Re: [ccp4bb] Best (Suitable) Mac Laptop configuration for protein Xtallography

Dear Mac Lovers: I would have to agree with Jurgen with SSD drives. These fast drives have breathed new life into my old Macs (2011 Macbook Pro, 2009 Mac mini, and yes a 2006 Mac Pro (has 8 cores and just keeps running)). In addition, all these Macs are maxed out with RAM. Cheers, Scott

Re: [ccp4bb] Bin R and Rfree values

Hi Faisil, Looking at your table, I think your data can go to even higher resolution than you report. Did you setup your data collection for higher resolution? Cheers, Scott Scott T. R. Walsh, Ph.D. Assistant Professor University of Maryland

Re: [ccp4bb] Molecular Replacement model preparation

Hi Steven, Thank you for the information and guidance. When you search for all the ensembles with Phaser do you use “AND” or “OR” searching? Cheers, Scott While Phil Jeffrey attributed to me the “trick” of aligning the hinge axis of an Fab along the Z direction, I, in turn, must give

Re: [ccp4bb] Molecular Replacement model preparation

Hi Phil, Thank you for the input. I would like to get CCP4ers input. I deal with multiple domain cytokine receptors in a manner very similar to antibody molecules. Have people have more correct solutions searching for 1 domain sequentially and then other domains OR searching for multiple

Re: [ccp4bb] Phasing with Many Monomers/AU

Hi Chris, It would be nice to have a wee bit more information. Is 3.4-3.0 angstroms from a home source or synchrotron? What are the crystallization conditions for both the native and SeMet crystals? Did you see the SeMet crystals with the native crystals. Have you tried MMS with the native

Re: [ccp4bb] stereo set up

Hi Hena, The 3D stereo on PyMOL and Coot are being driven through OpenGL, not Direct X support on your current laptop setup. Scott Scott T. R. Walsh, PhD Assistant Professor University of Maryland IBBR/CBMG 3127E CARB-2 9600 Gudelsky Drive

Re: [ccp4bb] NCS from electron density

Hi Francis, Do you have a specific how to on your experience with NCS (reference)? Cheers, Scott Scott T. R. Walsh, PhD Assistant Professor University of Maryland IBBR/CBMG 3127E CARB-2 9600 Gudelsky Drive Rockville, MD 20850 USA phone: (240)

Re: [ccp4bb] Izit dye stained crystal

Hi Sarathy, What does the density modified electron density map after phasing from either autoSHARP or Phenix? Can the auto building programs build protein chains into this map? Cheers, Scott On Nov 30, 2012, at 5:27 PM, Sarathy Karunan Partha

Re: [ccp4bb] Question on stereo monitor: LG D2342P-PN

Hi Zhijie, Is the LG D2342P-PN screen glossy or anti-glare surface? The Zalman ZM-M240W I have is glossy. Thank you. Scott Scott T. R. Walsh, Ph.D. Assistant Professor University of Maryland IBBR/CBMG Rm 3127E CARB-2 9600 Gudelsky Drive

Re: [ccp4bb] advice on spectrometric instruments choice

Hi Sebastiano, We have a Chirascan instrument with all the attachments (fluorescence, stopped flow, multi-cell attachment, etc). It is by far the best CD instrument that I have used. I have experience in the past with Aviv, Jasco, and Olis instruments. Cheers, Scott

Re: [ccp4bb] Off Topic: Isothermal Titration Calorimetry (ITC)

Dear Jose, I have an iTC200 in my lab and have experience with a VP-ITC. The major problem with the iTC200 is that GE has not increased the sensitivity of the instrument. Thus, you must use higher concentrations in both the cell and syringe to see measurable heats. The syringe on the iTC200

Re: [ccp4bb] OSX and stereo

Works fine with MacPyMOL v. 1.5. You have to copy MacPyMOL.app to MacPyMOLZalman.app and fire this off. Cheers, Scott Scott T. R. Walsh, Ph.D. Assistant Professor University of Maryland College Park Institute for Bioscience and Biotechnology