[ccp4bb] Postdoctoral positions at Karolinska Institutet, Stockholm

2024-01-05 Thread Luca Jovine
, please visit: https://ki.varbi.com/en/what:job/jobID:687262 You are of course always welcome to also contact me directly for informal queries. With best wishes for a successful and inspiring start to the new year, we look forward to receiving your application! Luca -- Luca Jovine, Ph.D

Re: [ccp4bb] mmciif pdb file editor.

2023-08-31 Thread Luca Jovine
https://pdbj.org/cif-editor/ HTH, Luca From: CCP4 bulletin board on behalf of Krishnan Raman Reply to: Krishnan Raman Date: Wednesday, 30 August 2023 at 15:06 To: "CCP4BB@JISCMAIL.AC.UK" Subject: [ccp4bb] mmciif pdb file editor. You don't often get email from rkrish...@biocryst.com. Learn

Re: [ccp4bb] Mac M2 chip

2023-07-05 Thread Luca Jovine
Hi Firdous, for Phaser the solution is simple - just install any nightly version of PHENIX > 1.21rc1-5015 (from https://phenix-online.org/download/nightly_builds.cgi?show_all=1), and it should be fine. -Luca From: Firdous Tarique Date: Wednesday, 5 July 2023 at 18:33 To: Luca Jovine

Re: [ccp4bb] Mac M2 chip

2023-07-05 Thread Luca Jovine
Hi Dan, Please see yesterday's posts on phenixbb (thread https://phenix-online.org/pipermail/phenixbb/2023-July/025527.html): there was an issue with Phaser, but this has now been fixed in and it runs superfast. There may be others, but whatever I've been throwing at my M2 in recent times

[ccp4bb] Two postdoctoral openings in structural biochemistry of fertilization at Karolinska Institutet

2023-06-28 Thread Luca Jovine
for informal queries. We look forward to receiving your application! With best regards, Luca -- Luca Jovine, Ph.D. Professor of Structural Biology, Member of EMBO and the Nobel Assembly Karolinska Institutet Department of Biosciences and Nutrition Medicinaren 25 Neo Blickagången 16, SE-141 83

Re: [ccp4bb] Structure prediction - waiting to happen

2023-04-02 Thread Luca Jovine
This was published just recently and it's really informative: https://pubmed.ncbi.nlm.nih.gov/36876433/ Best, Luca From: CCP4 bulletin board on behalf of Ian Tickle Sent: Sunday, April 2, 2023 3:30 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Structure

[ccp4bb] Postdoctoral position in structural biology of fertilization at Karolinska Institutet, Stockholm

2021-07-27 Thread Luca Jovine
application, Luca -- Luca Jovine, Ph.D. Professor of Structural Biology & EMBO Member Karolinska Institutet Department of Biosciences and Nutrition SE-141 83 Huddinge, Sweden E-mail: luca.jov...@ki.se W3: http://jovinelab

Re: [ccp4bb] Coming July 29: Improved Carbohydrate Data at the PDB -- N-glycans are now separate chains if more than one residue

2020-12-04 Thread Luca Jovine
CIF capability for > multi-character chain IDs, and name them by appending characters to the > parent chain ID? Using chain A as an example, perhaps the glycans could > become Ag1, Ag2, etc.? > >> On 4 Dec 2020, at 07:48, Luca Jovine >> mailto:luca.jov...@ki.se>> wro

Re: [ccp4bb] Coming July 29: Improved Carbohydrate Data at the PDB -- N-glycans are now separate chains if more than one residue

2020-12-04 Thread Luca Jovine
n. A > suggestion: why not take full advantage of the mmCIF capability for > multi-character chain IDs, and name them by appending characters to the > parent chain ID? Using chain A as an example, perhaps the glycans could > become Ag1, Ag2, etc.? > >> On 4 Dec 2020, at 07:4

Re: [ccp4bb] Coming July 29: Improved Carbohydrate Data at the PDB -- N-glycans are now separate chains if more than one residue

2020-12-03 Thread Luca Jovine
CC: pdb-l Dear Zhijie and Robbie, I agree with both of you that the new carbohydrate chain assignment convention that has been recently adopted by PDB introduces confusion, not just for PDB-REDO but also - and especially - for end users. Could we kindly ask PDB to improve consistency by

[ccp4bb] Postdoctoral positions in structural biology of fertilization at Karolinska Institutet and Umeå University

2020-07-06 Thread Luca Jovine
ajor implications for the future of human reproductive medicine! With best wishes, Luca -- Luca Jovine, Ph.D. Professor of Structural Biology, Member of EMBO and the Nobel Assembly Karolinska Institutet Department of Biosciences and

[ccp4bb] Postdoctoral studies in structural biology of fertilization, Karolinska Institutet

2020-02-07 Thread Luca Jovine
Luca ---- Luca Jovine, Ph.D. Professor of Structural Biology & EMBO Member Karolinska Institutet Department of Biosciences and Nutrition Medicinaren 25 Neo Blickagången 16, SE-141 83 Huddinge, Sweden E-mail: luca.jo

Re: [ccp4bb] Live stream of CCP4 Study Weekend?

