Dear Dirk
For REFMAC, I
haven't seen anything similar, yet.
The external restraints in Refmac should allow you to do this.
http://www.ysbl.york.ac.uk/~garib/refmac/data/refmac_news.html
Best
Roberto
Hi All,
I would greatly appreciate your comments on following:
I would like to know the quantitative relation between Solvent
excluded area (calculated by Areaimol at CCP4i) and free energy of
binding or binding affinity of the DNA-protein or protein-protein
interactions.
Is there some free
Hi,
are you sure it is your protein precipitating? You will get a cloudy
precipitate appearing in HEPES and Phosphate buffers on addition of ZnCl,
without protein.
cheers
charlie
dengzq1987 dengzq1...@gmail.com 3/31/2010 5:08 pm
hello everyone, recently i purify a protein conteining
PISA can give you such estimate, you may try standalone pisa in CCP4 or
on-line (google on EBI PISA)
Eugene
On Thu, Apr 1, 2010 at 8:36 AM, Mike England england@gmail.com wrote:
Hi All,
I would greatly appreciate your comments on following:
I would like to know the quantitative
What is 1mM BTW? I am not familiar with this abbreviation.
PBS phoshate buffered saline (Phosphate + NaCl) not suitable for zinc
binding proteins
BBS borate buffered saline (Borate + NaCl) wrong pH for zinc binding.
BTW ? (? ? ?) No idea what this is. Charles, do you know if it includes
BTWBy the way I think, not a buffer abbreviation
--
AstraZeneca UK Limited is a company incorporated in England and Wales with
registered number: 03674842 and a registered office at 15 Stanhope Gate, London
W1K 1LN.
I have no idea, it is not my buffer, it is dengzq1987's!
'BTW' means 'by the way', BTW.
cheers,
charlie
Enrico Stura est...@cea.fr 4/1/2010 11:01 am
What is 1mM BTW? I am not familiar with this abbreviation.
PBS phoshate buffered saline (Phosphate + NaCl) not suitable for zinc
binding
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