alas, I 
 am the microinjection fellow.
 Paul
 
 Begin forwarded message:
 

 From: John Kiernan jkier...@uwo.ca
 Date: January 27, 2010 10:41:15 PM EST (CA)
 To: delphine eberle eberledelph...@hotmail.com
 Cc: sharon.will...@bms.com, histonet@lists.utsouthwestern.edu, pwal...@uwo.ca
 Subject: Re: Stains for Macrophages for Laser Capture Microdissection purpose
 
 How about phase contrast optics and identifying the cells by their shapes? 
 Non-specific addition of colour to a section of unfixed tissue probably will 
 not show the cells any more clearly. I have taken the liberty of including a 
 colleague, Dr Paul Walton, in this reply. He does laser capture 
 microdissection and may have something to suggest.
  
 John Kiernan
 Anatomy, UWO
 London, Canada
 = = =
 - Original Message -
 From: delphine eberle eberledelph...@hotmail.com
 Date: Wednesday, January 27, 2010 16:27
 Subject: Stains for Macrophages for Laser Capture Microdissection purpose
 To: jkier...@uwo.ca, sharon.will...@bms.com
 Cc: histonet@lists.utsouthwestern.edu

  Hi,
   
  I have another question following Macrophage staining.
  I am setting up a Laser Capture Microdissection protocol to dissect 
  macrophages from atherosclerosis lesions (non fixed frozen sections) and 
  extract RNA for gene expression purpose.
  I am looking at a quick staining (less than 30min otherwise RNA is 
  degradated) for macrophages in that context? Any suggestions?
   
  Thanks a lot,
  Delphine
  
  Delphine Eberlé PhD 
  UCSF Department of Vascular Surgery
  eberledelph...@hotmail.com java_script:main.compose('new', 
  't=eberledelph...@hotmail.com')  
  
   From: jkier...@uwo.ca
   To: sharon.will...@bms.com
   Date: Wed, 27 Jan 2010 00:22:53 -0500
   Subject: Re: [Histonet] Histology Special Stains for Macrophages
   CC: histonet@lists.utsouthwestern.edu
   
   None of the methods mentioned in the enquiry are stains for macrophages. 
   Research workers who never took Histology 101 often stain cells of the 
   monocyte/macrophage lineage immunohistochemically (IHC), using very 
   expensive primary antibodies and fairly expensive kits to amplify and 
   detect the binding sites. IHC is necessary if you must find every 
   macrophage, including a tissue's recent monocyte immigrants that haven't 
   yet done any work. Macrophages that have been busily eating blood are 
   full of brown granules that don't need any staining. 
   
   Ordinary people recognize macrophages by their appearance in sections 
   stained with a well done HE or with one of the many Romanowsky-Giemsa 
   blood stains. With the latter it's important to get the pH right - much 
   lower for sections of formaldehyde-fixed objects (pH4 to 5) than for 

Re: [Histonet] Fwd: foxp3 ab ihc

[Marilyn Tyler]

Thanks to all in Histonet world posted on Heathers behalf who is still having 
problems with her access
Marilyn

 Heather McCleod 2009/11/19 10:43 AM 
Sorry to bother you Marilyn.  Please forward. I have an answer, i.e. 
confirmation of what I would like to use.
 
Thanks to all who replied to my foxp3 query.
Heather

 Marilyn Tyler marilyn.ty...@uct.ac.za 2009/11/18 09:47 AM 

Does anybody in histoland use any FOXP3 antibody.  I would like to know which 
is the better ab to use for FFPE human material.
Posting on behalf of Heather as she cannot get through to Histonet
 Heather McCleod 2009/11/18 08:37 AM 


Thank you
The Histonet ROCKS!!!
Heather
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RE: [Histonet] Fwd: foxp3 ab ihc

[Tony Henwood]