2019-01-09 Thread Luca Jovine
re appearing at bit.ly/2H5Nr51<http://bit.ly/2H5Nr51> pic.twitter.com/6tLLc53Cnh<http://pic.twitter.com/6tLLc53Cnh> -Luca ---- Luca Jovine, Ph.D. Professor of Structural Biology & EMBO Member Karolinska Institut

Re: [ccp4bb] Non-specific disulfides in a secreted protein

2018-12-15 Thread Luca Jovine
/pubmed/24680733 We have also observed this phenomenon several times, and simply diluting the expression construct did indeed help in such cases. Good luck! Luca Luca Jovine, Ph.D. Professor of Structural Biology &

[ccp4bb] REMINDER - Postdoc position in structural biology of TGF-beta superfamily coreceptors, Karolinska Institutet (deadine 3 September 2017)

2017-08-18 Thread Luca Jovine
Dear all, The research group of Luca Jovine at Karolinska Institutet has an opening for a postdoctoral fellow to study protein complexes involving TGF-beta superfamily coreceptors, with a focus on endoglin/CD105 - a key human glycoprotein involved in hereditary hemorrhagic telangiectasia type

[ccp4bb] Postdoc position in structural biology of TGF-beta superfamily coreceptors, Karolinska Institutet

2017-07-12 Thread Luca Jovine
Dear all, The research group of Luca Jovine at Karolinska Institutet has an opening for a postdoctoral fellow to study protein complexes involving TGF-beta superfamily coreceptors, with a focus on endoglin/CD105 - a key human glycoprotein involved in hereditary hemorrhagic telangiectasia type

Re: [ccp4bb] Crystallisation of a minority fraction monomers

2015-04-08 Thread Luca Jovine
Sure - crystallization selects what packs, which may constitute a very minor fraction of what you set up drops with. 1DUH is an example... -Luca On Apr 8, 2015, at 15:08, Sebastiaan Werten sebastiaan.wer...@uni-greifswald.de wrote: Dear all, we are currently working on a protein that is

[ccp4bb] Postdoctoral position at Karolinska Institutet

2014-06-13 Thread Luca Jovine
A postdoctoral position is available immediately on structural studies of protein-protein interactions at the core of the human Hedgehog signaling pathway, as part of a long-term collaboration between the laboratories of Rune Toftgård and Luca Jovine at the Department of Biosciences

Re: [ccp4bb] Molecular cloning software for linux

2013-02-08 Thread Luca Jovine
: http://serialbasics.free.fr/Serial_Cloner.html We use the OS X version and it's great (and free)! Best, Luca Luca Jovine, Ph.D. Assistant Professor EMBO Young Investigator Karolinska Institutet Department of Biosciences

[ccp4bb] Postdoctoral position at Karolinska Institutet

2012-11-28 Thread Luca Jovine
As part of a long-term collaboration between the laboratories of Luca Jovine and Rune Toftgård at the Department of Biosciences and Nutrition the Center for Biosciences of Karolinska Institutet, a postdoctoral position is available from January 2013 on structural studies of protein-protein

Re: [ccp4bb] Various OSes and Crystallography

2012-08-09 Thread Luca Jovine
Yes, you can define 3 buttons on a MagicMouse with this free software: http://magicprefs.com/ or simply attach a mouse with 3 real buttons... -Luca Luca Jovine, Ph.D. Assistant Professor EMBO Young Investigator

Re: [ccp4bb] Off-topic His-Antibody

2012-06-26 Thread Luca Jovine
On 26 June 2012 06:59, Luca Jovine luca.jov...@ki.se wrote: We routinely use BSA-free anti-5His mAb from QIAGEN, and it works just fine (at least using media and lysates of both insect and mammalian cells): http://www.qiagen.com/products/protein/detection/qiaexpressdetectionsystem

Re: [ccp4bb] Off-topic His-Antibody

2012-06-25 Thread Luca Jovine
Luca Jovine, Ph.D. Assistant Professor EMBO Young Investigator Karolinska Institutet Department of Biosciences and Nutrition Center for Biosciences Hälsovägen 7, SE-141 83 Huddinge, Sweden Voice: +46.(0)8.524-81136 FAX: +46.(0)8.6081-501 E-mail: luca.jov

Re: [ccp4bb] Off topic: His-tag purification

2012-01-16 Thread Luca Jovine
…alternatively, depending on what is the source of your protein, you might have to dialyze the (concentrated) sample before applying it to IMAC. Insect cell media, in particular, can be pretty good at stripping off Ni2+ from IMAC supports. HTH, Luca

Re: [ccp4bb] is codon optimization worth it?

