Re: [ccp4bb] A basic question about Fourier Transform

2015-01-21 Thread Zhijie Li
, 2015 10:18 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] A basic question about Fourier Transform Dear all, I am sorry about this slightly off-topic question. I am now a graduate TA for crystallography course and one student asked me a question that I didn't ask myself before. I don't have

Re: [ccp4bb] A basic question about Fourier Transform

2015-01-21 Thread Keller, Jacob
] On Behalf Of Chen Zhao Sent: Tuesday, January 20, 2015 11:47 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] A basic question about Fourier Transform Dear Steven, Thank you for your reply! I understand that it is nearly impossible to measure the diffraction of a single molecule, and I am just

Re: [ccp4bb] A basic question about Fourier Transform

2015-01-21 Thread Chen Zhao
bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of *Chen Zhao *Sent:* Tuesday, January 20, 2015 11:47 PM *To:* CCP4BB@JISCMAIL.AC.UK *Subject:* Re: [ccp4bb] A basic question about Fourier Transform Dear Steven, Thank you for your reply! I understand that it is nearly impossible

Re: [ccp4bb] A basic question about Fourier Transform

2015-01-20 Thread Steven Chou
I would say you cannot measure the diffraction pattern of a single biological molecule accurately thus far, because biological molecules are not strong scatters and can be damaged easily. For other molecules, actually you can! In high-resolution electron microscopy, the diffraction pattern in the

Re: [ccp4bb] A basic question about Fourier Transform

2015-01-20 Thread Keller, Jacob
The question is, as I rephrased it, assuming we are able to measure the diffraction pattern of a single molecule with acceptable accuracy and precision (comparable to what we have now for the common crystals), is it better than we measure the diffraction spots from a crystal, given that the

Re: [ccp4bb] A basic question about Fourier Transform

2015-01-20 Thread Chen Zhao
Dear Steven, Thank you for your reply! I understand that it is nearly impossible to measure the diffraction of a single molecule, and I am just bringing this up as a thought experiment to help understand the basics in crystallography. But I never thought that some molecules actually allow such

Re: [ccp4bb] A basic question about Fourier Transform

2015-01-20 Thread Chen Zhao
Hi Jacob, Thanks a lot for your reply! Yes, by comparable data quality I did mean the comparable resolution and SNR. I now understand the original question and kinda confirm what I thought. But I am also learning myself and I don't quite get why the continuous sampling would get rid of the phase

Re: [ccp4bb] A basic question about Fourier Transform

2015-01-20 Thread Ethan Merritt
On Tuesday, 20 January 2015 10:18:35 PM Chen Zhao wrote: Dear all, I am sorry about this slightly off-topic question. I am now a graduate TA for crystallography course and one student asked me a question that I didn't ask myself before. I don't have enough knowledge to precisely answer this