Marilyn,

We have used Biolegend's Rabbit anti FoxP3 (Cat No 623801) at a 1/20
dilution with citrate retrieval (ER1) on the Bond Max with very good
results.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager  Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marilyn
Tyler
Sent: Wednesday, 18 November 2009 6:48 PM
To: histonet
Subject: [Histonet] Fwd: foxp3 ab ihc



Does anybody in histoland use any FOXP3 antibody.  I would like to know
which is the better ab to use for FFPE human material. Posting on behalf
of Heather as she cannot get through to Histonet
 Heather McCleod 2009/11/18 08:37 AM 
 
 
Thank you
The Histonet ROCKS!!!
Heather
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[Histonet] Fwd: foxp3 ab ihc

[Marilyn Tyler]

Does anybody in histoland use any FOXP3 antibody.  I would like to know which 
is the better ab to use for FFPE human material.
Posting on behalf of Heather as she cannot get through to Histonet
 Heather McCleod 2009/11/18 08:37 AM 
 
 
Thank you
The Histonet ROCKS!!!
Heather
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[Histonet] [Fwd: [PPMA] NSH meeting] Barcoding

[Victor Tobias]

For those of you interested in barcoding, this might be a worthwhile 
talk. Yes, he is my boss so I should put in a plug for him.


Victor

 Original Message 
Subject:[PPMA] NSH meeting
Date:   Wed, 30 Sep 2009 17:17:37 -0700 (Pacific Daylight Time)
From:   Rodney Schmidt schmi...@u.washington.edu
Reply-To: 	A mutual assistance forum for PowerPath users 
p...@u.washington.edu

To: PowerPath Mutual Assistance p...@u.washington.edu



For those of you attending the NSH meeting this next week, I hope to see 
some of you there.  I'll be giving a talk on Tuesday morning about the 
quality and efficiency benefits of barcoding in the AP lab.


If you'd like to get together for dinner or meet some other time, please 
drop me an off-line email.  Unfortunately, my stay in Birmingham is going 
to be fairly short -- from Monday afternoon until Tuesday afternoon.


See you there!


Rodney A. Schmidt, M.D., Ph.D.
Professor of Pathology
Director of Medical Informatics
University of Washington, Seattle, WA
(206) 598-6462
(206) 344-0532 (pager)
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[Histonet] Fwd: Who do you know for this great opportunity? - Long Island Histology Supe...

[DDittus787]

I am forwarding this for anyone looking for an opportunity. Please respond  
directly to the recruiter at the bottom of the job description.
 
dana
**A Good Credit Score is 700 or Above. See yours in just 2 easy 
steps! 
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[Histonet] Fwd: Can you place on the histonet?

[LINDA MARGRAF]

Here's a message Carol wanted posted to the list.  Please respond to her and 
not me. Thanks
Linda M
Histonet administrator

 Barone, Carol  cbar...@nemours.org 2/13/2009 10:33 AM 

Anyone using bead detection systems with readers for tissue histology. I have 
investigators interested in these for slides? Need infoFAST...for grant 
submission...Help
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[Histonet] Fwd: Texas Society for Histotechnology 2009 Call for Abstracts

[kdwyer3322]

-Original Message-
From: [EMAIL PROTECTED]
To: histonet@lists.utsouthwestern.edu
Sent: Sun, 2 Nov 2008 7:04 pm
Subject: Texas Society for Histotechnology 2009 Call for Abstracts



Histonetters, 

The Texas Society for Histotechnology will be holding it 2009 meeting in 
beautiful Austin Texas on May 28-31, 2009 (This is not Memorial weekend) at the 
Hyatt Regency Austin Lady Bird Lake, 208 Barton Springs, Austin, Texas.? 

The program committee is actively seeking workshop/symposum abstracts for this 
meeting.? If you or anyone you know would like to submit a workshop to the 
meeting please contact: 

Kathy Dwyer?- [EMAIL PROTECTED] ?
OR
Veronica Davis - [EMAIL PROTECTED]

Attached is the TSH 2009 Call for Abstracts. 

Thanks, 
TSH Convention Committe


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