Re: [ccp4bb] Fwd: [ccp4bb] Fwd: [ccp4bb] expanding reflections from C2221 to P21

QUICK ERRATUM Sorry W, I forgot a very important bit (I was half asleep ;-)). As the unit cells are, it would not be correct to import one set of Rfree flags from one dataset to the other in sftools as the axes are moved around and the indexing is no longer consistent between the two mtzs.

Re: [ccp4bb] Fwd: [ccp4bb] Fwd: [ccp4bb] expanding reflections from C2221 to P21

CAVEAT The procedure discussed in previous email to try to simply import one set of Rfree flags from on mtz to the other, however, would required a very carefully choice of matching unit cells origins ... This problem would be overcome by expanding the orthorombic lattice to the primitive

[ccp4bb] X-ray pattern has streaks

I introduce myself as Dr.Krithika Gokulnath, CAS in crystallography and Biophysics, UNiversity of Madras. I have been working with a cloned protein and have been successfully able to crystallise it. However data collection done at home source shows a pattern of streaks instead of spots. The

Re: [ccp4bb] X-ray pattern has streaks

Dear Krithika, what you probably see is the diffraction pattern of a highly mosaic crystal due to probably internal disordering. cheers Demetres On 3/10/2013 3:40 μμ, Krithika GOkulnath wrote: I introduce myself as Dr.Krithika Gokulnath, CAS in crystallography and Biophysics, UNiversity

Re: [ccp4bb] Identity of a Bacterial lipid

Lipid or PEG from your crystallisation condition? --- Dr Antony W Oliver Senior Research Fellow CR-UK DNA Repair Enzymes Group Genome Damage and Stability Centre Science Park Road University of Sussex Falmer, Brighton, BN1 9RQ email: antony.oli...@sussex.ac.uk tel (office): +44 (0)1273 678349

Re: [ccp4bb] Identity of a Bacterial lipid

Dear Andre, It always impressive to see a near atomic resolution structure with a bound lipid. Congratulations. However, to identify what lipid you have requires a bit more analysis than just looking in databases. First, what is the bacterium you are using as the host? If it is E. coli,

[ccp4bb] RES: [ccp4bb] Identity of a Bacterial lipid

Dear Michael, thank you for the prompt reply. The host was indeed E. coli. From what I have been reading I completely agree on the lack of biological support for such a molecule but still the map seems very convincing of the presence of cis bonds at such positions... Could such a conformation

Re: [ccp4bb] RES: [ccp4bb] Identity of a Bacterial lipid

When USA budget is back :-\ you may give a try to : http://webbook.nist.gov/chemistry/ It helped me several times, very intuitive, I use just a formula for a search, it also accept a wild character for unknown number of atoms. FF Dr Felix Frolow Professor of Structural Biology and

Re: [ccp4bb] Adding ligand to crystallization drop

Hi Chris, Though there is no alternative to have a co-crystal structure it does not always happened to be the ideal case where you would easily get it co-crystallized in the same condition. Soaking worked well in many cases, though definitely the answer is very dependent on a particular case to

Re: [ccp4bb] X-ray pattern has streaks

One standard thing to do would be to set up a grid around the condition and then try picking crystals from different well to see if any of them produce better looking diffraction patterns. If that does not work one might give any of the commercially available additive screen a try to see if

[ccp4bb] yeast type I transmembrane protein

Hi all, Sorry for the non-CCP4 question. I am looking for a type I transmembrane protein in yeast, which resides on the plasma membrane instead of ER or Golgi. A quick search in the database returns a large number of transmembrane proteins with multiple TMs or targeted to various compartments.

[ccp4bb] Beam time at CHESS

== Beamtime available at CHESS, October 16 - December 13, 2013 == The CHESS/MacCHESS facility, located at Cornell University in Ithaca, NY, invites macromolecular

Re: [ccp4bb] X-ray pattern has streaks

Yes, and the mosaicity might have been poor to start with, or it might have been caused by sub-optimal freezing conditions, assuming that the crystal was frozen (not enough information to tell). I recommend doing a room temperature diffraction experiment to see which it is and act accordingly -

Re: [ccp4bb] X-ray pattern has streaks

Depends a bit on what the streaks look like. If they are curved, and your streaks are connecting partial rings at different resolutions, then what you may be seeing is simply diffuse scatter from a small molecule, such as ice. The only way to be sure you are looking at protein is if you see

Re: [ccp4bb] OT:Website help

Hi Bernhard, I manage our websites (including 'c6.csiro.au') and have some experience of your situation, as earlier this year we upgraded our server from IIS 6.0 - IIS 8.0 I don't know the full details of what your setup is, (e.g. I am assuming you have full access to the machine, it is not