Re: [ccp4bb] Two Postdoc positions at the University of Oxford, SGC

Gentle reminder of the following openings at SGC Oxford (deadline 7th February noon). Thanks. On 14 January 2013 17:51, Wyatt W. Yue yuewai...@googlemail.com wrote: Dear all, We are seeking two post-doc scientists in the Metabolic Rare Diseases group at the Structural Genomics Consortium

Re: [ccp4bb] generating electron density from PDB and structure factor file

Just to add some more possibilities: - You can download maps from EDS or models and maps from PDB_REDO straight into CCP4mg. - You can download PDB_REDO maps and models into PyMOL using this plugin (http://www.cmbi.ru.nl/pdb_redo/pymol.html) for which we should thank Ed Pozharski. Note that

[ccp4bb] Pilatus 300K CBF to Oxford Diffraction format convert

Dear all, sorry for the semi-off-topic but I'm trying to help convert some diffraction images and this seemed like a good place to ask. We are trying to process some images collected on a Pilatus 300K with the Crysalis Pro software (small molecule) but it seems to only be able to read Pilatus

Re: [ccp4bb] Pilatus 300K CBF to Oxford Diffraction format convert

-BEGIN PGP SIGNED MESSAGE- Hash: SHA1 Dear Jose, it is odd the software should read 6M but not 0.3M Pilatus files - the format is probably the same, only the dimensions would differ - at least that's my guess. While you are waiting you might start processing your data with XDS, which is

Re: [ccp4bb] Pilatus 300K CBF to Oxford Diffraction format convert

Hi Tim I've looked at these images and they differ from the normal 6M images (miniCBF) in that they are a stab at writing fullCBF, i.e. with imgCIF style data content - unfortunately, there are a few syntax errors which need fixing before programs that use the header information (like

Re: [ccp4bb] COOT v. 0.7

-BEGIN PGP SIGNED MESSAGE- Hash: SHA1 Hello Xavier, if you look at the downloaded file, you'll notice the source of the error: The pdbe download link has changed, i.e. this is not a PDB-file. You'll have to download the PDB file manually until it is fixed in the latest coot-code.

[ccp4bb] Off Topic- Cystine Detection

Dear All, I am trying to probe the existence of a disulfide bond on the surface of my protein. I have attempted Ellman´s and my results were not as clear as I would have hoped for. I am not a sulfur/cysteine chemist and would appreciate the advice on what experiments to try! Thanks a bunch YAP

Re: [ccp4bb] Off Topic- Cystine Detection

I never done such experiments but N-Ethylmaleimide labeling and Mass-Spec could be the solution. Le 6 févr. 2013 à 17:10, Yuri Pompeu yuri.pom...@ufl.edu a écrit : Dear All, I am trying to probe the existence of a disulfide bond on the surface of my protein. I have attempted Ellman´s and

[ccp4bb] Beamtime @ SLS

=== SYNCHROTRON BEAM TIME FOR MACROMOLECULAR CRYSTALLOGRAPHY AT SLS === Proposal application deadline: Friday, February 15, 2013 Periods: May 1, 2013 - August

Re: [ccp4bb] Off Topic- Cystine Detection

Hi, to my knowledge, Ellman's reagent detects free thiols only. (Or am I wrong?) In contrast, with the method of Thannhauser (Thannhauser [1987], Methods Enzymol. 143, 115-9) you can determine the total amount of thiols (free and in disulfide bonds). Any difference between the two methods

Re: [ccp4bb] Off Topic- Cystine Detection

Couldn't you just run reducing/non-reducing SDS-PAGE lanes and see the difference? JPK On Wed, Feb 6, 2013 at 11:10 AM, Yuri Pompeu yuri.pom...@ufl.edu wrote: Dear All, I am trying to probe the existence of a disulfide bond on the surface of my protein. I have attempted Ellman´s and my

Re: [ccp4bb] need some suggestions for crystallization

Does that CA have a metal center (I think they all do)? If so, doesn't the citrate compete for the metal? JPK On Mon, Feb 4, 2013 at 2:25 PM, Roger Rowlett rrowl...@colgate.edu wrote: Human carbonic anhdyrase II can be easily crystallized from 1.3 M sodium citrate/0.1 M TrisCl pH 8.5 at 10

Re: [ccp4bb] Off Topic- Cystine Detection

*** This message has been scanned by the InterScan for CSC SSM by IICT security policy and found to be free of known security risks. *** Dear Yuri, If you have access to mass spec, this should be a straight forward experiment. Find the reference here.