2011-09-30 Thread Luca Jovine
Luca Jovine, Ph.D. Assistant Professor EMBO Young Investigator Karolinska Institutet Department of Biosciences and Nutrition Center for Biosciences Hälsovägen 7, SE-141 57 Huddinge, Sweden Voice: +46.(0)8.524-81136 FAX: +46.(0)8.6081-501 E-mail: luca.jov...@ki.se W3: http

Re: [ccp4bb] Off topic: vector map editing and DNA sequence alignment software

2011-09-27 Thread Luca Jovine
://serialbasics.free.fr/Serial_Cloner.html HTH, Luca Luca Jovine, Ph.D. Assistant Professor EMBO Young Investigator Karolinska Institutet Department of Biosciences and Nutrition Center for Biosciences Hälsovägen 7, SE-141 83 Huddinge

Re: [ccp4bb] Setup pymol in Mac OS X 10.7

2011-09-21 Thread Luca Jovine
Luca Jovine, Ph.D. Assistant Professor EMBO Young Investigator Karolinska Institutet Department of Biosciences and Nutrition Center for Biosciences Hälsovägen 7, SE-141 57 Huddinge, Sweden Voice: +46.(0)8.524-81136 FAX: +46.(0)8.6081-501 E-mail: luca.jov

Re: [ccp4bb] metal binds?

2011-03-25 Thread Luca Jovine
Screening for phasing atoms in protein crystallography Structure. 2000 Jul 15;8(7):R143-9 http://www.ncbi.nlm.nih.gov/pubmed/10903954 HTH, Luca Luca Jovine, Ph.D. Assistant Professor EMBO Young Investigator Karolinska

[ccp4bb] Postdoctoral position in structural biology of fertilization proteins at Karolinska Institutet

2010-04-30 Thread Luca Jovine
offer a highly international and collaborative environment for biomedical studies. Highly motivated candidates are encouraged to apply by e-mailing a curriculum vitae, summary of research experience and interests, and names and contact information for two references to Luca Jovine (luca.jov

[ccp4bb] Postdoctoral position in protein crystallography at Karolinska Institutet

2009-07-30 Thread Luca Jovine
POSTDOCTORAL FELLOWSHIP IN PROTEIN CRYSTALLOGRAPHY AT KAROLINSKA INSTITUTET As part of a collaboration between the laboratories of Rune Toftgård and Luca Jovine at the KI Department of Biosciences and Nutrition and the Center for Biosciences, a postdoctoral position is immediately available

Re: [ccp4bb] generating omit maps

2008-12-10 Thread Luca Jovine
was wondering if there's a CCP4 related program to generate omit maps. Thanks, Kathleen Hi Kathleen, Not CCP4, I know, but this can be done very easily in PHENIX: http://www.phenix-online.org/documentation/autobuild.htm#anch159 HTH, Luca Luca Jovine

[ccp4bb] Postdoctoral position in protein crystallography at Karolinska Institutet

2008-01-14 Thread Luca Jovine
POSTDOCTORAL POSITION IN PROTEIN CRYSTALLOGRAPHY AT KAROLINSKA INSTITUTET As part of a collaboration between the laboratories of Rune Toftgård and Luca Jovine at the KI Department of Biosciences and Nutrition at Novum, a postdoctoral position is immediately available to study

[ccp4bb] Postdoctoral position in protein crystallography at Karolinska Institutet (correction)

2008-01-14 Thread Luca Jovine
CRYSTALLOGRAPHY AT KAROLINSKA INSTITUTET As part of a collaboration between the laboratories of Rune Toftgård and Luca Jovine at the KI Department of Biosciences and Nutrition at Novum, a postdoctoral position is immediately available to study the structure of a key player within the Hedgehog signaling

[ccp4bb] C3b, validation, deposition diffraction images

2007-08-17 Thread Luca Jovine
. Luca Jovine, Ph.D. Karolinska Institutet Department of Biosciences and Nutrition Hälsovägen 7, S-141 57 Huddinge, Sweden Voice: +46.(0)8.6083-301 FAX: +46.(0)8.6089-290 E-mail: [EMAIL PROTECTED] W3: http://www.ki.se/

Re: [ccp4bb] MarView on OSX

2007-01-26 Thread Luca Jovine
./marView HTH, Luca - Luca Jovine, Ph.D. Karolinska Institutet Department of Biosciences and Nutrition Center for Structural Biochemistry (CSB) Hälsovägen 7, S-141 57 Huddinge, Sweden Voice: +46.(0)8.6083-301 FAX: +46.(0)8.6089-290 E-mail: [EMAIL